期刊
HYPERTENSION
卷 54, 期 6, 页码 1262-U46出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/HYPERTENSIONAHA.109.139741
关键词
receptors; angiotensin II; stress; blood pressure; heart rate; immunohistochemistry; mice
资金
- National Health and Medical Research Council, Australia [472680, 472652]
We determined whether genetic deficiency of angiotensin II Type 1A (AT(1A)) receptors in mice results in altered neuronal responsiveness and reduced cardiovascular reactivity to stress. Telemetry devices were used to measure mean arterial pressure, heart rate, and activity. Before stress, lower resting mean arterial pressure was recorded in AT(1A)(-/-) (85 +/- 2 mm Hg) than in AT(1A)(+/+) (112 +/- 2 mm Hg) mice; heart rate was not different between groups. Cage-switch stress for 90 minutes elevated blood pressure by +24 +/- 2 mmHg in AT(1A)(+/+) and +17 +/- 2 mmHg in AT(1A)(-/-) mice (P < 0.01), and heart rate increased by +203 +/- 9 bpm in AT(1A)(+/+) and +121 +/- 9 bpm in AT(1A)(-/-) mice (P < 0.001). Locomotor activation was less in AT(1A)(-/-) (3.0 +/- 0.4 U) than in AT(1A)(+/+) animals (6.0 +/- 0.4 U), but differences in blood pressure and heart rate persisted during nonactive periods. In contrast to wild-type mice, spontaneous baroreflex sensitivity was not inhibited by stress in AT(1A)(-/-) mice. After cage-switch stress, c-Fos immunoreactivity was less in the paraventricular (P < 0.001) and dorsomedial (P = 0.001) nuclei of the hypothalamus and rostral ventrolateral medulla (P < 0.001) in AT(1A)(-/-) compared with AT(1A)(+/+) mice. Conversely, greater c-Fos immunoreactivity was observed in the medial nucleus of the amygdala, caudal ventrolateral medulla, and nucleus of the solitary tract (P < 0.001) of AT(1A)(-/-) compared with AT(1A)(-/-) mice. Greater activation of the amygdala suggests that AT(1A) receptors normally inhibit the degree of stress-induced anxiety, whereas the lesser activation of the hypothalamus and rostral ventrolateral medulla suggests that AT(1A) receptors play a key role in autonomic cardiovascular reactions to acute aversive stress, as well as for stress-induced inhibition of the baroreflex. (Hypertension. 2009; 54: 1262-1268.)
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