4.5 Article

Characterizing Mechanical Properties of Biological Cells by Microinjection

期刊

IEEE TRANSACTIONS ON NANOBIOSCIENCE
卷 9, 期 3, 页码 171-180

出版社

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TNB.2010.2050598

关键词

Biological cells; constitutive material; mechanical model; mechanical properties; microinjection

资金

  1. Research Grants Council of the Hong Kong Special Administrative Region, China [CityU 120308]
  2. City University of Hong Kong [9360131]

向作者/读者索取更多资源

Microinjection has been demonstrated to be an effective technique to introduce foreign materials into biological cells. Despite the advance, whether cell injection can be used to characterize the mechanical properties of cells remains elusive. In this paper, extending the previously developed mechanical model, various constitutive materials are adopted to present the membrane characteristics of cells. To demonstrate the modeling approach and identify the most appropriate constitutive material for a specific biomembrane, finite element analysis (FEA) and experimental tests are carried out. It is shown that the modeling results agree well with those from both FEA and experiments, which demonstrates the validity of the developed approach. Moreover, Yeoh and Cheng materials are found to be the best constitutive materials in representing the deformation behaviors of zebrafish embryos and mouse embryos (or oocytes), respectively. Also, the mechanical properties of zebrafish embryos at different developmental stages and mouse embryos (or oocytes) are characterized.

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