期刊
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 49, 期 -, 页码 509-516出版社
ELSEVIER
DOI: 10.1016/j.msec.2015.01.002
关键词
Polymer scaffold; 3D; Cell culture; Hepatocyte; Polylactic acid (PLA)
资金
- Federal Ministry of Education and Research
- Thuringian Ministry of Culture and Federal Ministry of Economics within the Initiative Centre for Innovation Competence, MacroNano(R) [FKZ: 09-BIO 03WKCB01O, FKZ 16SV5473, TMK/TMWAI FKZ B714-09064, FKZ 03Z1M511, FKZ KF2731203MD2]
- NPST at King Saud University [09-BIO676-02]
Within the scientific community, there is an increasing demand to apply advanced cell cultivation substrates with increased physiological functionalities for studying spatially defined cellular interactions. Porous polymeric scaffolds are utilized for mimicking an organ-like structure or engineering complex tissues and have become a key element for three-dimensional (3D) cell cultivation in the meantime. As a consequence, efficient 3D scaffold fabrication methods play an important role in modern biotechnology. Here, we present a novel thermoforming procedure for manufacturing porous 3D scaffolds from permeable materials. We address the issue of precise thermoforming of porous polymer foils by using multilayer polymer thermoforming technology. This technology offers a new method for structuring porous polymer foils that are otherwise available for non-porous polymers only. We successfully manufactured 3D scaffolds from solvent casted and phase separated polylactic acid (PLA) foils and investigated their biocompatibility and basic cellular performance. The HepG2 cell culture in PLA scaffold has shown enhanced albumin secretion rate in comparison to a previously reported polycarbonate based scaffold with similar geometry. (C) 2015 Elsevier B.V. All rights reserved.
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