Article
Ophthalmology
David Maskill, Arthur Okonkwo, Charlotte Onsiong, Stella Hristova, Amy Dodd, Seema Anand
Summary: This study compared 10 years of repeat corneal collagen cross-linking (CXL) with primary CXL and found that younger age is associated with failure of primary CXL. Repeat CXL is a safe and effective treatment for progressive keratoconus, even after primary CXL.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Review
Ophthalmology
Mohammad Karam, Abdulmalik Alsaif, Ahmed Aldubaikhi, Meshaal Aljebreen, Rand Alazaz, Nahlaa Alkhowaiter, Tariq Almudhaiyan, Faisal Aljassar
Summary: The aim of this study was to compare the outcomes of 18 mW/cm(2) (5 minutes) versus 9 mW/cm(2) (10 minutes) accelerated corneal collagen cross-linking protocols in patients with progressive keratoconus. The study found that the 10-minute protocol had better outcomes in terms of K1, K2, and HOA compared to the 5-minute protocol, but there were no statistically significant differences in other outcomes.
Article
Biology
Turki Almubrad, Rita Mencucci, Adrian Smedowski, Ramachandran Samivel, Essam Almutleb, Aljoharah Alkanaan, Adnan Ali Khan, Ali Masmali, Saeed Akhtar
Summary: Electron microscopy revealed that iontophoresis-ultraviolet A (UVA) cross-linking (CXL) with hypotonic riboflavin solution preserved collagen fibril diameter and proteoglycan area size better in the peripheral cornea compared to the central cornea. Riboflavin + UVA application at 3 mW/cm^2 for 30 min and 10 mW/cm(2) for 9 min were effective CXL methods, with the former improving collagen fibril organization and size.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Ophthalmology
Atikah Haneef, Ramprasad Obula Giridhara Gopalan, Divya T. Rajendran, Jessica Nunes, Dharmalingam Kuppamuthu, Naveen Radhakrishnan, Tai-Horng Young, Hao-Ying Hsieh, Namperumalsamy Venkatesh Prajna, Colin E. Willoughby, Rachel Williams
Summary: The novel chemical cross-linker treatment for keratoconus involving carbodi-imide chemistry showed clinically relevant increase in corneal stiffness without removal of corneal epithelium. Histochemical analysis indicated no gross morphology changes or apoptosis in porcine and human corneas. In vivo treatment of rabbit eyes resulted in initial corneal epithelium thinning which recovered over time, with irregular cell distribution. The cross-linker demonstrated lower cytotoxicity to stromal cells than traditional ultraviolet A treatment, suggesting potential as a treatment for keratoconus.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Physical
Masako Fujioka-Kobayashi, Elena Andrejova, Hiroki Katagiri, Benoit Schaller, Anton Sculean, Jean-Claude Imber, Niklaus P. Lang, Nikola Saulacic
Summary: The study found that cross-linked collagen matrices may be advantageous for maintaining fibrous tissue volume without disrupting bone formation in defects. The cross-linking of collagen did not significantly impact the potential for bone formation.
Article
Biochemistry & Molecular Biology
Morten M. Jensen, Arkadiusz Bonna, Sigurd J. Frederiksen, Samir W. Hamaia, Peter Hojrup, Richard W. Farndale, Henrik Karring
Summary: This study identifies the presence of Dermatopontin (DPT) in porcine corneal stroma and explores its binding sites on collagens II and III. The binding sites for DPT on triple-helical collagens are found to be located at the crosslinking and collagenase cleavage sites. The results suggest that disrupted DPT binding to fibrillar collagens may contribute to certain connective tissue disorders. These findings provide a foundation for further investigation of the role of DPT.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2022)
Article
Ophthalmology
Daniel F. P. Larkin, Kashfia Chowdhury, Jennifer M. Burr, Mathew Raynor, Matthew Edwards, Stephen J. Tuft, Catey Bunce, Emilia Caverly, Caroline Dore
Summary: The study demonstrates that corneal cross-linking (CXL) can effectively halt the progression of keratoconus in the majority of young patients, positioning it as a first-line treatment for progressive disease and potentially offering the benefit of avoiding later need for contact lens wear or corneal transplantation.
Review
Chemistry, Multidisciplinary
Wei-Hua Han, Qing-Yu Wang, Yuan-Yi Kang, Li-Rui Shi, Yu Long, Xin Zhou, Chun-Cheng Hao
Summary: This review comprehensively discusses the cross-linking electrospinning system for the first time, including cross-linking strategies, cross-linking mechanisms, and applications of cross-linked electrospun nanofibers. The significance of cross-linking electrospinning and the challenges in constructing this system are also highlighted.
Review
Pharmacology & Pharmacy
Promita Bhattacharjee, Mark Ahearne
Summary: This paper provides an overview of different crosslinking techniques and chemical additives used in hydrogels for corneal tissue engineering, drug delivery, or corneal repair. Factors influencing the success of a crosslinker, such as material composition, dosage, fabrication method, immunogenicity, and toxicity, are considered. The choice of crosslinking technique significantly affects the biocompatibility, mechanical properties, and chemical structure of hydrogels suitable for corneal tissue engineering and regenerative applications.
Article
Medicine, General & Internal
Pasquale Napolitano, Fausto Tranfa, Luca D'Andrea, Ciro Caruso, Michele Rinaldi, Alberto Mazzucco, Nicola Ciampa, Antonietta Melenzane, Ciro Costagliola
Summary: This study compared the effectiveness of iontophoresis epi-on and epi-off techniques in corneal collagen cross-linking (CXL) and found that both techniques are valid for mid-term corneal stabilization. The advantage of the iontophoresis epi-off technique lies in a faster imbibing time of the cornea, reducing surgical times and lowering the risk of complications.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Engineering, Biomedical
You Wu, Shoucheng Chen, Pu Luo, Shudan Deng, Zhengjie Shan, Jinghan Fang, Xingchen Liu, Jiaxin Xie, Runheng Liu, Shiyu Wu, Xiayi Wu, Zetao Chen, Kelvin W. K. Yeung, Quan Liu, Zhuofan Chen
Summary: Incorporating zinc-doped nanohydroxyapatite (nZnHA) and cross-linking with alendronate can improve the performance of biogenic collagen membranes, increasing their degradation period and providing good biocompatibility and tissue integration capability.
ACTA BIOMATERIALIA
(2022)
Article
Ophthalmology
Nazan Acar Eser, Ozlem Dikmetas, Sibel Kocabeyoglu, Cagman Tan, Murat Irkec
Summary: Comparing tear cytokine and chemokine levels in keratoconus (KC) patients and controls can help us understand the etiology more clearly and clarify the molecular changes after cross-linking (CXL).
OCULAR IMMUNOLOGY AND INFLAMMATION
(2023)
Article
Polymer Science
Maria Dolores Montalvo-Parra, Wendy Ortega-Lara, Denise Loya-Garcia, Andres Bustamante-Arias, Guillermo-Isaac Guerrero-Ramirez, Cesar E. Calzada-Rodriguez, Guiomar Farid Torres-Guerrero, Betsabe Hernandez-Sedas, Italia Tatnai Cardenas-Rodriguez, Sergio E. Guevara-Quintanilla, Marcelo Salan-Gomez, Miguel Angel Hernandez-Delgado, Salvador Garza-Gonzalez, Mayra G. Gamboa-Quintanilla, Luis Guillermo Villagomez-Valdez, Judith Zavala, Jorge E. Valdez-Garcia
Summary: Corneal opacities, a common cause of visual impairment, are currently treated with corneal transplantation. However, there is a shortage of tissue donors for this procedure. In this study, researchers developed a collagen-based membrane called CVM and used it as a scaffold for corneal cell transplantation. The CVMs showed promising characteristics, such as suitable thickness, transparency, and good cell adherence. Pilot transplantation in rabbits also demonstrated the potential of this technique for corneal transparency restoration.
Review
Cell & Tissue Engineering
Aini Zhang, Wei Zhang, Ludvig J. Backman, Jialin Chen
Summary: Corneal injury is a common problem affecting millions of people worldwide, leading to vision loss and impairment. Current treatment methods, such as corneal transplantation, are limited by the availability of donors. Corneal tissue engineering shows promise as an alternative method, but current experimental strategies have limitations in stem cell differentiation into keratocytes for clinical application. This review aims to clarify the role and effectiveness of biochemical factors, physical regulation, and their combination in inducing stem cell differentiation into keratocytes, as well as propose novel differentiation strategies to improve the efficiency of corneal tissue engineering.
STEM CELLS INTERNATIONAL
(2022)
Article
Chemistry, Multidisciplinary
Xiaoduo Tang, Xiaomeng Wang, Yihan Sun, Liang Zhao, Daowei Li, Junhu Zhang, Hongchen Sun, Bai Yang
Summary: The bio-multifunctional hydrogel CCOD-MgO accelerates wound healing through a double cross-linking mechanism, exhibiting high tissue adhesion, self-repair capabilities, and low swelling rate. In vitro experiments show that CCOD-MgO has higher adhesion strength and antibacterial properties compared to other hydrogels, effectively protecting wounds from infection.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Meng Gao, Luochao Wang, Junjian Chen, Shiwu Li, Guanhai Lu, Lin Wang, Yingjun Wang, Li Ren, Anjun Qin, Ben Zhong Tang
CHEMISTRY-A EUROPEAN JOURNAL
(2016)
Article
Materials Science, Composites
Weichang Li, Junpei Kang, Yulin Yuan, Fengcai Xiao, Hang Yao, Sa Liu, Jianxi Lu, Yingjun Wang, Zhen Wang, Li Ren
COMPOSITES SCIENCE AND TECHNOLOGY
(2016)
Article
Physics, Fluids & Plasmas
Shiheng Yin, Li Ren, Yingjun Wang
PLASMA SCIENCE & TECHNOLOGY
(2017)
Article
Polymer Science
Xuan Zhao, Kai Long, Yang Liu, Weichang Li, Sa Liu, Lin Wang, Li Ren
JOURNAL OF APPLIED POLYMER SCIENCE
(2017)
Article
Chemistry, Medicinal
Shuimu Lin, Jun-Jie Koh, Thet Tun Aung, Fanghui Lim, Jianguo Li, Hanxun Zou, Lin Wang, Rajamani Lakshminarayanan, Chandra Verma, Yingjun Wang, Donald T. H. Tan, Derong Cao, Roger W. Beuerman, Li Ren, Shouping Liu
JOURNAL OF MEDICINAL CHEMISTRY
(2017)
Article
Materials Science, Biomaterials
Hang Yao, Jingchen Xue, Qunfang Wang, Renjian Xie, Weichang Li, Sa Liu, Jinglei Cai, Dajiang Qin, Dong-An Wang, Li Ren
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2017)
Article
Chemistry, Multidisciplinary
Siheng Li, Lin Wang, Fei Yu, Zhiling Zhu, Dema Shobaki, Haoqing Chen, Mu Wang, Jun Wang, Guoting Qin, Uriel J. Erasquin, Li Ren, Yingjun Wang, Chengzhi Cai
Article
Chemistry, Multidisciplinary
Sa Liu, Minglei Chu, Yongjun Zhu, Lifeng Li, Lin Wang, Huichang Gao, Li Ren
Article
Multidisciplinary Sciences
Xuhua Tan, Jiezhao Zhan, Yi Zhu, Ji Cao, Lin Wang, Sa Liu, Yingjun Wang, Zhenzhen Liu, Yingyan Qin, Mingxing Wu, Yizhi Liu, Li Ren
SCIENTIFIC REPORTS
(2017)
Article
Materials Science, Biomaterials
Sa Liu, Min Jin, Yunhua Chen, Huichang Gao, Xuetao Shi, Wenhua Cheng, Li Ren, Yingjun Wang
JOURNAL OF MATERIALS CHEMISTRY B
(2017)
Article
Materials Science, Biomaterials
Sa Liu, Chunting Zhong, Junjian Chen, Jiezhao Zhan, Jingcai He, Yuchen Zhu, Yingjun Wang, Lin Wang, Li Ren
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2017)
Article
Materials Science, Biomaterials
Sa Liu, Chunting Zhong, Junjian Chen, Jiezhao Zhan, Jingcai He, Yuchen Zhu, Yingjun Wang, Lin Wang, Li Ren
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2017)
Article
Biochemistry & Molecular Biology
Kun Zhang, Yong-Guang Jia, I-Huang Tsai, Satu Strandman, Li Ren, Liangzhi Hong, Guangzhao Zhang, Ying Guan, Yongjun Zhang, X. X. Zhu
Article
Chemistry, Physical
Jiongrun Chen, Xuetao Shi, Li Ren, Yingjun Wang
Article
Chemistry, Physical
Yunhua Chen, Yilin Wang, Xuetao Shi, Min Jin, Wenhua Cheng, Li Ren, Yingjun Wang
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)