Article
Gastroenterology & Hepatology
Sarah C. Sasson, Stephanie M. Slevin, Vincent T. F. Cheung, Isar Nassiri, Anna Olsson-Brown, Eve Fryer, Ricardo C. Ferreira, Dominik Trzupek, Tarun Gupta, Lulia Al-Hillawi, Mari-lenna Issaias, Alistair Easton, Leticia Campo, Michael E. B. FitzPatrick, Joss Adams, Meenali Chitnis, Andrew Protheroe, Mark Tuthill, Nicholas Coupe, Alison Simmons, Miranda Payne, Mark R. Middleton, Simon P. L. Travis, Benjamin P. Fairfax, Paul Klenerman, Oliver Brain
Summary: Our study demonstrates that CD8(+) tissue resident memory T (T-RM) cells are the main activated T cell subset in ICI colitis. The pattern of gastrointestinal immunopathology in ICI colitis is distinct from ulcerative colitis at both the immune and epithelial-signaling levels. Activation of CD8+ T-RM cells correlates with clinical and endoscopic severity of ICI colitis.
Review
Immunology
Tess Van Meerhaeghe, Antoine Neel, Sophie Brouard, Nicolas Degauque
Summary: The role of B cells in the regulation of CD8 T cell survival, proliferation, and memory formation has been a topic of debate. B cells can act as antigen-presenting cells for CD8 T cells due to their high expression of MHC class I molecules. In various conditions, such as viral infections, autoimmune diseases, cancer, and allograft rejection, B cells have been shown to modulate CD8 T cell function. However, B-cell depletion therapies can impair CD8 T cell responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Catherine Verhaeghe, Marja Talikka, Alain Sewer, Nicolas Sierro, Mehdi Auberson, Dariusz Peric, David Bornand, Remi Dulize, Emmanuel Guedj, Patrick Nef, Sebastien P. Tabruyn, Julia Hoeng, Manuel C. Peitsch, Giuseppe Lo Sasso
Summary: This study investigated the potential anti-inflammatory activities of tobacco alkaloids nicotine and anatabine in a fully humanized UC mouse model. The results showed that nicotine significantly reduced acute colitis symptoms and improved colitis-specific endpoints. Anatabine also demonstrated similar effectiveness, although weaker or not significant. Gene expression analysis provided insights into the possible mechanism by which nicotine attenuated DSS-induced colitis in humanized mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Yoshiyuki Takahashi, Tadanobu Nagaya, Yugo Iwaya, Takuma Okamura, Atsuhiro Hirayama, Mai Iwaya, Takeshi Uehara, Takeji Umemura
Summary: This study found that CD8(+) lymphocyte infiltration is significantly higher in immune-related adverse event (irAE) colitis compared to other forms of colitis. The ratio of CD8(+)/CD4(+) lymphocytes is also significantly higher in irAE colitis. These findings suggest that CD8(+) lymphocyte infiltration and CD8(+)/CD4(+) ratio may be simple and useful biomarkers to distinguish irAE colitis from other forms of colitis.
DIGESTIVE DISEASES AND SCIENCES
(2023)
Article
Microbiology
Petra Adamkova, Petra Hradicka, Sona Gancarcikova, Monika Kassayova, Lubos Ambro, Izabela Bertkova, Martin Maronek, Silvia Farkasova Iannaccone, Vlasta Demeckova
Summary: The deviation in gut microbial composition is involved in various pathologies, including inflammatory bowel disease. Faecal microbiota transplant (FMT) can reverse changes in the microbiome and restore homeostasis, making it a promising approach to treat IBD. This study investigated the effect of FMT on the remission of acute inflammatory response in a DSS-induced rat colitis model, demonstrating significant shifts in dominant microbiota species under inflammatory conditions and the restorative effect of FMT treatment on microbial composition.
Article
Biology
Severine Menoret, Laurent Tesson, Severine Remy, Victor Gourain, Celine Serazin, Claire Usal, Aude Guiffes, Vanessa Chenouard, Laure-Helene Ouisse, Malika Gantier, Jean-Marie Heslan, Cynthia Fourgeux, Jeremie Poschmann, Carole Guillonneau, Ignacio Anegon
Summary: In this study, a Foxp3-EGFP rat transgenic line was created to genetically tag CD4(+) and CD8(+) FOXP3(+) regulatory T cells. CD4(+)EGFP(+) Treg were found to be 5-10 times more frequent than CD8(+)EGFP(+) Treg. RNAseq analysis provided insights into the transcriptome of CD8(+) Treg, allowing for a better understanding of their phenotype and function.
Article
Medicine, Research & Experimental
Hao-ming Xu, Jing Xu, Mei-feng Yang, Yu-jie Liang, Quan-zhou Peng, Yuan Zhang, Cheng-mei Tian, Yu-qiang Nie, Li-sheng Wang, Jun Yao, De-feng Li
Summary: Ulcerative colitis (UC) is a heterogeneous disorder with complex pathogenesis. This study aimed to evaluate genome-wide DNA methylation changes associated with the pathogenesis of UC. The results confirmed that Zbtb7b was demethylated in UC tissues and its expression was up-regulated in both UC patients and a colitis model. Over-expression of Zbtb7b activated the maturation of CD4(+)T cells and repressed the differentiation of DP CD4(+)CD8(+)T cells, resulting in the production of inflammatory cytokines in colonic tissue.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Petra Pfenninger, Laura Yerly, Jun Abe
Summary: CRISPR/Cas9 technology has revolutionized genetic engineering of primary cells, including CD8(+) T cells. The optimized nucleofection-based CRISPR/Cas9 genetic engineering method preserves the in vivo immune responses of naive CD8(+) T cells, but slightly impairs the expansion and survival of in vitro-activated CD8(+) T cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Hengyuan Guo, Lu Zhang, Yi Wang, Xingxiang He
Summary: The study demonstrated that HuR promotes apoptosis of colonic epithelial cells in rats with UC by competitively binding HMGB1 with miR-29a-3p. Knockdown of HuR alleviates UC-induced colitis in rats and inhibits the apoptosis of colon tissues.
CELLULAR SIGNALLING
(2021)
Article
Integrative & Complementary Medicine
Li Xue-Ming, Yuan Ding-Yi, Liu Ya-Hui, Zhu Lei, Qin Hong-Kun, Yang Yu-Bing, Li Yan, Yan Fang, Wang Ya-Jing
Summary: Colorectal cancer (CRC) is the third most lethal cancer in the world, and its development is closely related to colon inflammatory responses. Panax notoginseng saponins (PNS) have been found to have anti-inflammatory, anti-oxidant, and anti-tumor effects. This study demonstrated that PNS has a protective effect against colitis-associated colorectal cancer (CAC), reducing the severity of colitis and preventing the occurrence of CAC. PNS relieved immunosuppression of Treg cells in the CAC microenvironment by inhibiting the expression of IDO1 mediated by STAT1. Rh1, a metabolite of PNS, showed the strongest inhibitory effect on IDO1 enzyme activity. These findings suggest that PNS has significant chemopreventive and immunomodulatory properties on CAC.
CHINESE JOURNAL OF NATURAL MEDICINES
(2022)
Review
Biochemistry & Molecular Biology
Carmen Schiweck, Sharmili Edwin Thanarajah, Mareike Aichholzer, Silke Matura, Andreas Reif, Elske Vrieze, Andreas Weigert, Alexander Visekruna
Summary: The gut microbiota produces a variety of enzymes that can synthesize different metabolites, including short-chain fatty acids (SCFAs). Recent research has revealed a central role for microbe-derived SCFAs in the interaction between gut microbiota and the host, directly affecting the metabolic activity and epigenetic status of host T lymphocytes. SCFAs play a crucial biological role in promoting the development of regulatory T cells and the production of the anti-inflammatory cytokine interleukin-10 (IL-10). These small molecules can also circulate and modulate immune cell function in distant tissues, showing therapeutic potential in autoimmune and inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Xuqiu Lei, Natalia Ketelut-Carneiro, Liraz Shmuel-Galia, Weili Xu, Ruth Wilson, Tim Vierbuchen, Yongzhi Chen, Andrea Reboldi, Joonsoo Kang, Karen L. Edelblum, Doyle Ward, Katherine A. Fitzgerald
Summary: The expression of HNF4A in intestinal epithelial cells (IECs) is essential for the proper development and composition of the intraepithelial lymphocyte (IEL) compartment, as it regulates the expression of immune signaling molecules and shapes the IEL compartment to maintain intestinal homeostasis.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Review
Immunology
Xiaoqing Chen, Xue Liu, Yichao Jiang, Ningshao Xia, Chao Liu, Wenxin Luo
Summary: Chronic hepatitis B virus (HBV) infection is a major public health challenge, with over 250 million people worldwide infected. Priming of naive HBV-specific CD8 T cells is hindered, and this is closely related to abnormalities in the complex immune microenvironment. In this article, we summarize recent progress in understanding the abnormal priming of HBV-specific CD8 T cells and discuss corresponding immunotherapies to facilitate their functional recovery. We also highlight the importance of balancing viral clearance and pathological liver injury induced by CD8 T-cell activation during drug development.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sukhbir Kaur, Duha Awad, Richard P. P. Finney, Thomas J. J. Meyer, Satya P. P. Singh, Margaret C. C. Cam, Baktiar O. O. Karim, Andrew C. C. Warner, David D. D. Roberts
Summary: Elevated expression of CD47 is associated with poor survival in some cancers, but improved survival in cutaneous melanomas. CD47-deficient T cells showed altered gene expression and alternative splicing, including upregulation of MYCN mRNA. Thrombospondin-1 inhibited the expression of MYCN isoforms, as well as the induction of T cell transcripts, resulting in improved overall survival in melanoma patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Ruhui Yang, Mingyue Yang, Zehua Wu, Bingjin Liu, Mingzhu Zheng, Linrong Lu, Songquan Wu
Summary: Tespa1 is a key molecule in T-cell development and has been found to play an important role in antitumour CD8+T cell immunity. It regulates the growth of cancer cells, the number of CD8+T cells, and the expression of proteins involved in the IL-2 signaling pathway. Its absence leads to weakened antitumour abilities and increased PD-1 expression in CD8+TILs.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Nicolas Thiault, Julie Darrigues, Veronique Adoue, Marine Gros, Benedicte Binet, Corine Perals, Bertrand Leobon, Nicolas Fazilleau, Olivier P. Joffre, Ellen A. Robey, Joost P. M. van Meerwijk, Paola Romagnoli
Article
Immunology
Yirajen Vuddamalay, Mehdi Attia, Rita Vicente, Celine Pomie, Genevieve Enault, Bertrand Leobon, Olivier Joffre, Paola Romagnoli, Joost P. M. van Meerwijk
Review
Geriatrics & Gerontology
Julie Darrigues, Joost P. M. van Meerwijk, Paola Romagnoli
Review
Cell Biology
Cecile Apert, Paola Romagnoli, Joost P. M. van Meerwijk
Review
Immunology
Yirajen Vuddamalay, Joost P. M. van Meerwijk
FRONTIERS IN IMMUNOLOGY
(2017)
Article
Hematology
Lise Pasquet, Jean-Yves Douet, Tim Sparwasser, Paola Romagnoli, Joost P. M. van Meerwijk
Article
Immunology
Julie Tellier, Andry Andrianjaka, Rita Vicente, Nicolas Thiault, Genevieve Enault, Henri-Jean Garchon, Joost P. M. van Meerwijk, Paola Romagnoli
EUROPEAN JOURNAL OF IMMUNOLOGY
(2013)
Article
Immunology
Paola Romagnoli, James Dooley, Genevieve Enault, Rita Vicente, Bernard Malissen, Adrian Liston, Joost P. M. van Meerwijk
JOURNAL OF IMMUNOLOGY
(2012)
Article
Multidisciplinary Sciences
Celine Pomie, Rita Vicente, Yirajen Vuddamalay, Brita Ardesjoe Lundgren, Mark van der Hoek, Genevieve Enault, Jeremy Kagan, Nicolas Fazilleau, Hamish S. Scott, Paola Romagnoli, Joost P. M. van Meerwijk
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2011)
Review
Immunology
Jeremy Santamaria, Julie Darrigues, Joost P. M. van Meerwijk, Paola Romagnoli
IMMUNOLOGY LETTERS
(2018)
Article
Immunology
Veronigue Adoue, Benedicte Binet, Agathe Malbec, Joanna Fourquet, Paola Romagnoli, Joost P. M. van Meerwijk, Sebastian Amigorena, Olivier P. Joffre
Article
Immunology
Julie Darrigues, Jeremy C. Santamaria, Ariel Galindo-Albarran, Ellen A. Robey, Olivier P. Joffre, Joost P. M. van Meerwijk, Paola Romagnoli
Summary: Research has shown that in the thymus of young NOD mice, an exceptionally large number of Treg develop, with the majority being recirculating cells and only a few being newly developing cells. Once activated in the periphery, these Treg cells quickly inhibit the development of new Treg, leading to a particularly large Treg niche in the thymus of adult NOD mice.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Endocrinology & Metabolism
Ariel Galindo-Albarran, Sarah Castan, Jeremy C. Santamaria, Olivier P. Joffre, Bart Haegeman, Paola Romagnoli, Joost P. M. van Meerwijk
Summary: The antigen specificity of Treg is crucial in their in vivo activity. Comparative analysis between NOD and C57BL/6 mice showed comparable diversity of TCRs, suggesting genetic defects affecting the TCR diversity expressed by newly developed Treg may not be involved in the etiology of type 1 diabetes in NOD mice.
Article
Immunology
Cecile Apert, Ariel O. Galindo-Albarran, Sarah Castan, Claire Detraves, Heloise Michaud, Nicola McJannett, Bart Haegeman, Simon Fillatreau, Bernard Malissen, Georg Hollander, Saulius Zuklys, Jeremy C. Santamaria, Olivier P. Joffre, Paola Romagnoli, Joost P. M. van Meerwijk
Summary: This study reevaluated the role of IL-2 and IL-15 in the development of regulatory T-lymphocytes (Treg) and found that IL-2 and IL-15 play important non-redundant quantitative and qualitative roles in intrathymic Treg development.
FRONTIERS IN IMMUNOLOGY
(2022)
Meeting Abstract
Immunology
Y. Vuddamalay, R. Vicente, P. Romagnoli, J. P. M. van Meerwijk