期刊
HYPERTENSION
卷 61, 期 3, 页码 622-+出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/HYPERTENSIONAHA.111.199836
关键词
antihypertensive; nuclear factor-kappa B signaling; nucleus of the solitary tract; prorenin receptor
资金
- American Heart Association (AHA) [11SDG7420029]
- National Institutes of Health [HL33610]
- AHA [11SDG6770006]
The importance of the (pro) renin receptor (PRR) in the function of the central nervous system is increasingly evident because PRR seems to play a role in neuronal control of cardiovascular function. PRR expression is elevated in the nucleus of the solitary tract (NTS) of spontaneously hypertensive rats (SHR). In this study, we tested the hypothesis that altered activity of PRR in the NTS is linked to hypertension. Eight weeks of chronic knockdown of the NTS PRR, using recombinant adeno-associated virus type 2 (AAV2)-PRR-small hairpain RNA (shRNA)-mediated gene transduction, caused a significant increase in mean arterial pressure (MAP) in the SHR (shRNA, 173 +/- 5; Control, 151 +/- 6 mm Hg) but not in Wistar Kyoto rats (shRNA, 108 +/- 7; Control, 106 +/- 6 mm Hg). The MAP elevation in the SHR was associated with decreased inflammatory markers tumor necrosis factor-alpha, interleukin-6, C-C motif ligand 5, and their transcription factor, nuclear factor-kappa B. Consistent with the pressor effects of the PRR knockdown, acute bilateral NTS injection of human renin (2 pmol/side) decreased MAP and heart rate (HR) in SHR (Delta MAP, -38 +/- 4 mm Hg; Delta heart rate, -40 +/- 10 bpm), with negligible responses in Wistar Kyoto rats (Delta MAP, -4 +/- 3 mm Hg; Delta heart rate, -12 +/- 7 bpm). These effects in SHR were attenuated (80%) by prorenin handle region peptide but were not affected by angiotensin II type 1 or angiotensin II type 2 receptor blockers. Finally, PRR activation in SHR neuronal cultures by prorenin activated nuclear factor-kappa B and increased mRNA levels of interleukin-1 beta (250-fold), tumor necrosis factor-alpha (32-fold), interleukin-6 (35-fold), C-C motif ligand 5 (12-fold), and interleukin-10 (7-fold) in a nuclear factor-kappa B-dependent but angiotensin II type 1 receptor-independent manner. Therefore, NTS PRR mediates antihypertensive effects via an angiotensin II-independent mechanism in SHR, which involves stimulation of the nuclear factor-kappa B-cytokine signaling pathway. (Hypertension. 2013;61:622-627.) circle Online Data Supplement
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