期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 62, 期 1, 页码 55-62出版社
WILEY
DOI: 10.1111/lam.12508
关键词
16S rRNA gene sequencing; Acetoanaerobium sp WJDL-Y2; GC-MS; growth kinetics; kraft lignin degradation; response surface methodology
资金
- Natural Science Foundation of Shaanxi Province in China [2015JQ2052]
- Scientific Research Program by Shaanxi Provincial Education Department [14JK1546]
- Shaanxi Science and Technology Activities Foundation for the Returned Visiting Scholars
An anaerobic kraft lignin (KL)-degrading bacterial strain was isolated from sludge of a pulp and paper mill. It was characterized as Acetoanaerobium sp. WJDL-Y2 by 16S rRNA gene sequencing. The maximum KL degradation capability of strain Y2 was determined to be 24.9% on a COD basis under an optimal condition with temperature of 31.5 degrees C, initial pH of 6.8 and KL to nitrogen (as NH4Cl) ratio of 6.5 by mass. Growth kinetic studies showed that the KL tolerance of strain Y2 was relatively high (K-i = 8120.45 mg l(-1)). Analysing KL degradation products by GC-MS revealed the formation of low-molecular-weight aromatic compounds (LMWACs), including benzenepropanoic acid, syringic acid and ferulic acid. This indicates that strain Y2 can oxidize lignin structure's p-hydroxyphenyl (H) units, guaiacyl (G) units and syringyl (S). In addition, the inoculated sample also contained low-molecular acid compounds, such as hexanoic acid, adipic acid and 2-hydroxybutyric acid, further validating strain Y2's ability to degrade KL.
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