期刊
IMMUNOBIOLOGY
卷 215, 期 9-10, 页码 804-811出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.imbio.2010.05.015
关键词
Tuberculosis; Innate immunity; NOD-like receptor; Interleukin-18; Granuloma
类别
资金
- Cluster of Excellence Inflammation at Interfaces (Borstel-Kiel-Lubeck-Plon) [EXC306]
In vitro, Toll-like receptors (TLR)2, 4 and 9 as well as NOD-like receptor 2 critically determine macrophage responses to Mycobacterium tuberculosis (Mtb) infection. However, in low-dose experimental murine tuberculosis, single or multiple deficiencies in TLRs 2, 4, 9 or NOD2 have little, if any, impact on early mycobacterial growth containment, granuloma formation and survival. Here, we analyzed the relevance of NALP3, one component of the danger-signaling inflammasome, for (i) Mtb-induced cytokine secretion in vitro and in vivo, (ii) restriction of Mtb replication in infected organs and (iii) granuloma formation. In the absence of functional NALP3, there was no IL-1 beta and IL-18 production in Mtb-infected dendritic cells and macrophages in vitro, whereas secretion of IL-1 alpha, IL-12p40 and TNF remained unaffected. After three weeks of infection, NALP3-deficient as well as IL-18-deficient mice were as capable as wildtype mice of restricting Mtb loads at a plateau level within well-differentiated granulomas. In conclusion, despite its involvement in cytokine processing, NALP3 is not essential for induction of protective immunity to Mtb. (C) 2010 Elsevier GmbH. All rights reserved.
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