Review
Biochemistry & Molecular Biology
FengXia Gao, SiRong He, AiShun Jin
Summary: lncRNAs and miRNAs play significant roles in NK cell biology and diseases, serving as potential biomarkers/targets for early diagnosis, targeted treatment, and prognostic evaluations of NKTL.
Article
Reproductive Biology
Bereket Molla Tanga, Xun Fang, Seonggyu Bang, Gyeonghwan Seong, Mahanama De Zoysa, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho
Summary: This study investigated the effect of miRNA-155 on parthenogenically activated early development porcine embryos in vitro. miRNA-155 inhibition improved in vitro developmental competence by increasing cell numbers and reducing stress and apoptosis levels. The cleavage rate and blastocyst rate in the miRNA-155 inhibitor group were significantly higher than in the control and miRNA-155 mimic groups.
Review
Immunology
Massimo Fantini, Philip Martin Arlen, Kwong Yok Tsang
Summary: Natural killer (NK) cells are important components of the innate immune system that can recognize and suppress the proliferation of cancer cells. Strategies such as boosting NK cells with modulatory cytokines, adoptive NK cell therapy, and the combination of engineered NK cells with monoclonal antibodies (mAbs) that mediate antibody-dependent cell-mediated cytotoxicity (ADCC) have been developed to enhance the antitumor activity of NK cells. The combination of engineered NK cells with mAbs with higher affinity for CD16 expressed on NK cells shows promise in providing more effective and higher-quality treatments for cancer patients.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Supriya Prakash, Ninad Kumbhojkar, Andrew Lu, Neha Kapate, Vineeth Chandran Suja, Kyung Soo Park, Lily Li-Wen Wang, Samir Mitragotri
Summary: Natural killer (NK) cell therapies have limitations in efficacy due to low persistence and anergy. This study introduces a material-based approach called microparticles as cell engagers (MACE), which uses polymeric micropatches to sustain and activate NK cells. The activated NK cells bound to MACE exhibit viability, transendothelial migration, and antitumor activity. MACE also activates other immune cells and shows superior antitumor efficacy in a mouse melanoma lung metastasis model. Overall, MACE offers a simple and effective platform to improve NK cell therapy.
Article
Immunology
Bert Malengier-Devlies, Jessica Filtjens, Kourosh Ahmadzadeh, Bram Boeckx, Jessica Vandenhaute, Amber De Visscher, Eline Bernaerts, Tania Mitera, Cato Jacobs, Lore Vanderbeke, Pierre Van Mol, Yannick Van Herck, Greet Hermans, Philippe Meersseman, Alexander Wilmer, Mieke D. Gouwy, Abhishek Garg, Stephanie Humblet-Baron, Frederik De Smet, Kimberly Martinod, Els Wauters, Paul Proost, Carine Wouters, Georges Leclercq, Diether Lambrechts, Joost Wauters, Patrick Matthys
Summary: NK cells in COVID-19 patients display an activated phenotype and show significant differences between different stages of infection. Early-stage patients have increased expression of cytotoxic molecules, while late-stage patients show enhanced levels of cytokines. The activation of NK cells is correlated to neutrophil count and IL-15 levels, but cytotoxic killing is normal.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Kwang-Soo Kim, Dong-Hwan Kim, Dong-Hyun Kim
Summary: NK cell cancer immunotherapy plays a unique role but faces challenges such as the immune-suppressive tumor microenvironment, low activity of NK cells, and limited contact frequency of NK cells with tumor cells. Innovative strategies are needed to advance NK cell cancer immunotherapy.
Article
Infectious Diseases
Chen Liang, Shanshan Li, Jinfeng Yuan, Yanhua Song, Weicong Ren, Wei Wang, Yuanyuan Shang, Shenjie Tang, Yu Pang
Summary: This study aimed to investigate the phenotype, proliferation and functional alterations of cytokine-induced memory-like natural killer (CIML NK) cells from healthy subjects and TB patients, and assessed the efficacy of CIML NK cells in response to H37Rv-infected U937 cells in vitro. CIML NK cells from healthy individuals showed enhanced IFN-gamma secretion and boosted anti-MTB activity in vitro, which from TB patients show impaired IFN-gamma production and no enhanced anti-MTB activity compared to those from healthy donors. These results open up new possibilities for NK cell-based anti-tuberculosis immunotherapeutic strategies.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Biochemistry & Molecular Biology
Chantal Reina-Ortiz, M. Pilar Mozas, David Ovelleiro, Fei Gao, Martin Villalba, Alberto Anel
Summary: Therapies based on allogenic Natural Killer (NK) cells, specifically expanded and activated NK (eNK) cells, have shown significant cytotoxicity against hematological cancers. This study aims to understand the changes that occur in healthy NK cells during the expansion process in order to develop a more potent eNK-based therapy. MicroRNA (miRNA) expression analysis revealed changes in the expression of 64 miRNAs, highlighting the up-regulation of miRs-146a, -124, -34a, and -10a, which play a key role in cell survival, and the down-regulation of miRs-199a, -223, and -340, associated with increased NK cell cytotoxicity. Validation experiments confirmed the functional importance of these miRNAs and demonstrated the wide variety of changes in eNK cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Victor Camberos, Jonathan Baio, Ana Mandujano, Aida F. Martinez, Leonard Bailey, Nahidh Hasaniya, Mary Kearns-Jonker
Summary: The study investigated the transcriptome of cardiovascular progenitors cultured in space for 30 days, revealing that they acquire a gene expression profile representative of an early-stage, dedifferentiated, stem-like state regardless of age. Spaceflight induced signaling pathways that support cell proliferation and survival, along with transcripts related to cell cycle re-entry, cardiovascular development, and oxidative stress. These findings provide new insight into the multifaceted influence of reduced gravitational environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Grace Lee, Sheela Karunanithi, Bruce Posner, Hanspeter Niederstrasser, Hong Cheng, Yuriy Federov, Shivaprasad Manjappa, Karam Musaitif, Huaiyu Wang, Zachary Jackson, David Wald
Summary: Our work led to the identification of a promising compound that potently increases NK cell-mediated killing of a variety of different cancer cells, but has no impact on the killing of normal cells. This compound demonstrates the utility of this screening assay.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Immunology
Vanna Imsirovic, Maja Lenartic, Felix M. M. Wensveen, Bojan Polic, Vedrana Jelencic
Summary: Natural killer (NK) cells, important in early defense against tumors and virally infected cells, are regulated by a balance of activating and inhibitory receptors. However, some activating receptors lack an inhibitory partner. This study shows that activating NK cell receptors without inhibitory partners are not a "master" signal, but instead their activity is controlled through regulation of other activating receptors. This finding has potential implications for the development of NK cell-based cancer immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Neda K. Dezfuli, Shamila D. Alipoor, Neda Dalil Roofchayee, Sharareh Seyfi, Babak Salimi, Ian M. Adcock, Esmaeil Mortaz
Summary: The study found that miR-146a expression was significantly downregulated in NSCLC patients, while IL-6 and TGF-beta levels were elevated. The frequencies of immune cells CD3+ CTLA-4+ and Treg cells were also higher in patients.
FRONTIERS IN ONCOLOGY
(2021)
Article
Immunology
Xinghui Xiao, Ying Cheng, Xiaodong Zheng, Yuhang Fang, Yu Zhang, Rui Sun, Zhigang Tian, Haoyu Sun
Summary: Bispecific antibodies that target CD3 have been widely used in tumor treatment, but may cause severe side effects. Our study developed two IgG-like bispecific antibodies, BK1 and BT1, which targeted NK cells and T cells, respectively. It was found that BK1 had a stronger antitumor effect and induced fewer proinflammatory cytokines compared to BT1. Combining BK1 with BT1 further reduced cytokine secretion by T cells, suggesting a promising future for NK-cell engagers in clinical settings.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
LiangYu Zhao, Sha Han, HengChuan Su, JianYing Li, ErLei Zhi, Peng Li, ChenCheng Yao, RuHui Tian, HuiXing Chen, HuiRong Chen, JiaQiang Luo, ChenKun Shi, ZhiYong Ji, JianLin Hu, Gang Wu, WeiDong Zhou, YuXin Tang, YuZhuo Chen, GuiTing Lin, Tom F. Lue, DengLong Wu, Zheng Li
Summary: This study provides insights into the cellular heterogeneity and signalling networks of the human corpus cavernosum. It describes the spatial distribution of different cell types and identifies potential regulatory signalling pathways in erectile dysfunction.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Kul Raj Rai, Yuan Liao, Mengjuan Cai, Haori Qiu, Faxin Wen, Min Peng, Song Wang, Shasha Liu, Guijie Guo, Xiaojuan Chi, Mohamed Maarouf, Yuhai Chen, Shile Huang, Ji-Long Chen
Summary: In this study, lncRNA-155KO mice lacking most of the lncRNA-155 sequences were found to be more susceptible to influenza virus or pseudorabies virus infection compared to miRNA-155KO mice. The loss of lncRNA-155 resulted in faster body weight loss, poorer survival, and higher viral load. The findings suggest the involvement of lncRNA-155 in regulating viral pathogenesis in addition to miRNA-155. miRNA-155-deleted lncRNA-155 significantly attenuated the viral infection.
Article
Biochemistry & Molecular Biology
Xueping Lai, Qin Chen, Changhong Zhu, Rong Deng, Xian Zhao, Cheng Chen, Yanli Wang, Jianxiu Yu, Jian Huang
Article
Biochemistry & Molecular Biology
Changhong Zhu, Cheng Chen, Jian Huang, Hailong Zhang, Xian Zhao, Rong Deng, Jinzhuo Dou, Hui Jin, Ran Chen, Ming Xu, Qin Chen, Yanli Wang, Jianxiu Yu
NUCLEIC ACIDS RESEARCH
(2015)
Article
Multidisciplinary Sciences
Cheng Chen, Changhong Zhu, Jian Huang, Xian Zhao, Rong Deng, Hailong Zhang, Jinzhuo Dou, Qin Chen, Ming Xu, Haihua Yuan, Yanli Wang, Jianxiu Yu
NATURE COMMUNICATIONS
(2015)
Article
Oncology
Rong Deng, Xian Zhao, YingYing Qu, Cheng Chen, Changhong Zhu, Hailong Zhang, Haihua Yuan, Hui Jin, Xin Liu, Yanli Wang, Qin Chen, Jian Huang, Jianxiu Yu
Review
Microbiology
Bolong Liu, Xin Liu, Shao-Jun Tang
FRONTIERS IN MICROBIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Qin Chen, Xian Zhao, Hailong Zhang, Haihua Yuan, Miaojun Zhu, Qian Sun, Xueping Lai, Yanli Wang, Jian Huang, Jianshe Yan, Jianxiu Yu
MOLECULAR CARCINOGENESIS
(2015)
Article
Neurosciences
Wenjuan Ru, Xin Liu, Chilman Bae, Yuqiang Shi, Randall Walikonis, Jin Mo Chung, Shao-Jun Tang
JOURNAL OF NEUROSCIENCE
(2019)
Article
Multidisciplinary Sciences
Junying Zheng, Wenjuan Ru, Jay R. Adolacion, Michael S. Spurgat, Xin Liu, Subo Yuan, Rommel X. Liang, Jianli Dong, Andrew S. Potter, S. Steven Potter, Ken Chen, Rui Chen, Navin Varadarajan, Shao-Jun Tang
Summary: The study revealed overlapping but distinct microglial populations in the cortex and the spinal cord, with varying responses to life span progression and HIV-1 pathogenic protein gp120. This suggests that microglia in different CNS compartments are adapted to fulfill region-specific biological functions in their local environments.
Article
Anesthesiology
Xin Liu, Bo-Long Liu, Qing Yang, Xiangfu Zhou, Shao-Jun Tang
Summary: Long-term use of opioid analgesics leads to paradoxical pain, where microglia were previously believed to play a critical role. However, experiments showed that ablating microglia did not cause detectable impairment in the expression of hyperalgesia induced by opioids.
Article
Clinical Neurology
Xin Liu, Chilman Bae, Benjamin B. Gelman, Jin Mo Chung, Shao-Jun Tang
Summary: This article investigates the pathogenesis of HIV-associated pain and reveals the crucial role of astrogliosis in pain development. The authors demonstrate that astrogliosis is controlled by a neuron-to-astrocyte Wnt5a signal, and its disruption blocks mechanical hyperalgesia and neural circuit polarization induced by the HIV protein gp120. They also uncover that the astrogliosis promotes hyperalgesia and neuropathic circuit polarization through IL-1beta regulated by the Wnt5a-ROR2-MMP2 axis.
Article
Neurosciences
Austin Kearns, Jazmine Jayasi, Xin Liu, Jigong Wang, Yuqiang Shi, Jin Mo Chung, Jun-Ho La, Shao-Jun Tang, Chilman Bae
Summary: Repeated morphine treatment alters synaptic strengths in specific cell types in the mouse spinal dorsal horn, leading to enhanced pain sensitivity. This mechanism may provide a potential therapeutic target for preventing or treating opioid-induced pain.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Xin Liu, Chilman Bae, Bolong Liu, Yong-Mei Zhang, Xiangfu Zhou, Donghang Zhang, Cheng Zhou, Adriana DiBua, Livia Schutz, Martin Kaczocha, Michelino Puopolo, Terry P. Yamaguchi, Jin Mo Chung, Shao-Jun Tang
Summary: This study reveals the importance of morphine-induced astrogliosis in the development of opioid-induced hyperalgesia (OIH) and elucidates a neuron-to-astrocyte intercellular Wnt signaling pathway that controls astrogliosis.
MOLECULAR PSYCHIATRY
(2023)
Article
Biochemistry & Molecular Biology
Yanmin Guo, Jianfeng He, Hailong Zhang, Ran Chen, Lian Li, Xiaojia Liu, Caihu Huang, Zhe Qiang, Zihan Zhou, Yanli Wang, Jian Huang, Xian Zhao, Junke Zheng, Guo-Qiang Chen, Jianxiu Yu
Summary: This study reveals the involvement of PTEN M1-ubiquitination in the regulation of AKT signaling and prostate cancer progression. The researchers identified specific ubiquitination sites on PTEN and showed that the M1-ubiquitination level correlates with clinical outcomes in prostate cancer patients. Additionally, they discovered that HOIP, a component of the LUBAC complex, plays a role in enhancing AKT signaling in a PTEN-dependent manner.
Article
Biochemistry & Molecular Biology
Jiayu Fang, Yuqin Zhang, Caihu Huang, Runhui Lu, Jie Yu, Ran Chen, Yanli Wang, Xian Zhao, Jianxiu Yu, Jian Huang
Summary: Glycosylation modification regulates the biological function of RPTP alpha and promotes tumor development.
Article
Neurosciences
Mengchan Ou, Yali Chen, Jin Liu, Donghang Zhang, Yaoxin Yang, Jiefei Shen, Changhong Miao, Shao-Jun Tang, Xin Liu, Daniel K. Mulkey, Tao Zhu, Cheng Zhou
Summary: Astrocyte activation in the spinal dorsal horn may contribute to chronic neuropathic pain. The Kir4.1 channel in astrocytes is important for pain regulation, but its regulation and role in behavioral hyperalgesia remain unclear. This study found that Kir4.1 expression was decreased in spinal astrocytes after chronic constriction injury, and conditional knockout of Kir4.1 led to hyperalgesia while overexpression relieved pain. Kir4.1 expression was regulated by MeCP2. Knockdown of Kir4.1 increased astrocyte excitability and altered neuronal firing patterns in the spinal cord. Targeting spinal Kir4.1 could be a therapeutic approach for hyperalgesia in chronic neuropathic pain.
PROGRESS IN NEUROBIOLOGY
(2023)