Feeding ecology and basal food sources that sustain the Paradoxal frog Pseudis minuta: a multiple approach combining stomach content, prey availability, and stable isotopes

In the present study, we investigated ontogenetic diet shifts, feeding strategy, prey preferences, and basal food sources that sustain the Paradoxal frog (Pseudis minuta) based on stomach content, prey availability, and stable isotope (delta C-13, delta N-15) approaches. The feeding strategy analysis showed that the population can be considered a generalist species with each individual displaying a marked opportunism for different preys. Trophic positions estimated using nitrogen isotopic ratio (delta N-15) revealed that tadpoles are primary consumers, but post-metamorphic individuals shifted to secondary and tertiary trophic levels as they increase in body size. A stable isotopic mixing model revealed that most of the carbon (0.61-0.72) sustaining the post-metamorphic P. minuta is derived from the aquatic rather than the adjacent terrestrial environment. This finding suggests that the post-metamorphic P. minuta is strongly dependent on carbon sources that primarily originate in aquatic systems, regardless of the terrestrial or aquatic origins of the arthropods in its diet. Our results indicated that this species is a generalist-opportunistic predator that derives most of their carbon sources from the aquatic environment where it shows preference for aquatic preys with higher individual biomasses.