期刊
IMMUNOGENETICS
卷 62, 期 11-12, 页码 711-719出版社
SPRINGER
DOI: 10.1007/s00251-010-0470-z
关键词
HLA; RT-PCR; Microdissection; Normal tissues; Gene expression
资金
- Fondo de Investigaciones Sanitarias [08/1265]
- Red Genomica del Cancer (RETICRD) [06/020]
- Consejeria de Salud Junta de Andalucia, Proyecto de Investigacion de Excelencia [CTS-3952, CVI-4740]
- Plan Andaluz de Investigacion (PAI) in Spain [CTS-143]
- European Searchable Tumour Cell Line Database (ESTDAB) [QLRI-CT-2001-01325]
- European Network (European community) [LSHC-CT-2004-503306]
- European community [OJ 2004/c158,18234]
We developed a novel human leukocyte antigen HLA-ABC locus-specific quantitative real-time polymerase chain reaction (PCR) to determine the locus-specific gene expression of HLA-ABC in peripheral blood leukocytes (PBLs, n = 53), colon mucosa (n = 15), and larynx mucosa (n = 15). Laser-assisted tissue microdissection allowed us to study the selected cells without interference from surrounding stroma. We report evidence on the specificity of the technique, describing the HLA-ABC locus-specific gene expression patterns found in the PBLs and two solid tissues studied. PBLs showed a higher gene expression of HLA-B than of HLA-A or HLA-C (p = 4.7 x 10(-10) and p = 1.6 x 10(-6), respectively). In solid tissue, HLA-A and HLA-B gene expressions were similar and HLA-C expression lower. In particular, in larynx mucosa, significant differences were found between HLA-A and HLA-C expressions and between HLA-B and HLA-C expressions (p = 6.5 x 10(-4) and p = 8.1 x 10(-4), respectively). The same differences were observed in colon mucosa, but significance was not reached (p = 0.08 and p = 0.06, respectively). Differences in locus-specific regulation may be related to the control of cytotoxic responses of NK and CD8 positive T cells. Gene expression of HLA-ABC specific locus showed no intra-individual variability, but there was a high inter-individual variability. This may result from differences in the expression of common regulatory factors that control HLA-ABC constitutive expression.
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