期刊
HUMAN IMMUNOLOGY
卷 72, 期 1, 页码 74-79出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2010.10.003
关键词
Ficolin-2; FCN2 gene polymorphisms; Malaria; Real time PCR; Gabon
类别
资金
- European Commission (TRANCHI)
Human ficolin-2 (L-ficolin; FCN2) is a serum protein binding to sugar moieties of different human micropathogens forcing phagocytosis. Here, we investigate the clinical significance of FCN2 in African children with either mild or severe malaria (n = 130 and n = 108, respectively) from Gabon by analyzing three promoter SNPs (-986G > A, -602G > A, and -4A > G) and one single nucleotide polymorphisms (SNP) in exon 8 (+6424G > T) using quantitative TaqMan, real-time polymerase chain reaction (PCR). In addition, we measured the ficolin-2 plasma levels at two time points: on admission (t(0), acute disease) and 4 weeks after treatment (t(1), healthy phase). Comparison of ficolin-2 plasma levels shows that ficolin-2 concentration is highest during acute severe disease. In addition, we determined polymorphisms in the promoter and all coding regions of FCN2 in 40 Gabonese. Linkage disequilibrium data revealed polymorphic allelic combination patterns in the FCN2 promoter region; strong allelic combinations at -986 and -4, and -557 and -64 were found. No FCN2 promoter haplotypes were significantly distributed between mild and severe cases. (c) 2011 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据