Article
Virology
Liyan Wang, Fan Yang, Yixin Xiao, Bin Chen, Fumin Liu, Linfang Cheng, Hangping Yao, Nanping Wu, Haibo Wu
Summary: This study obtained three monoclonal antibodies against influenza virus from mice immunized with the HA protein of A/Michigan/45/2015(H1N1), demonstrating strong preventive and therapeutic effects. Two amino acid mutations were discovered, and one of the antibodies still showed protective efficacy after 24 hours of infection.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Veterinary Sciences
Artur Summerfield, Heidi Gerber, Rebeka Schmitt, Matthias Liniger, Santina Grazioli, Emiliana Brocchi
Summary: This study demonstrates that monoclonal antibodies targeting FMDV O and A serotypes are able to opsonize the virus and induce cell death. Opsonization shows broader reactivity within the serotype and correlates better with vaccine dose compared to neutralization. Neutralization and opsonization titers are similarly predictive of protection.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Srinu Tumpara, Elena Korenbaum, Mark Kuehnel, Danny Jonigk, Beata Olejnicka, Michael Davids, Tobias Welte, Beatriz Martinez-Delgado, Sabina Janciauskiene
Summary: The study identified a novel antibody, C42, which specifically recognizes the C-36 peptide of AAT in various immunoassay formats. The antibody showed effectiveness in dot- and Western blotting, confocal laser microscopy, and flow cytometry assays, suggesting its potential as an important tool in AAT-related research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Chang Sun, Hongtao Zhu, Sarah Clark, Eric Gouaux
Summary: This study reveals the existence of different types of GABA receptors in the brain and the significant role of endogenous neurosteroids in modulating receptor function. These findings contribute to the development of subtype-specific drugs.
Article
Pharmacology & Pharmacy
Yuanjun Zeng, Feibo Song, Guoxing Luo, Han Yang, Cao Li, Wei Liu, Tingdong Li, Shiyin Zhang, Yingbin Wang, Chenghao Huang, Shengxiang Ge, Jun Zhang, Ningshao Xia
Summary: In this study, researchers generated 50 mAbs by immunizing mice with truncated VP4*. Some of these mAbs were able to neutralize RVs. These mAbs can be used as immunodiagnostic and research reagents, and may provide important tools for exploring the neutralizing mechanisms and quality control of VP4* protein-based vaccines.
ANTIVIRAL RESEARCH
(2022)
Article
Plant Sciences
Kaewta Rattanapisit, Tharita Kitisripanya, Atthaphon Konyanee, Worapol Sae-Foo, Apisit Burapapiruin, Waraporn Putalun, Seiichi Sakamoto, Waranyoo Phoolcharoen, Gorawit Yusakul
Summary: The plant expression platform is a new source for producing IgG targeting small low-molecular-weight molecules. The study demonstrated that plant-produced functional IgG maintained the binding activity and specificity of IgG derived from mammalian cells, showing potential as an efficient and cost-effective platform for antibody production in immunoassays targeting low-molecular-weight compounds.
PLANT CELL REPORTS
(2021)
Article
Pharmacology & Pharmacy
Fan Yang, Sijing Yan, Linwei Zhu, Frederick X. C. Wang, Fumin Liu, Linfang Cheng, Hangping Yao, Nanping Wu, Rufeng Lu, Haibo Wu
Summary: This study generated a panel of neutralizing antibodies against A(H1N1) pdm09 influenza virus. Eleven antibodies demonstrated therapeutic protection in a mouse model of A(H1N1) pdm09 infection, and a bispecific antibody combining the specificities of two antibodies also showed effectiveness in preventing and treating A(H1N1) pdm09 infection.
ANTIVIRAL RESEARCH
(2022)
Review
Neurosciences
Yu Sun, Zechun Peng, Xiaofei Wei, Nianhui Zhang, Martin Wallner, Istvan Mody, Carolyn R. Houser
Summary: The decreased expression of the delta subunit of the GABA(A) receptor has been found to be associated with altered modulation of tonic inhibition in dentate granule cells in animal models of epilepsy and other disorders. This study aimed to investigate if viral transfection of delta subunits could restore network excitability in a mouse model of epilepsy. The results showed that transfection of delta subunits increased delta subunit expression, downregulated other GABA(A) receptor subunits, and restored more normal network excitability.
PROGRESS IN NEUROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jun Wu, Miao Zhao, Yu-chen Jin, Min Li, Ke-xin Yu, Hai-bo Yu
Summary: This study investigated the antiseizure effect of Schisandrin B (SchB) in mouse models of seizure and explored its underlying mechanisms. The results showed that SchB exhibited therapeutic potential in various mouse models of seizure and acted as a positive allosteric modulator of GABA(A)Rs and GlyRs.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Veterinary Sciences
Kexin Zhu, Dong Yu, Jiahui An, Yufeng Li
Summary: In this study, a monoclonal antibody (mAb) was discovered that can be used to prevent and control G. parasuis infection in vivo and in vitro, suggesting that Fe (3+) ABC transporter substrate-binding protein is an immunodominant antigen and a promising candidate for subunit vaccine development.
VETERINARY RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Pardis Mokhtary, Zeinab Pourhashem, Akram Abouei Mehrizi, Claudia Sala, Rino Rappuoli
Summary: Monoclonal antibodies are a revolutionary class of medications that can bind specific targets and receptors to combat various diseases. New technologies, such as gene delivery, have been developed to improve their efficacy and delivery routes, enabling them to be used in the treatment of viral infections and outbreaks.
Article
Biochemistry & Molecular Biology
Jun Nishida, Shigeyuki Shichino, Tatsuya Tsukui, Mayumi Hoshino, Tomoko Okada, Kyoko Okada, Yuri Yi, Seiko Toraya-Brown, Miho Mochizuki, Ryouta Koizumi, Katrin Ishii-Schrade, Kaori Denda-Nagai, Tatsuro Irimura
Summary: In this study, two monoclonal antibodies (mAbs) that recognize different glycoforms of Muc21 were generated. mAb 1A4-1 recognized Muc21 carrying glycans terminated with galactose residues, while mAb 18A11 recognized Muc21 carrying sialylated glycans. These antibodies are useful for detecting the expression of Muc21 in normal epithelia and cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Guangwei Han, Haotian Yang, Huiling Xu, Suya Zheng, Yingxiang Li, Zehui Liu, Fang He
Summary: Novel antiviral peptides derived from pCD163 were developed, which showed inhibitory effects on PRRSV of different lineages and modulated the NF-kappa B pathway. These findings deepen the understanding of the interaction between PRRSV and pCD163 receptor, and provide alternative universal antiviral strategies against PRRSV.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Veterinary Sciences
Zhenyang Guo, Siyu Zhang, Hu Xu, Wansheng Li, Chao Li, Jing Zhao, Bangjun Gong, Qi Sun, Lirun Xiang, Hongyuan Zhao, Qian Wang, Guohui Zhou, Yandong Tang, Tongqing An, Xuehui Cai, Zhijun Tian, Hongliang Zhang, Jinmei Peng
Summary: In this study, a monoclonal antibody 1H5 against the gE glycoprotein was prepared using a novel immunization and screening strategy. The immunofluorescence assay results showed that this monoclonal antibody specifically recognized both classic and variant strains of pseudorabies virus. The linear epitopes of B cells recognized by the monoclonal antibody were further identified, and the monoclonal antibody 1H5 bound at the novel epitope (67)RRAG(70), which is conserved in almost all pseudorabies virus strains.
VETERINARY SCIENCES
(2023)
Article
Multidisciplinary Sciences
Craig A. Sexton, Reka Penzinger, Martin Mortensen, Damian P. Bright, Trevor G. Smart
Summary: This study reveals that spontaneous gating of GABA(A) receptors requires incorporation of beta 3 subunits, which is modulated by protein kinases and neurosteroids. This spontaneous activity alters neural network activity by regulating tonic inhibition.
NATURE COMMUNICATIONS
(2021)
Review
Neurosciences
Suchitra Joshi, Jaideep Kapur
Article
Clinical Neurology
Nadia Adotevi, Ewa Lewczuk, Huayu Sun, Suchitra Joshi, Natalia Dabrowska, Sarah Shan, John Williamson, Jaideep Kapur
ANNALS OF NEUROLOGY
(2020)
Article
Medicine, General & Internal
James M. Chamberlain, Jaideep Kapur, Shlomo Shinnar, Jordan Elm, Maija Holsti, Lynn Babcock, Alex Rogers, William Barsan, James Cloyd, Daniel Lowenstein, Thomas P. Bleck, Robin Conwit, Caitlyn Meinzer, Hannah Cock, Nathan B. Fountain, Ellen Underwood, Jason T. Connor, Robert Silbergleit
Article
Clinical Neurology
Suchitra Joshi, William H. Roden, Jaideep Kapur, Laura A. Jansen
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2020)
Article
Neurosciences
Denise K. Grosenbaugh, Suchitra Joshi, Mark P. Fitzgerald, Kevin S. Lee, Pravin K. Wagley, Alexander F. Koeppel, Stephen D. Turner, Michael J. McConnell, Howard P. Goodkin
NEUROBIOLOGY OF DISEASE
(2020)
Article
Clinical Neurology
Tanveer Singh, Suchitra Joshi, John M. Williamson, Jaideep Kapur
Article
Clinical Neurology
Justyna Straub, Agnieszka Gawda, Pranav Ravichandran, Bailey McGrew, Elsa Nylund, Julianna Kang, Cassidy Burke, Iuliia Vitko, Michael Scott, John Williamson, Suchitra Joshi, Jaideep Kapur, Edward Perez-Reyes
Article
Neurosciences
Jaideep Kapur, Suchitra Joshi
Summary: Progesterone acts on neurons by activating its receptor and converting to neurosteroids, enhancing the expression of AMPARs. Its metabolite allopregnanolone mediates anxiolytic and sedative effects through enhanced GABA(A)Rs activation, showing opposing roles in regulating seizures and catamenial epilepsy.
NEUROSCIENCE LETTERS
(2021)
Article
Multidisciplinary Sciences
Meng Zhuang, Suchitra Joshi, Huayu Sun, Tamal Batabyal, Cassandra L. Fraser, Jaideep Kapur
Summary: Oxygen plays a critical role in maintaining neuron structure and function. Boron nanoparticles offer a non-invasive method for oxygen sensing in neurological research, demonstrating real-time ratiometric oxygen sensing with micron-level spatial and millisecond-level temporal resolution. This tool could provide valuable insights into neuronal mechanisms and excitability research.
SCIENTIFIC REPORTS
(2021)
Article
Clinical Neurology
Shinnosuke Shiono, Huayu Sun, Tamal Batabyal, Aleksandra Labuz, John Williamson, Jaideep Kapur, Suchitra Joshi
Summary: Activation of PRs in the EC and hippocampus increases neuronal excitability and worsens seizures. These receptors may play a role in catamenial epilepsy.
Article
Neurosciences
Jennifer Burnsed, Weronika Matysik, Lu Yang, Huayu Sun, Suchitra Joshi, Jaideep Kapur
Summary: Postnatal maturation of the motor cortex is vital for developing motor learning capacity. During the first few weeks after birth, there are numerous neuronal and synaptic changes in the motor cortex, which vary by region and layer, likely due to different developmental functions and needs. Layer II/III of the motor cortex plays a crucial role in receiving and integrating inputs and generating attentional signals for motor learning and planning. By studying the changes in layer II/III pyramidal neurons of the mouse motor cortex from neonatal to young adult stages, we found several changes including increased dendritic branching, neuronal excitability, glutamatergic synapse number, and synaptic transmission. These changes are crucial for ongoing plasticity and motor learning capacity during development. Understanding these changes can help inform future studies on the impact of early-life injury and experiences on motor learning and development capacity.
Article
Multidisciplinary Sciences
Suchitra Joshi, Cedric L. Williams, Jaideep Kapur
Summary: Progesterone and its receptors play a role in mating and reproduction, and this study investigates their role in spatial declarative memory. The results show that PRs are expressed in brain regions related to spatial memory in male mice. By examining the performance of PRKO mice in various cognitive tasks, including object recognition and spatial tasks, the authors found that PR deletion impaired spatial memory but did not affect fear conditioning. These findings provide new insights into the role of PRs in facilitating spatial memory in males.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Chengsan Sun, Shuqiu Zheng, Justin S. A. Perry, Geoffrey T. Norris, Mei Cheng, Fanzhen Kong, Rolf Skyberg, Jianhua Cang, Alev Erisir, Jonathan Kipnis, David L. Hill
Summary: Microglia play a crucial role in the refinement of central gustatory circuits during normal postnatal development. This process can be disrupted by restricting the mother's dietary sodium intake in the early prenatal period, when microglia progenitors migrate from the yolk sac to the brain. The pruning of gustatory terminal fields in the nucleus of the solitary tract (NST) is driven by NaCl-elicited taste activity and involves the complement system. Offspring of mothers with a low NaCl diet fail to prune the gustatory terminal fields, despite normal peripheral taste activity. However, the ability to prune can be rescued by activating myeloid cells postnatally.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Review
Clinical Neurology
Suchitra Joshi, Howard P. Goodkin
JOURNAL OF CLINICAL NEUROPHYSIOLOGY
(2020)
