期刊
IEEE TRANSACTIONS ON NANOBIOSCIENCE
卷 11, 期 1, 页码 28-36出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TNB.2011.2165728
关键词
Cell adhesion; Chinese Hamster Ovary (CHO) cells; microenvironment; nanofabrication; nanogrooves; substrate rigidity; topographical cues
资金
- World Class University (WCU) [R31-2008-000-10083-0]
- Basic Science Research Program [2010-0027955]
- Center for Nanoscale Mechatronics Manufacturing [08 K1401-00710]
- National Institutes of Health [R21-EB008562]
- National Research Foundation of Korea [14-2008-01-001-00, 과C6A1803, R31-2012-000-10083-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Live cells are exquisitely sensitive to both the substratum rigidity and texture. To explore cell responses to both these types of inputs in a precisely controlled fashion, we analyzed the responses of Chinese hamster ovary (CHO) cells to nanotopographically defined substrata of different rigidities, ranging from 1.8 MPa to 1.1 GPa. Parallel arrays of nanogrooves (800-nm width, 800-nm space, and 800-nm depth) on polyurethane (PU)-based material surfaces were fabricated by UV-assisted capillary force lithography (CFL) over an area of 5 mm x 3 mm. We observed dramatic morphological responses of CHO cells, evident in their elongation and polarization along the nanogrooves direction. The cells were progressively more spread and elongated as the substratum rigidity increased, in an integrin beta 1 dependent manner. However, the degree of orientation was independent of substratum rigidity, suggesting that the cell shape is primarily determined by the topographical cues.
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