Article
Cardiac & Cardiovascular Systems
Alexander Akhmedov, Margot Crucet, Branko Simic, Simon Kraler, Nicole R. Bonetti, Caroline Ospelt, Oliver Distler, Adrian Ciurea, Luca Liberale, Matti Jauhiainen, Jari Metso, Melroy Miranda, Rose Cydecian, Lena Schwarz, Vera Fehr, Rita Zilinyi, Mohammad Amrollahi-Sharifabadi, Lydia Ntari, Niki Karagianni, Frank Ruschitzka, Reijo Laaksonen, Paul M. Vanhoutte, George Kollias, Giovanni G. Camici, Thomas F. Luescher
Summary: This study aimed to identify the molecular mechanism of endothelial dysfunction in a mouse model of rheumatoid arthritis (RA) and in patients with RA. The results showed that increased TNF alpha levels contribute to RA and endothelial dysfunction by increasing vascular oxLDL content and activation of the LOX-1/NF kappa B/Arg2 pathway, resulting in reduced NO bioavailability and decreased cGMP levels. Anti-TNF alpha treatment improved both articular symptoms and endothelial function by reducing LOX-1, vascular oxLDL, and Arg2 levels.
CARDIOVASCULAR RESEARCH
(2022)
Article
Urology & Nephrology
Jiafa Ren, Kang Liu, Buyun Wu, Xiaohan Lu, Lianqin Sun, Jamie R. Privratsky, Changying Xing, Matthew J. Robson, Huijuan Mao, Randy D. Blakely, Koki Abe, Tomokazu Souma, Steven D. Crowley
Summary: This study examines the expression of IL-1 and IL-1R1 in renal cells and infiltrating immune cells during AKI and demonstrates opposing effects of IL-1 receptor signaling on AKI. Disrupting IL-1R1 activation in tubules while preserving IL-1R1 activation in endothelial cells may provide better renal protection.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Cell Biology
Rusan Catar, Lei Chen, Hongfan Zhao, Dashan Wu, Julian Kamhieh-Milz, Christian Luecht, Daniel Zickler, Alexander W. Krug, Christian G. Ziegler, Henning Morawietz, Janusz Witowski
Summary: Atherosclerotic artery disease is a major global cause of death and healthcare burden. This study analyzes the effects of native LDL and oxidized LDL on the expression of their receptors in endothelial cells. The results show that native LDL mainly signals through LDLR receptors, while oxidized LDL mainly signals through LOX-1 receptors, and both engage different signaling pathways involving ERK1/2, p38, AP-1, and NF-kappa B transcription factors.
Article
Chemistry, Multidisciplinary
Tarik Bozoglu, Seungmin Lee, Tilman Ziegler, Victoria Jurisch, Sanne Maas, Andrea Baehr, Rabea Hinkel, Amelie Hoenig, Anjana Hariharan, Christina Inyeop Kim, Simon Decker, Haider Sami, Tobias Koppara, Ruppert Oellinger, Oliver J. Muller, Derk Frank, Remco Megens, Peter Nelson, Christian Weber, Angelika Schnieke, Markus Sperandio, Gianluca Santamaria, Roland Rad, Alessandra Moretti, Karl-Ludwig Laugwitz, Oliver Soehnlein, Manfred Ogris, Christian Kupatt
Summary: The study demonstrates a method of retargeting adeno-associated viruses (AAVs) to endothelial cells by coating them with second-generation polyamidoamine dendrimers (G2) linked to endothelial-affine peptides (CNN). This approach improves gene transfer efficiency, potentially enabling applications in vascular and atherosclerosis models.
Review
Biochemistry & Molecular Biology
Christelle Bergeron, Andre M. Cantin
Summary: Rare diseases affect 400 million individuals globally, and cystic fibrosis, a genetic disease, has seen advancements in potential therapies that aim to restore CFTR function, showing promise in improving the lives of patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Ji Xiao-hao, Wang Bao-liang, Wang Xiao-di, Wang Xiao-long, Liu Feng-zhi, Wang Hai-bo
Summary: This study compared the aroma compositions and concentrations of Kyoho and 87-1 grapes during berry development using SPME and GC-MS, revealing differences in esters and terpenes between the two varieties. Gene expression analysis suggested potential reasons for the differences, offering insights for improving grape aromatic quality.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Review
Cardiac & Cardiovascular Systems
Genmao Cao, Xuezhen Xuan, Ruijing Zhang, Jie Hu, Honglin Dong
Summary: Genetic factors have been increasingly recognized for their role in human diseases, with the advancement of gene-editing technology offering promise for refractory and genetic diseases. Despite obstacles like limited understanding of cardiovascular disease and ethical concerns, researchers are exploring the application of gene therapy in cardiovascular diseases and developing new delivery systems.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cell Biology
Celia Maria Quinonez-Flores, Salma Marcela Lopez-Loeza, Cesar Pacheco-Tena, Perla Maria Munoz-Morales, Samara Acosta-Jimenez, Susana Aidee Gonzalez-Chavez
Summary: The study found differences in gene stabilities between CIA and SpAD models in DBA/1 mice, with EF1a, RPL13A, and TBP identified as the best reference genes for different models. These genes enabled data normalization for quantifying inflammatory cytokines, indicating an increase in the expression of IL-1B, IL-12B, IL-17A, and IL-6 in the inflamed joints. Results highlight the importance of validating reference genes before each experiment for obtaining valid and reproducible qPCR data.
INFLAMMATION RESEARCH
(2021)
Article
Medicine, Research & Experimental
K. B. Timizheva, M. M. Azova, A. Ait Aissa, A. Aghajanyan, L. Tskhovrebova, M. L. Blagonravov
Summary: Restenosis remains a main complication after percutaneous coronary interventions in patients with coronary heart disease. The study focused on polymorphic loci of genes related to endothelin and endothelial NO synthase, showing different associations with restenosis development in older patients and timing of in-stent restenosis. These results could provide valuable insights for assessing restenosis risk factors and guiding treatment choices for patients with coronary heart disease prior to elective surgical intervention.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Oncology
Chao Liu, Ruiqi Liu, Bojun Wang, Jie Lian, Yang Yao, Haoxiu Sun, Chunhui Zhang, Lin Fang, Xin Guan, Jiaqi Shi, Shuling Han, Fei Zhan, Shengnan Luo, Yuanfei Yao, Tongsen Zheng, Yanqiao Zhang
Summary: IL-17A promotes resistance to anti-PD-1 therapy by increasing PD-L1 expression through the p65/NRF1/miR-15b-5p axis. Blocking IL-17A improves the efficacy of anti-PD-1 therapy in MSS CRC murine models, suggesting IL-17A as a potential therapeutic target to sensitize patients with MSS CRC to ICI therapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Biochemistry & Molecular Biology
Ana Alfano, Eduardo G. A. Cafferata, Mariela Gangemi, Alejandro Nicola Candia, Cristian M. Malnero, Ismael Bermudez, Mauricio Vargas Lopez, Gregorio David Rios, Cecilia Rotondaro, Nicasio Cuneo, David T. Curiel, Osvaldo L. Podhajcer, Maria Veronica Lopez
Summary: More than one million women worldwide are diagnosed with gynecological cancer each year. Most cases are diagnosed at a late stage due to a lack of symptoms or limited access to prevention in low-resource countries. The oncolytic adenovirus AR2011 has shown promising results in inhibiting the growth of ovarian, uterine, and cervical cancers in vitro, as well as synergizing with chemotherapy. In animal models, AR2011 has demonstrated efficacy in treating ovarian cancer and inducing an abscopal effect. It is a potential novel medicine for intraperitoneal disseminated ovarian cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Zhi Jie Li, Qian Qian Yang, You Lang Zhou
Summary: Tendon injuries can be chronic or acute, resulting in pain and loss of function. The natural healing process is complex and clinical outcomes vary based on tendon tissue position. Treatment options are diverse and while many therapies are not mature, with continuous optimization, the hope for effective treatments is not far away.
FRONTIERS IN MEDICINE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Hisashi Goto, Takeshi Kikuchi, Yuhei Takayanagi, Yosuke Kamiya, Yuki Suzuki, Shotaro Kawamura, Noritaka Sawada, Jun-ichiro Hayashi, Akio Mitani
Summary: This study aimed to investigate the role of Ebi3-related cytokines in experimental periodontitis using Ebi3 knockout mice. The results showed that Ebi3 KO mice exhibited more severe alveolar bone resorption and higher levels of IL-17A expression, while IL-10 expression was lower. Injection of recombinant IL-35 reduced ligature-induced alveolar bone resorption in Ebi3 KO mice. These findings suggest that Th17 cells exacerbate experimental periodontitis in mice lacking Ebi3, and IL-35 may play a critical role in inhibiting periodontal tissue destruction.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2023)
Article
Immunology
Liangliang Wu, Shengzhi Xie, Lingxiong Wang, Jinfeng Li, Lu Han, Boyu Qin, Guoqing Zhang, Qiyan Wu, Wenjuan Gao, Lijun Zhang, Huafeng Wei, Tianyi Liu, Shunchang Jiao
Summary: The ratio of IP-10 to IL-8 in peripheral blood can serve as a biomarker for predicting treatment response and survival in patients receiving immune-combined chemotherapy for lung cancer and multiple cancers. The combination of IL-8 and IP-10 as predictors shows improved specificity and sensitivity compared to using them alone, with potential broad clinical applications in treating intractable neoplasms.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
He-Liang Wu, Zhi-Ran Yang, Li-Jun Yan, Yan-Dong Su, Ru Ma, Yan Li
Summary: This review summarizes the gene biology features and protein structure of nucleoplasmin2 (NPM2) and explores its relationship with malignant peritoneal mesothelioma (MPM). The results show that NPM2 may play a key role in the development and progression of MPM. The expression of NPM2 is correlated with the pathology and survival rate of MPM patients.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yihong Feng, Masayuki Endo, Hiroshi Sugiyama
Summary: Nucleosomes, as the fundamental building blocks of chromatin, are highly dynamic and play vital roles in the formation of higher-order chromatin structures and gene regulation. Recent advances in epigenetics research have gradually unraveled nucleosome structures, histone modifications, nucleosome-binding proteins, and their functions. With the continuous development of research methods such as cryo-EM and next-generation sequencing, our understanding of nucleosomes is becoming broader and deeper.
Article
Chemistry, Multidisciplinary
Shubham Mishra, Soyoung Park, Tomoko Emura, Hidaka Kumi, Hiroshi Sugiyama, Masayuki Endo
Summary: Stimuli-responsive switching molecules such as arylazopyrazole (AAP) have shown promising potential in controlling photoinduced assembly and disassembly of DNA origami nanostructures. AAP can achieve repeated assembly and disassembly of DNA origami structures through alternating UV and visible-light irradiation, demonstrating its versatility as a photoswitching molecule.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Soumya Sethi, Hiroshi Sugiyama, Masayuki Endo
Summary: DNA nanotechnology is a promising toolkit for mimicking cell-cell and cell-matrix interactions, aiding in understanding and manipulating cellular functions. Future innovations in the field can be inspired by exploring the potentials of DNA-based nanostructures and addressing current challenges.
Article
Biochemistry & Molecular Biology
Yuki Hirose, Tomo Ohno, Sefan Asamitsu, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama
Summary: A cyclic pyrrole-imidazole polyamide (cPIP) was synthesized to bind to the abnormal expanded CAG/CTG repeat DNA sequences, showing high binding affinity and specificity for its target DNA sequence.
Article
Biochemistry & Molecular Biology
Ryu Tashiro, Ji Hye Yum, Soyoung Park, Hiroshi Sugiyama
Summary: The study showed that the photoreactivity of U-Br changes dramatically from hydrogen abstraction to cross-linking by changing the conformation of the duplex from the B-form to the A-form. Among three A-form structures, the largest amount of cross-linked products was observed when U-Br was incorporated into the RNA strand and the pyrene was conjugated to the 5 ' end of the DNA. These results indicate that the contact manner of pyrene was different between A- and B-form duplexes.
Article
Biochemistry & Molecular Biology
Kai Yu, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa
Summary: Naturally occurring ribozymes with modular architecture are promising for designing and assembling three-dimensional RNA nanostructures with catalytic abilities. In this study, RNA nanostructures with polygonal-shaped ribozyme oligomers were constructed using unit RNAs from the Tetrahymena group I intron, and ribozyme trimers with a triangular shape were dimerized by introducing pillar units. The resulting double-decker nanostructures containing six ribozyme units showed higher catalytic activity than the parent ribozyme trimers.
Article
Peripheral Vascular Disease
Tomoyasu Otsuki, Noboru Fukuda, Lan Chen, Takahiro Ueno, Masari Otsuki, Masanori Abe
Summary: This study demonstrates that upregulation of TWIST1 in GMCs from SHRs leads to increased expression of the C3 gene, potentially contributing to the synthetic phenotype of mesenchymal tissue in hypertension.
HYPERTENSION RESEARCH
(2022)
Article
Urology & Nephrology
Yasutaka Murata, Daisuke Obinata, Taro Matsumoto, Yuichiro Ikado, Koichiro Kano, Noboru Fukuda, Kenya Yamaguchi, Satoru Takahashi
Summary: This study successfully established a persistence SUI model and demonstrated the therapeutic effects of DFAT cell transplantation for persistence SUI. These findings suggest that DFAT cells may be a cell source for the treatment of SUI.
INTERNATIONAL UROLOGY AND NEPHROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sagun Jonchhe, Shankar Pandey, Christian Beneze, Tomoko Emura, Hiroshi Sugiyama, Masayuki Endo, Hanbin Mao
Summary: Researchers found that a DNA nanobowl significantly increased the stability of a human telomeric G-quadruplex and its binding with a ligand. By minimizing the proximity effect and attributing the increased affinity to the nanoconfinement effect, they provided insights into the mechanisms of binding events in nanocavities.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Vinodh J. Sahayasheela, Zutao Yu, Yuki Hirose, Ganesh N. Pandian, Toshikazu Bando, Hiroshi Sugiyama
Summary: Research suggests that targeting Gli-mediated transcription to inhibit the proliferation of cancer stem cells may be a promising strategy.
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2022)
Article
Biochemistry & Molecular Biology
Shingo Hirashima, Hiroshi Sugiyama, Soyoung Park
Summary: This study characterized 2FA-containing DNA molecules and found that they have similar structure and properties to native adenine due to their small size, but exhibit unique features due to their high electronegativity. This provides valuable information for future applications of 2FA-modified DNA.
Article
Biochemistry & Molecular Biology
Junnosuke Hatanaka, Yuki Hirose, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama
Summary: In this study, we synthesized linear pyrrole-imidazole polyamides (PIPs) that can selectively bind to DNA. These linear PIPs have lower molecular weights compared to commonly used hairpin PIPs. The binding affinity of the PIPs to DNA was improved by modifying the N-terminus of the linear PIPs. These findings provide insights for the design of effective linear PIPs.
Article
Cell & Tissue Engineering
Kei Utsunomiya, Takashi Maruyama, Satoshi Shimizu, Taro Matsumoto, Morito Endo, Hiroki Kobayashi, Koichiro Kano, Masanori Abe, Noboru Fukuda
Summary: Implantation of DFAT cells can improve ANCA glomerulonephritis through immunosuppressive effects, suggesting a potential cell therapy for this disease.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Tomoyasu Otsuki, Noboru Fukuda, Lan Chen, Akiko Tsunemi, Masanori Abe
Summary: In this study, we have demonstrated the role of complement 3 (C3) in inducing epithelial-mesenchymal transition (EMT) phenomenon and renal fibrosis in unilateral ureteral obstruction (UUO) kidney. We investigated the involvement of twist-related protein 1 (TWIST1) in these processes and found that TWIST1 upregulation leads to EMT and renal fibrosis through C3 upregulation. Our findings suggest that targeting TWIST1 with pyrrole-imidazole (PI) polyamides could be a potential therapy for renal fibrosis.
Article
Biochemistry & Molecular Biology
Takuya Hidaka, Kaori Hashiya, Toshikazu Bando, Ganesh N. Pandian, Hiroshi Sugiyama
Summary: This study constructed a class of compounds that can selectively alkylate mutant mitochondrial DNA, with the potential to treat mitochondrial diseases. These compounds have programmability and can be used to target pathogenic mutations associated with mitochondrial diseases in future studies.
CELL CHEMICAL BIOLOGY
(2022)
