期刊
IEEE SENSORS JOURNAL
卷 11, 期 11, 页码 2820-2824出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/JSEN.2011.2170969
关键词
Digital microfludic chip; mercury ion; mercury-specific oligonucleotide (MSO); water-soluble conjugated polymer
资金
- National Basic Research Program of China [2012CB933301, 2011CB309501]
- NSFC [61021064, 60936001]
- National Natural Science Foundation of China [60876037]
This paper reports a rapid and visual detection of mercury ion on a new developed digital microfluidic chip with electrostatic manipulated droplets, which employed a principle of conjugated polymers and label-free oligonucleotides for mercury ion detection. The complex of water-soluble conjugated polymer, poly (3-(3'-N,N,N-triethylamino-1'-pro-pyloxy)-4-methyl-2,5-thiophene hydrochloride) (PMNT) and a label-free, mercury-specific oligonucleotide (MSO) probe is used as a mercury detector. While the detector droplet is fixed on the microfluidic chip, the target mercury ionic droplet is transported to the detector and then mixed together by electrostatic force driving, resulting in the color of the detector changing from red to yellow. We demonstrate that the detection system is portable and highly selective, and it can identify micromolar Hg2+ concentrations within minutes with the naked eye.
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