Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Maud Savonnet, Mathilde Aubret, Patricia Laurent, Yoann Roupioz, Myriam Cubizolles, Arnaud Buhot
Summary: This paper presents a theoretical framework to analyze the kinetics of the second stage of LAMP, which can help optimize LAMP performance by reducing the number of experiments needed to find the best parameters.
Article
Infectious Diseases
Anna-Clara Ivarsson, Elin Fransen, Ioanna Broumou, Anna Farnert, Kristina E. M. Persson, Sara Karlsson Sobirk
Summary: This study compared the diagnostic performance of two LAMP kits for malaria detection and found that the HumaTurb Loopamp Malaria PDT kit outperformed the Alethia Illumigene Malaria kit.
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Chemistry, Analytical
Qiuyuan Lin, Zhipeng Huang, Xin Ye, Bin Yang, Xueen Fang, Baohong Liu, Hui Chen, Jilie Kong
Summary: This study proposed a lab in a tube system for the detection of exosomal nucleic acids, which integrated exosomes enrichment using IMB, fast exosomes lysis based on NP-40 lysate, and sensitive LAMP. The method successfully detected two long noncoding RNA biomarkers of gastric cancer and exhibited good performance in analyzing exosomal HOTTIP RNA directly in human serum samples, showing potential for detection of low-abundance exosomal nucleic acid biomarkers from cancers.
Article
Multidisciplinary Sciences
Gaolian Xu, Julien Reboud, Yunfei Guo, Hao Yang, Hongchen Gu, Chunhai Fan, Xiaohua Qian, Jonathan M. Cooper
Summary: Accelerating the design of nucleic acid amplification methods is challenging, but necessary for diagnosing diseases. This study presents a generalizable isothermal nucleic acid amplification methodology that can be applied to clinical settings, allowing for the detection of short miRNAs and difficult pathogenic targets.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Article
Multidisciplinary Sciences
Aldrik H. Velders, Michel Ossendrijver, Bart J. F. Keijser, Vittorio Saggiomo
Summary: A solution to the bottlenecks of centralized testing during the pandemic is presented, with a cheap and easy-to-produce noninstrumental device for at-home SARS-CoV2 RNA detection. This device, capable of maintaining isothermal conditions for LAMP tests, has minimal environmental impact and can be deployed on a large scale in a short amount of time.
Review
Biotechnology & Applied Microbiology
Alangar I. Bhat, Rashid Aman, Magdy Mahfouz
Summary: Plant diseases caused by viruses have a significant impact on crop production and quality, resulting in substantial losses. The options for managing viruses are limited due to their nature as systemic obligate parasites. In this regard, sensitive, robust, and affordable diagnostic assays are needed to detect the presence of viruses in plant materials and prevent their spread. Traditional methods based on biological and physical properties have limitations in terms of sensitivity and time consumption. On the other hand, assays based on viral proteins and nucleic acids offer more specificity, sensitivity, and rapidity. However, they often require sophisticated equipment and technical skills in laboratory settings. Isothermal-based assays, such as LAMP and RPA, provide a simple and reliable alternative that does not require specialized equipment or skills. These assays can be performed using lateral flow devices, making them suitable for onsite detection or testing in the field. Isothermal amplification assays can also be combined with CRISPR/Cas technology to overcome non-specific amplification and cross-contamination issues. The collateral activity associated with some CRISPR/Cas systems has been successfully utilized for the visual detection of plant viruses.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Environmental Sciences
Qiqi Xue, Lingwei Sheng, Junjie Guo, Minghui Zou, Qiao Teng, Sijia Xu, Yuanyuan Li, Jinhong Zhao
Summary: In this study, a loop-mediated isothermal amplification (LAMP) method was successfully established for the detection of the Dermatophagoides farinae allergen. This method showed higher sensitivity, specificity, and efficiency compared to conventional PCR. It did not cross-react with other arthropod species commonly found in indoor dust. This study provides a potential model for the rapid detection of allergens produced by other house dust mites in the future.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Microbiology
Cassandra Suther, Sloane Stoufer, Yanjiao Zhou, Matthew D. Moore
Summary: Foodborne and enteric viruses impose a significant burden on public health and economy globally. Real time PCR, although considered a gold standard, has limitations in terms of portability, sample processing, and time to result. Isothermal amplification methods have shown promise as portable, sensitive, and rapid alternatives to PCR for detecting these viruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Materials Science, Multidisciplinary
Nazente Atceken, Muhammad Munzer Alseed, Sajjad Rahmani Dabbagh, Ali K. Yetisen, Savas Tasoglu
Summary: LAMP technology is a fast, efficient, and low-cost diagnostic method for pathogen detection, which can be applied to various bacteria, viruses, and parasitic pathogens. In addition, LAMP technology can also be used for SNP genotyping and mutation detection.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Multidisciplinary Sciences
Enoch Aninagyei, Adjoa Agyemang Boakye, Clement Okraku Tettey, Kofi Adjei Ntiri, Samuel Ohene Ofori, Comfort Dede Tetteh, Thelma Teley Aphour, Tanko Rufai
Summary: In this study, Plasmodium falciparum was detected in patients who tested negative using microscopy and mRDT, using the LAMP technique for 18s rRNA amplification. The study found that LAMP assay was more sensitive in detecting pre-clinical malaria compared to microscopy and mRDT. The prevalence of Plasmodium infection was higher in patients over 30 years old, males, rural dwellers, and patients seeking outpatient services. The study highlights the importance of using a sensitive technique like LAMP to increase detection rates of Plasmodium infection.
Article
Food Science & Technology
Alfonsina Fiore, Ida Treglia, Gianni Ciccaglioni, Marco Francesco Ortoffi, Antonietta Gattuso
Summary: Changing eating habits and rising demand of food have led to an increase in foodborne diseases, particularly in industrialized countries. Contaminated ready-to-eat food can transmit Listeria monocytogenes, a foodborne pathogen responsible for listeriosis. This study successfully applied a colorimetric Loop-mediated isothermal amplification (LAMP) assay to rapidly detect L. monocytogenes in experimentally contaminated RTE meat samples. The results showed high specificity and sensitivity compared to traditional methods, suggesting that colorimetric LAMP assays can be used as a screening tool for L. monocytogenes in ready-to-eat meat food.
Article
Virology
Olympia E. Anastasiou, Caroline Holtkamp, Miriam Schafer, Frieda Schon, Anna Maria Eis-Huebinger, Andi Krumbholz
Summary: This study examined the reliability of a commercial LAMP assay in directly detecting SARS-CoV-2 genomes in respiratory samples without nucleic acid extraction. The assay exhibited excellent specificity but moderate sensitivity, making it a promising tool for rapid on-site testing or verification of reactive rapid tests.
Article
Infectious Diseases
Banchamlak Tegegne, Sisay Getie, Wossenseged Lemma, Abu Naser Mohon, Dylan R. Pillai
Article
Infectious Diseases
Abebe A. Fola, Elma Nate, G. L. Abby Harrison, Celine Barnadas, Manuel W. Hetzel, Jonah Iga, Peter Siba, Ivo Mueller, Alyssa E. Barry
INFECTION GENETICS AND EVOLUTION
(2018)
Article
Infectious Diseases
Andreea Waltmann, Cristian Koepfli, Natacha Tessier, Stephan Karl, Abebe Fola, Andrew W. Darcy, Lyndes Wini, G. L. Abby Harrison, Celine Barnadas, Charlie Jennison, Harin Karunajeewa, Sarah Boyd, Maxine Whittaker, James Kazura, Melanie Bahlo, Ivo Mueller, Alyssa E. Barry
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Infectious Diseases
Lubaba Seid, William Stokes, Abebe Genetu Bayih, Sisay Getie, Aberham Abere, Habtie Tesfa, Dylan R. Pillai
BMC INFECTIOUS DISEASES
(2018)
Article
Infectious Diseases
Abebe A. Fola, Eline Kattenberg, Zahra Razook, Dulcie Lautu-Gumal, Stuart Lee, Somya Mehra, Melanie Bahlo, James Kazura, Leanne J. Robinson, Moses Laman, Ivo Mueller, Alyssa E. Barry
Article
Biochemistry & Molecular Biology
Johanna Helena Kattenberg, Zahra Razook, Raksmei Keo, Cristian Koepfli, Charlie Jennison, Dulcie Lautu-Gumal, Abebe A. Fola, Maria Ome-Kaius, Celine Barnadas, Peter Siba, Ingrid Felger, James Kazura, Ivo Mueller, Leanne J. Robinson, Alyssa E. Barry
Article
Multidisciplinary Sciences
Tahir Eyayu, Teklehaimanot Kiros, Lemma Workineh, Meslo Sema, Shewaneh Damtie, Wasihun Hailemichael, Eninur Dejen, Tegenaw Tiruneh
Summary: Intestinal parasitic infections are highly prevalent globally, especially in developing countries. This study conducted at Sanja Primary Hospital in Northwest Ethiopia found a high prevalence of intestinal parasitosis at 52.9%, with Entamoeba histolytica/dispar and Hookworm species being the leading causes. Factors such as illiteracy, frequent swimming, and lack of handwashing before meals were significantly associated with intestinal parasitic infections.
Article
Infectious Diseases
Mulenga C. Mwenda, Abebe A. Fola, Ilinca I. Ciubotariu, Conceptor Mulube, Brenda Mambwe, Rachael Kasaro, Moonga B. Hawela, Busiku Hamainza, John M. Miller, Giovanna Carpi, Daniel J. Bridges
Summary: Zambia has made significant progress in reducing malaria morbidity and mortality, with accurate diagnosis being crucial for achieving national elimination goals. A study compared the diagnostic performance of RDT and LM against PET-PCR, finding that LM had higher diagnostic accuracy than RDTs in Zambia, especially across a transmission gradient. However, both diagnostic methods showed reduced performance in low parasitaemia samples.
Article
Multidisciplinary Sciences
Tahir Eyayu, Melashu Yasin, Lemma Workineh, Tegenaw Tiruneh, Henok Andualem, Meslo Sema, Shewaneh Damtie, Aynework Abebaw, Birhanu Getie, Desalegn Andargie, Barnabas Achaw, Wubet Taklual
Summary: The study aimed to evaluate the diagnostic performance of the rK-39 strip test using urine for diagnosis of visceral leishmaniasis. The results showed that urine-based rK-39 strip test had high sensitivity, specificity, and strong agreement with serum-based rK-39 strip test results.
Article
Multidisciplinary Sciences
Tahir Eyayu, Gashaw Yimer, Lemma Workineh, Tegenaw Tiruneh, Meslo Sema, Biruk Legese, Andargachew Almaw, Yenealem Solomon, Birhanemaskal Malkamu, Ermias Sisay Chanie, Dejen Getaneh Feleke, Melkamu Senbeta Jimma, Seada Hassen, Aragaw Tesfaw
Summary: This study found that over one-third of school children were infected with at least one species of soil-transmitted helminths (STH); Ascaris lumbricoides was the most prevalent species; lack of shoe wearing habit and having untrimmed fingernails were identified as risk factors for STH infections.
Article
Immunology
Ilinca I. Ciubotariu, Jack Dorman, Nicole M. Perry, Lev Gorenstein, Jobin J. Kattoor, Abebe A. Fola, Amy Zine, G. Kenitra Hendrix, Rebecca P. Wilkes, Andrew Kitchen, Giovanna Carpi
Summary: By combining routine surveillance, genomic sequencing, and phylogeographic analysis, this study successfully tracked the spread and introduction events of SARS-CoV-2 variants in a university community. The analysis revealed the dominance of variants of concern, such as Alpha and Iota, during specific time periods, as well as the increasing frequency of the Gamma variant as campus surveillance was ending. The study also identified multiple independent introductions of the Gamma variant into the Purdue community, indicating both domestic and interstate spread.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Microbiology
Abebe A. Fola, Sindew M. Feleke, Hussein Mohammed, Bokretsion G. Brhane, Christopher M. Hennelly, Ashenafi Assefa, Rebecca M. Crudal, Emily Reichert, Jonathan J. Juliano, Jane Cunningham, Hassen Mamo, Hiwot Solomon, Geremew Tasew, Beyene Petros, Jonathan B. Parr, Jeffrey A. Bailey
Summary: Diagnosis and treatment of Plasmodium falciparum infections are crucial for malaria control and elimination. The study in Ethiopia revealed the presence of drug-resistant and diagnostic-resistant parasites, suggesting the need for close monitoring.
NATURE MICROBIOLOGY
(2023)
Article
Health Care Sciences & Services
Meslo Sema Berhanu, Seyfe Asrade Atnafie, Tahir Eyayu Ali, Aderaw Adamu Chekol, Habtamu Biazin Kebede
Summary: The study found a high prevalence of Schistosoma mansoni infection among primary school children in Northeast Ethiopia, with a good treatment response to praziquantel, supporting its continued use for treating infected school children.
ETHIOPIAN JOURNAL OF HEALTH SCIENCES
(2022)
Article
Pediatrics
Tahir Eyayu, Amilaku Wubie, Teklehaimanot Kiros, Tegenaw Tiruneh, Shewaneh Damtie, Meslo Sema, Aynework Abebaw, Ermias Sisay Chanie, Lemma Workineh
Summary: This study assessed the prevalence of intestinal parasitosis and associated factors among children aged 6 to 59 months in Northcentral Ethiopia. Protozoa parasites were found to be the most common cause of diseases in children, and factors such as irregular trimming of fingernails and eating unwashed fruits/vegetables were strongly associated with Intestinal Parasitic Infections (IPIs). Improving awareness among mothers/guardians about sources of infection and modes of transmission is important in reducing the prevalence of IPIs.
GLOBAL PEDIATRIC HEALTH
(2021)
Article
Public, Environmental & Occupational Health
Meseret Workineh, Biniam Mathewos, Beyene Moges, Adissu Gize, Sisay Getie, Olle Stendahl, Thomas Schon, Ebba Abate
ARCHIVES OF PUBLIC HEALTH
(2017)