Article
Toxicology
Sarah McCarrick, Valentin Romanovski, Zheng Wei, Elin M. Westin, Kjell-Arne Persson, Klara Trydell, Richard Wagner, Inger Odnevall, Yolanda S. Hedberg, Hanna L. Karlsson
Summary: The study demonstrates that welding particles generated using optimized Cr(VI)-reduced wires are less toxic compared to standard welding wires, showing reduced cytotoxicity and no DNA damage. The release of Cr(VI) from standard wires in cell media seemed to explain most of the cytotoxicity and DNA damage. Different welding particles caused similar inflammatory effects, suggesting different underlying mechanisms.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Hematology
Max Yano, Chia Sharpe, J. Rachel Lance, Janani Ravikrishnan, Kevan Zapolnik, Xiaokui Mo, Jennifer A. Woyach, Deepa Sampath, Adam S. Kittai, Sumithira Vasu, Seema Bhat, Kerry A. Rogers, Dean A. Lee, Natarajan Muthusamy, John C. Byrd
Summary: Stimulation with feeder cells expressing mbIL-21 can rapidly expand both healthy donor-derived and CLL patient-derived NK cells, which exhibit potent cytotoxic activity against CLL cells. Combination with anti-CD20 antibodies greatly enhances NK recognition and killing of CLL targets. The use of mbIL-21-expanded NK cells in combination with obinutuzumab and venetoclax shows efficacy in xenograft mouse models of CLL.
Article
Metallurgy & Metallurgical Engineering
Elin M. Westin, S. McCarrick, L. Laundry-Mottiar, Z. Wei, M. C. Biesinger, I Barker, R. Wagner, K-A Persson, K. Trydell, I Odnevall, H. L. Karlsson, Y. S. Hedberg
Summary: Welding fumes, especially those from stainless steel welding, have been found to be carcinogenic due to the increased formation of hexavalent chromium. Austenitic stainless steel flux-cored wires of 316L type have been developed to reduce the toxicity of the welding fume without compromising usability. Various analytical techniques were used to characterize the particles and assess their toxicity, showing that the new optimized 316L-type flux-cored wires generated less Cr(VI) and were less toxic compared to standard flux-cored wires.
WELDING IN THE WORLD
(2021)
Article
Immunology
Hamidreza Ebrahimiyan, Amirhossein Tamimi, Bahareh Shokoohian, Neda Minaei, Arash Memarnejadian, Nikoo Hossein-Khannazer, Moustapha Hassan, Massoud Vosough
Summary: CAR-NK cells offer a promising alternative to CAR-T cell therapy in cancer immunotherapy. They have advantages such as lack of side effects, GvHD, and diverse cell sources, making CAR-NK therapy more feasible.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Medicine, General & Internal
Lenka Koklesova, Alena Liskova, Marek Samec, Tawar Qaradakhi, Anthony Zulli, Karel Smejkal, Karol Kajo, Jana Jakubikova, Payam Behzadi, Martin Pec, Pavol Zubor, Kamil Biringer, Taeg Kyu Kwon, Dietrich Busselberg, Gustavo R. Sarria, Frank A. Giordano, Olga Golubnitschaja, Peter Kubatka
Article
Oncology
Michal Mego, Katarina Kalavska, Marian Karaba, Gabriel Minarik, Juraj Benca, Tatiana Sedlackova, Paulina Gronesova, Dana Cholujova, Daniel Pindak, Jozef Mardiak, Peter Celec
Article
Biochemistry & Molecular Biology
Bozena Smolkova, Zuzana Cierna, Katarina Kalavska, Svetlana Miklikova, Jana Plava, Gabriel Minarik, Tatiana Sedlackova, Dana Cholujova, Paulina Gronesova, Marina Cihova, Karolina Majerova, Marian Karaba, Juraj Benca, Daniel Pindak, Jozef Mardiak, Michal Mego
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Zuzana Cierna, Bozena Smolkova, Dana Cholujova, Paulina Gronesova, Svetlana Miklikova, Marina Cihova, Jana Plava, Michal Mego
Summary: This study found that high PD-L1 expression in breast cancer patients is associated with shorter progression-free survival, as well as low circulating levels of VEGF, TNF-beta and IL-15. Multivariate analysis showed that patients with low VEGF levels had an increased risk of PD-L1 overexpression. Plasma cytokines could potentially serve as non-invasive biomarkers for identifying high-risk breast cancer cases.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Erika Dutkova, Zdenka Lukacova Bujnakova, Oleh Sphotyuk, Jana Jakubikova, Danka Cholujova, Viera Siskova, Nina Daneu, Matej Balaz, Jaroslav Kovac, Jaroslav Kovac, Jaroslav Briancin, Pavlo Demchenko
Summary: In this study, CuInSe2/ZnS multiparticulate nanocomposites were synthesized using a two-step mechanochemical synthesis method. The stability of the prepared nanosuspensions was effectively achieved through sodium dodecyl sulphate capping, as confirmed by zeta potential measurements.
Article
Biochemical Research Methods
Matus Durdik, Pavol Kosik, Lukas Jakl, Maria Kozackova, Eva Markova, Katarina Vigasova, Katarina Beresova, Jana Jakubikova, Eva Horvathova, Lucian Zastko, Marta Fekete, Ingrid Zavacka, Margita Pobijakova, Igor Belyaev
Summary: The study compared the use of imaging flow cytometry (IFC) and fluorescence microscopy (FM) in analyzing DNA double strand breaks induced by ionizing radiation, finding that IFC was more effective in detecting pan-staining. It was also discovered that pan-staining may be associated with early stages of the apoptotic process.
Article
Multidisciplinary Sciences
Csaba Adori, Teresa Daraio, Raoul Kuiper, Swapnali Barde, Lubica Horvathova, Takashi Yoshitake, Robert Ihnatko, Ismael Valladolid-Acebes, Pauline Vercruysse, Ashley M. Wellendorf, Roberto Gramignoli, Bela Bozoky, Jan Kehr, Elvar Theodorsson, Jose A. Cancelas, Boris Mravec, Carl Jorns, Ewa Ellis, Jan Mulder, Mathias Uhlen, Christina Bark, Tomas Hokfelt
Summary: Research found that the hepatic nerves in NAFLD pathology show signs of degeneration, with chronic sympathetic hyperexcitation being a key factor in axonal degeneration. In experimental steatohepatitis, severe portal vein contraction was observed, spatially correlated with the extension of remaining nerves around the portal vein, providing insight into a potential intrahepatic neuronal mechanism of portal hypertension.
Article
Medicine, General & Internal
Daniela Gornyk, Manuela Harries, Stephan Glockner, Monika Strengert, Tobias Kerrinnes, Jana-Kristin Heise, Henrike Maass, Julia Ortmann, Barbora Kessel, Yvonne Kemmling, Berit Lange, Gerard Krause
Summary: This study revealed that the seroprevalence rate of COVID-19 among unvaccinated individuals in various districts in Germany increased from 1.3-2.8% between July and December 2020 to 4.1-13.1% between February and May 2021. The seroprevalence rate showed a downward trend over the course of the pandemic.
DEUTSCHES ARZTEBLATT INTERNATIONAL
(2021)
Article
Oncology
Yi Chieh Lim, Kamilla E. Jensen, Diana Aguilar-Morante, Lina Vardouli, Kristoffer Vitting-Seerup, Ryan C. Gimple, Qiulian Wu, Henriette Pedersen, Kirstine J. Elbaek, Irina Gromova, Robert Ihnatko, Bjarne W. Kristensen, Jeanette K. Petersen, Jane Skjoth-Rasmussen, William Flavahan, Jeremy N. Rich, Petra Hamerlik
Summary: In this study, we identified PFKM as a driver of bevacizumab resistance in GBM. Cytosolic PFKM interacted with KIF11 to promote tumor invasion, while nuclear PFKM safeguarded genomic stability of tumor cells through interaction with NBS1. Based on these findings, we explored the potential of the drug bupivacaine to target PFKM and found that it enhanced the efficacy of bevacizumab in preclinical GBM models in vivo.
Article
Multidisciplinary Sciences
Danka Cholujova, Lenka Koklesova, Zdenka Lukacova Bujnakova, Erika Dutkova, Zuzana Valuskova, Patricia Beblava, Anna Matisova, Jan Sedlak, Jana Jakubikova
Summary: This study evaluated the anti-myeloma activity of three types of nanoparticles in multiple myeloma and found that they induced apoptosis, triggered G(2)/M arrest, and activated myeloma-related signaling pathways. Additionally, these nanoparticles showed synergistic effects with other drugs, suggesting their potential for future clinical studies.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Michal Mego, Barbora Vlkova, Gabriel Minarik, Zuzana Cierna, Marian Karaba, Juraj Benca, Tatiana Sedlackova, Dana Cholujova, Paulina Gronesova, Katarina Kalavska, Daniel Pindak, Jozef Mardiak, Peter Celec
Summary: Circulating tumor cells (CTCs) are significant in breast cancer metastasis, and their presence is an independent predictor of survival. Vitamin D levels were found to be lower in breast cancer patients with CTCs. Plasma vitamin D levels were inversely correlated with TGF-beta 1, TGF-beta 2, IL beta, IL-5, and eotaxin. Higher vitamin D levels were associated with better overall survival.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Danka Cholujova, Gabor Beke, Zachary R. Hunter, Teru Hideshima, Ludmila Flores, Tatiana Zeleznikova, Denisa Harrachova, Lubos Klucar, Merav Leiba, Lubos Drgona, Steven P. Treon, Efstathios Kastritis, David M. Dorfman, Kenneth C. Anderson, Jana Jakubikova
Summary: By using mass cytometry, this study characterized the immunophenotypic changes in Waldenstrom macroglobulinemia (WM) and revealed the modulation of immune cells by immune checkpoints. It was found that the response to treatment strategies in WM can be monitored by observing the changes in immune cells.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Chemistry, Medicinal
Zdenka Lukacova Bujnakova, Erika Dutkova, Jana Jakubikova, Danka Cholujova, Rastislav Varhac, Larysa Borysenko, Inna Melnyk
Summary: In this paper, ZnS nanoparticles were successfully bioconjugated with bovine serum albumin and prepared as a nanosuspension using wet circulation grinding. The interactions between ZnS and albumin were studied, and the potential application of the nanosuspension in multiple myeloma cells was evaluated. The slight changes in the secondary structure of albumin and the stability of the system were also investigated.
Article
Oncology
L. Jakl, M. Skorvaga, K. Beresova, P. Kosik, M. Durdik, J. Jakubikova, M. Holop, M. Kubes, L. Zastko, E. Markova, I Belyaev
