Article
Biotechnology & Applied Microbiology
Pei-Ling Yu, James C. Fulton, Owen H. Hudson, Jose C. Huguet-Tapia, Jeremy T. Brawner
Summary: This study developed a broad-spectrum fungal identification tool using high-throughput Nanopore sequencing and enrichment probes. It provides a fast and accurate method for fungal identification, and also offers a platform for identifying other plant pathogens.
Article
Clinical Neurology
Ying Huang, Yulu Liu, Yongguang Liu, Qiang Li, Xuejun Fu, Liangyu Zou, Qianhui Xu
Summary: A 52-year-old HIV-negative woman presented with bilateral painless central vision loss. NGS analysis of the CSF revealed the presence of Treponema and other pathological features related to visual impairment. After appropriate treatment, her vision improved.
Article
Chemistry, Multidisciplinary
Na Zhao, Jiabao Cao, Jiayue Xu, Beibei Liu, Bin Liu, Dingqiang Chen, Binbin Xia, Liang Chen, Wenhui Zhang, Yuqing Zhang, Xuan Zhang, Zhimei Duan, Kaifei Wang, Fei Xie, Kun Xiao, Wei Yan, Lixin Xie, Hongwei Zhou, Jun Wang
Summary: Utilizing RNA sequencing and third-generation sequencing technology for pathogen identification can improve the ratio of microbial reads and accelerate clinical diagnosis, showing significant potential.
Article
Evolutionary Biology
Lidia Escoda, Jose Castresana
Summary: The Pyrenean desman is a unique semi-aquatic mammal with strong genetic structure due to isolation and environmental factors. Genomic studies reveal its distinctive characteristics and genetic health status.
EVOLUTIONARY APPLICATIONS
(2021)
Article
Nutrition & Dietetics
Hun Hwan Kim, Sang Eun Ha, Min Yeong Park, Se Hyo Jeong, Pritam Bhagwan Bhosale, Abuyaseer Abusaliya, Chung Kil Won, Jeong Doo Heo, Meejung Ahn, Je Kyung Seong, Hyun Wook Kim, Gon Sup Kim
Summary: Using RNA sequencing, this study identified KYNA as a potential therapeutic target for gastric cancer. KYNA treatment inhibited cancer cell growth and proliferation, while inducing cell death. This research can provide valuable insights for the development of drugs targeting gastric cancer.
Article
Genetics & Heredity
Alesa Kristan, Tadej Pajic, Ales Maver, Tadeja Rezen, Tanja Kunej, Rok Kolic, Andrej Vuga, Martina Fink, Spela Zula, Helena Podgornik, Sasa Anzej Doma, Irena Preloznik Zupan, Damjana Rozman, Natasa Debeljak
Summary: An erythrocytosis is characterized by an increase in red blood cell mass, often associated with genetic variants in genes related to oxygen sensing, hematopoietic cell maturation, and oxygen transfer. Classical sequencing approaches may only provide a correct diagnosis for a fraction of patients with congenital erythrocytosis, as the genetic background of the disorder is more complex and involves additional genes. A targeted next-generation sequencing approach was used in this study to identify genetic variants in patients with unexplained erythrocytosis, leading to the discovery of pathogenic and novel variants in relevant genes, as well as the identification of potential associations with iron metabolism genes. This study contributes to improving the diagnosis and management of Slovenian patients with erythrocytosis and provides insights for future research into the etiology of this rare hematological disorder.
FRONTIERS IN GENETICS
(2021)
Article
Virology
Vladimir Loukachov, Karel A. van Dort, Louis Jansen, Henk W. Reesink, Neeltje A. Kootstra
Summary: A novel Hepatitis B Virus (HBV) encoded miRNA, HBV-miR-6, was identified and validated in liver tissue from chronic hepatitis B (CHB) patients. Its expression was correlated with hepatic HBV-DNA and plasma HBsAg levels, suggesting a potential role in viral excretion or particle formation.
Article
Medical Laboratory Technology
Xiaohua Fang, Chaofeng Zhu, Xiaofan Zhu, Yin Feng, Zhihui Jiao, Huikun Duan, Xiangdong Kong, Ning Liu
Summary: This study evaluated gene variations in Chinese MPS patients using targeted NGS technology and identified 11 novel variants associated with disease. MPS II was found to be the most common type in China. The study expands the variation spectrum of MPS, which is important for disease management and genetic counseling.
CLINICA CHIMICA ACTA
(2022)
Article
Medicine, Legal
Shubham Yadav, Vijay Tripathi, Vaibhav Saran
Summary: Characterization of human saliva through Next-Generation Sequencing has revealed the influence of habits, such as smoking, alcohol consumption, and vegan diet, on the microbial composition and diversity of the salivary microbiome.
FORENSIC SCIENCE INTERNATIONAL
(2023)
Article
Genetics & Heredity
Gang Liu, Shumiao Zhang, Xinsheng Zhao, Chao Li, Minghao Gong
Summary: Diet analysis is crucial in animal ecology, and the next generation sequencing (NGS) approach has been suggested as a promising tool to improve efficiency. However, challenges in generating NGS-based diet data and subsequent analyses have hindered its widespread application in the field. More developments and incorporation of additional perspectives are needed to encourage ecologists to gain novel insights in their work.
Article
Oncology
Tyler Shugg, Reynold C. Ly, Wilberforce Osei, Elizabeth J. Rowe, Caitlin A. Granfield, Ty C. Lynnes, Elizabeth B. Medeiros, Jennelle C. Hodge, Amy M. Breman, Bryan P. Schneider, S. Cenk Sahinalp, Ibrahim Numanagic, Benjamin A. Salisbury, Steven M. Bray, Ryan Ratcliff, Todd C. Skaar
Summary: This study assessed the accuracy of the Aldy computational method in extracting pharmacogenomics genotypes from whole genome sequencing (WGS) and whole exome sequencing (WES) data. The results showed that Aldy achieved over 99% accuracy in calling diplotypes for major pharmacogenes from both WGS and WES data, supporting its importance in clinical applications.
FRONTIERS IN ONCOLOGY
(2023)
Article
Microbiology
Ilayda Akacin, Seymanur Ersoy, Osman Doluca, Mine Gungormusler
Summary: This review provides a comprehensive overview of recent literature on the utilization of TGS and NGS technologies in microbial metagenomics research. It discusses the advantages and limitations of these technologies and presents real-time examples of novel applications in clinical microbiology and public health, food and agriculture, energy and environment, arts and space.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Gastroenterology & Hepatology
Yipeng Ma, Jiayu Ou, Tong Lin, Lei Chen, Junhui Chen, Mingjun Wang
Summary: This study presents an HBV-specific TCR identification method that does not require the TCR singularization process, using ranking-based and alpha-beta chain mixture-based strategies. These strategies are effective for identifying HBV-specific TCRs and may be used for treating HBV-related HCC.
HEPATOLOGY COMMUNICATIONS
(2021)
Article
Endocrinology & Metabolism
Sanghoo Lee, Seol-A Kim, Jeonghoon Hong, Yejin Kim, Gayeon Hong, SaeYun Baik, Kyeonghwan Choi, Mi-Kyeong Lee, Kyoung-Ryul Lee
Summary: This study used targeted next-generation sequencing to identify genetic variants related to different clinical characteristics of Metabolic syndrome (MetS), such as obesity, blood glucose, dyslipidemia, and hypertension.
DIABETOLOGY & METABOLIC SYNDROME
(2022)
Article
Microbiology
Chun-Liang Wang, Lan-Yue Zhang, Xin-Yuan Ding, Yi-Cheng Sun
Summary: A next-generation sequencing approach was used to identify mycobacteriophage-encoded proteins that are toxic to Mycobacterium tuberculosis. This high-throughput screening method was efficient and time-saving, and could potentially aid in the discovery of novel antimicrobial molecules.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
R. Leblois, M. Gautier, A. Rohfritsch, J. Foucaud, C. Burban, M. Galan, A. Loiseau, L. Saune, M. Branco, K. Gharbi, R. Vitalis, C. Kerdelhue
Article
Parasitology
A. Dubois, G. Castel, S. Murri, C. Pulido, J. -B. Pons, L. Benoit, A. Loiseau, L. Lakhdar, M. Galan, P. Marianneau, N. Charbonnel
Article
Cell Biology
T. Goeury, L. E. Creary, L. Brunet, M. Galan, M. Pasquier, B. Kervaire, A. Langaney, J. -M. Tiercy, M. A. Fernandez-Vina, J. M. Nunes, A. Sanchez-Mazas
Article
Biotechnology & Applied Microbiology
Ahmadou Sow, Thierry Brevault, Gerard Delvare, Julien Haran, Laure Benoit, Armelle Coeur d'acier, Maxime Galan, Cheikh Thiaw, Valerie Soti, Mbacke Sembene
BIOLOGICAL CONTROL
(2018)
Article
Endocrinology & Metabolism
Celia Bidu, Quentin Escoula, Sandrine Bellenger, Ayme Spor, Maxime Galan, Audrey Geissler, Andre Bouchot, Dominique Dardevet, Beatrice Morio-Liondor, Patrice D. Cani, Laurent Lagrost, Michel Narce, Jerome Bellenger
Article
Biochemistry & Molecular Biology
Maxime Galan, Jean-Baptiste Pons, Orianne Tournayre, Eric Pierre, Maxime Leuchtmann, Dominique Pontier, Nathalie Charbonnel
MOLECULAR ECOLOGY RESOURCES
(2018)
Correction
Endocrinology & Metabolism
Celia Bidu, Quentin Escoula, Sandrine Bellenger, Ayme Spor, Maxime Galan, Audrey Geissler, Andre Bouchot, Dominique Dardevet, Beatrice Morio-Liondor, Patrice D. Cani, Laurent Lagrost, Michel Narce, Jerome Bellenger
Article
Geriatrics & Gerontology
Emmanuel Guivier, Francois Criscuolo, Sandrine Zahn, Jerome Bellenger, Maxime Galan, Bruno Faivre, Gabriele Sorci
EXPERIMENTAL GERONTOLOGY
(2018)
Article
Multidisciplinary Sciences
Marie-Amelie Forin-Wiart, Marie-Lazarine Poulle, Sylvain Piry, Jean-Francois Cosson, Claire Larose, Maxime Galan
SCIENTIFIC REPORTS
(2018)
Article
Ecology
Audrey Rohfritsch, Maxime Galan, Mathieu Gautier, Karim Gharbi, Gert Olsson, Bernhard Gschloessl, Caroline Zeimes, Sophie VanWambeke, Renaud Vitalis, Nathalie Charbonnel
ECOLOGY AND EVOLUTION
(2018)
Article
Ecology
Elodie Portanier, Mathieu Garel, Sebastien Devillard, Daniel Maillard, Jocelyn Poissant, Maxime Galan, Slimania Benabed, Marie-Therese Poirel, Jeanne Duhayer, Christian Itty, Gilles Bourgoin
Article
Microbiology
Jean-Claude Ogier, Sylvie Pages, Maxime Galan, Matthieu Barret, Sophie Gaudriault
Article
Entomology
Ahmadou Sow, Julien Haran, Laure Benoit, Maxime Galan, Thierry Brevault
Article
Microbiology
Johann Vulin, Severine Murri, Sarah Madrieres, Maxime Galan, Caroline Tatard, Sylvain Piry, Gabriele Vaccari, Claudia D'Agostino, Nathalie Charbonnel, Guillaume Castel, Philippe Marianneau
Summary: The study describes the isolation of two strains of PUUMALA orthohantavirus from two distinct French geographical areas: Ardennes (endemic area) and Loiret (non-endemic area). By selecting wild bank voles, cultivating the strains efficiently in Vero E6 cells, and propagating them in immunologically neutral bank voles, the impact of isolation methods on viral diversity was better assessed through high-throughput and Sanger sequencing results.
Article
Multidisciplinary Sciences
Ahmadou Sow, Thierry Brevault, Laure Benoit, Marie-Pierre Chapuis, Maxime Galan, Armelle Coeur d'acier, Gerard Delvare, Mbacke Sembene, Julien Haran
SCIENTIFIC REPORTS
(2019)