Article
Biotechnology & Applied Microbiology
Yi-Xuan Wang, Zhu-Li Peng, Zhi-Wen Sun, Yan-Jun Pan, Hong Ai, Zhi-Hui Mai
Summary: This study investigates the role of miR-20 in promoting osteogenic differentiation in BMSCs and accelerating bone formation in a rabbit maxillary sinus bone defect model. Overexpression of miR-20a increased the levels of osteogenic markers and enhanced calcified nodule deposition in BMSCs, while anti-miR-20a treatment had the opposite effect. In the rabbit model, miR-20a delivery significantly improved bone density, osteoblast numbers, and collagen type 1 secretion compared to Bio-Oss bone powder.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yu-Chen Lee, Song-Chang Lin, Guoyu Yu, Ming Zhu, Jian H. Song, Keith Rivera, Darryl Pappin, Christopher J. Logothetis, Theocharis Panaretakis, Guocan Wang, Li-Yuan Yu-Lee, Sue-Hwa Lin
Summary: The study reveals that tumor-induced endothelial cells transitioning into osteoblasts lead to bone formation, enhancing the metastatic potential of prostate cancer cells. Tenascin C (TNC) is identified as a major protein secreted during this process, promoting prostate cancer cell migration through various signaling pathways. Targeting TNC may hold promise for prostate cancer therapy.
Article
Cell & Tissue Engineering
Liang Chen, Ri-Yan Zhang, Jun Xie, Jia-Yi Yang, Kang-Hao Fang, Chen-Xuan Hong, Rong-Bo Yang, Najeeb Bsoul, Lei Yang
Summary: The study found that catalpol can enhance the osteogenic ability of bone marrow mesenchymal stem cells and promote BMSC-mediated angiogenesis by activating the JAK2/STAT3 signaling pathway. This suggests that catalpol may be an ideal therapeutic agent for the clinical treatment of osteoporotic bone fractures.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Christoph J. Burckhardt, John D. Minna, Gaudenz Danuser
Summary: The research demonstrates that SH3BP4 regulates integrin surface expression in a signaling-dependent manner, impacting cell-matrix interactions. Decreased Akt kinase activity leads to SH3BP4 mediating endocytosis of neuropilin-1 and alpha 5/beta 1-integrins, inhibiting cell spreading.
DEVELOPMENTAL CELL
(2021)
Article
Engineering, Biomedical
Dake Hao, Ruiwu Liu, Tomas Gonzalez Fernandez, Christopher Pivetti, Jordan Elizabeth Jackson, Edwin Samuel Kulubya, Hong-Jiang Jiang, Hai-Yang Ju, Wen-Liang Liu, Alyssa Panitch, Kit S. Lam, J. Kent Leach, Diana L. Farmer, Aijun Wang
Summary: This study developed a biomaterial that regulates endogenous cell adhesion to promote vascularized bone regeneration. The biomaterial improved the adhesion of mesenchymal stem cells, osteoblasts, endothelial progenitor cells, and endothelial cells, leading to enhanced bone formation and vascularization in animal models.
BIOACTIVE MATERIALS
(2023)
Article
Cell Biology
Sarah Hochmann, Kristy Ou, Rodolphe Poupardin, Michaela Mittermeir, Martin Textor, Salaheddine Ali, Martin Wolf, Agnes Ellinghaus, Dorit Jacobi, Juri A. J. Elmiger, Samantha Donsante, Mara Riminucci, Richard Schaefer, Uwe Kornak, Oliver Klein, Katharina Schallmoser, Katharina Schmidt-Bleek, Georg N. Duda, Julia K. Polansky, Sven Geissler, Dirk Strunk
Summary: Multipotent stromal cells are attractive sources for cell therapy and tissue engineering, but their application is limited due to functional diversity. In this study, we compared the regenerative potential of different stromal cell types in vitro and in vivo and found that only bone marrow stromal cells (BMSCs) could fully regenerate critical-size femoral defects. This ability was attributed to cell type-specific epigenetic landscapes controlling transcriptional stromal differentiation networks. BMSCs initiated defect healing and eventually contributed to tissue remodeling.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Yong Li, JunKai Zhang, LiFu Chen, HaiTao Li, Jian Wang
Summary: In this study, a RADA16-W9 peptide gel scaffold was designed and its effectiveness in bone defect repair was validated through cell experiments and a mouse model.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Xiaogang Wang, Luli Ji, Jing Wang, Changsheng Liu
Summary: Osteoclasts play a crucial role in bone homeostasis, and their dysregulation is associated with bone diseases. Current clinical strategies for managing osteoclasts often perturb bone metabolism. This study proposed a novel approach of regulating osteoclast differentiation through mechanical stimulation. The researchers created a hydrogel that mimicked the physiological bone microenvironment and found that matrix stiffness could direct osteoclast fate in vitro and in vivo. Selecting the optimum matrix stiffness could finely regulate osteoclast differentiation and potentially be used in bone tissue engineering strategies.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Cui Zhang, Shali Wu, Erman Chen, Luyang Yu, Jinfu Wang, Mengrui Wu
Summary: This study reveals that the lncRNA AC132217.4 plays a critical role in BMSC osteogenesis, promoting the development and function of osteoblasts. AC132217.4 interacts with IGF2 mRNA to regulate its expression and activate the AKT pathway, thereby controlling osteoblast maturation and function. Additionally, the splicing factors SC35 and HNRNPA1 are involved in the biogenesis of AC132217.4, while the transcription factor ALX1 regulates the expression of AC132217.7 to promote osteogenesis. In vivo over-expression of AC132217.4 significantly promotes bone healing in mice.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Elia Bari, Fulvio Tartara, Fabio Cofano, Giuseppe di Perna, Diego Garbossa, Sara Perteghella, Marzio Sorlini, Delia Mandracchia, Lorella Giovannelli, Paolo Gaetani, Maria Luisa Torre, Lorena Segale
Summary: The study found that the mesenchymal stem cell-secretome Lyosecretome has a significant improvement effect on the osteoinductive and osteoconductive properties of titanium cages, leading to increased mineralized matrix when used during culturing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Wei Jiang, Nobuo Takeshita, Toshihiro Maeda, Chisumi Sogi, Toshihito Oyanagi, Seiji Kimura, Michiko Yoshida, Kiyo Sasaki, Arata Ito, Teruko Takano-Yamamoto
Summary: The study found that CTGF promotes chemotaxis of preosteoblasts through the CTGF-integrin alpha 5-Ras axis, facilitating new bone formation.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yun Bai, Xiaoshan Gong, Rui Dong, Zhen Cao, Ce Dou, Chuan Liu, Jianmei Li, Fei Kang, Jingjin Dai, Chunrong Zhao, Zhansong Tian, Jiulin Tan, Qijie Dai, Shiwu Dong
Summary: Researchers have identified a long non-coding RNA called HCAR that plays a promoting role in endochondral bone repair. The study found that HCAR upregulates the expression of Mmp13 and Vegfa, which contributes to cartilage matrix remodeling and angiogenesis. The interaction between HCAR and miR-15b-5p was also revealed.
Article
Developmental Biology
Xiaofei Li, Callie McLain, Michael S. Samuel, Michael F. Olson, Glenn L. Radice
Summary: The switch of cardiomyocytes from a proliferative to differentiated state after birth results in the loss of regenerative potential of the mammalian heart. Nonmuscle myosin IIB-mediated actomyosin contractility plays a role in cardiomyocyte cytokinesis during embryonic heart development, and reduction of NM IIB levels after birth suggests a role for cellular tension in the regulation of cardiomyocyte cell cycle activity in the postnatal heart. Activation of ROCK2 kinase domain promotes cardiomyocyte proliferation by increasing integrin and fibronectin expression and promoting Yap nuclear translocation, and reduction of myocardial integrin rescues the proliferation phenotype. These findings uncover the function of nonmuscle myosin contractility in promoting cardiomyocyte proliferation in the postnatal heart.
Review
Biochemistry & Molecular Biology
Bo Li, Yigan Wang, Yi Fan, Takehito Ouchi, Zhihe Zha, Longjiang Li
Summary: This article summarizes the characteristics of the newly discovered cranial stem cell population SuSCs, including their location, self-renewal ability, multipotency, contribution to injury repair, as well as the signaling pathways and molecular mechanisms associated with the regulation of SuSCs.
Article
Nanoscience & Nanotechnology
Xiaotao Xing, Haisen Huang, Xin Gao, Jian Yang, Qi Tang, Xun Xu, Yutao Wu, Maojiao Li, Cheng Liang, Lin Tan, Li Liao, Weidong Tian
Summary: Elimination of senescent bone marrow mesenchymal stem cells (BMSCs) can promote bone regeneration in the elderly, providing a novel approach for treating bone defects in aged individuals.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Oncology
Antonin Marchais, Maria Eugenia Marques da Costa, Bastien Job, Rachid Abbas, Damien Drubay, Sophie Piperno-Neumann, Olivia Fromigue, Anne Gomez-Brouchet, Francoise Redini, Robin Droit, Cyril Lervat, Natacha Entze-Werle, Helene Pacquement, Catherine Devoldere, Didier Cupissol, Damien Bodet, Virginie Gandemer, Marc Berger, Perrine Marec-Berard, Marta Jimenez, Gilles Vassal, Birgit Geoerger, Laurence Brugieres, Nathalie Gaspar
Summary: This study suggests that the composition of the osteosarcoma microenvironment is a significant characteristic for identifying hard-to-treat tumors in patients at the time of diagnosis, and it also defines the biological pathways and potential therapeutic targets associated with these tumors.
Article
Polymer Science
Vuk V. Filipovic, Marija M. Radic, Jovana S. Vukovic, Marija Vukomanovic, Marina Rubert, Sandra Hofmann, Ralph Mueller, Simonida L. J. Tomic
Summary: New composite scaffolds combining synthetic and natural polymers with ceramic components were developed for biomedical applications. The incorporation of nanohydroxyapatite did not significantly affect osteogenic cell differentiation, but had synergistic effects on the mechanical behavior, structure, and interactions with biological species.
Article
Materials Science, Multidisciplinary
Gian Nutal Schaedli, Helena David, Anke M. de Leeuw, Franklin Carlson, Lara Tenisch, Pascal Muff, Marina Rubert, Ralph Mueller
Summary: The use of high-resolution micro-CT scans combined with histology and Raman spectroscopy allows for the evaluation of the osteogenic properties of nanocomposite scaffolds. By analyzing the mineralization and collagenous extracellular matrix formation, the optimal composition of the scaffolds can be determined for promoting bone formation.
FRONTIERS IN MATERIALS
(2022)
Article
Engineering, Biomedical
Jianhua Zhang, Julia Griesbach, Marsel Ganeyev, Anna-Katharina Zehnder, Peng Zeng, Gian Nutal Schaedli, Anke de Leeuw, Yuxiao Lai, Marina Rubert, Ralph Mueller
Summary: The study reveals that mechanical stimulation can promote the formation of 3D bioprinted bone organoids, and long-term mechanical loading is necessary for increasing the density and stiffness of the organoids, as well as promoting osteoblast differentiation.
Review
Endocrinology & Metabolism
Lorenz C. Hofbauer, Bjoern Busse, Richard Eastell, Serge Ferrari, Morten Frost, Ralph Mueller, Andrea M. Burden, Fernando Rivadeneira, Nicola Napoli, Martina Rauner
Summary: Increased fracture risk is a serious complication of diabetes, leading to prolonged immobility and hospitalizations that can result in significant morbidity and mortality. In type 1 diabetes, bone mass and strength are reduced, leading to a five-fold increased risk of lifelong fractures. In type 2 diabetes, fracture risk is increased despite normal bone mass. The fragility of bones in diabetes can be attributed to cellular abnormalities, matrix interactions, immune and vascular changes, and musculoskeletal maladaptation to chronic hyperglycemia.
LANCET DIABETES & ENDOCRINOLOGY
(2022)
Article
Engineering, Biomedical
Jenny Gehlen, Wanwan Qiu, Gian Nutal Schadli, Ralph Mueller, Xiao-Hua Qin
Summary: Tomographic volumetric bioprinting (VBP) is a powerful tool for rapid solidification of cell-laden hydrogel constructs. This study explores the application of VBP in bone tissue engineering by merging GelMA bioresin with 3D endothelial co-culture. The optimal formulation for VBP of complex perfusable constructs with high cell viability was identified.
ACTA BIOMATERIALIA
(2023)
Article
Endocrinology & Metabolism
Kenneth Guangpu Yang, Elliott Goff, Ka-lo Cheng, Gisela A. Kuhn, Yujia Wang, Jack Chun-yiu Cheng, Yong Qiu, Ralph Muller, Wayne Yuk-wai Lee
Summary: The study aimed to validate abnormal osteocyte lacunar morphology in adolescent idiopathic scoliosis (AIS) through a large-scale comparison of morphological features between AIS patients and controls in spine and ilium. Results showed that AIS patients had more oblate osteocyte lacunae in the iliac bone, which is less affected by asymmetric loading of the deformed spine, and the shape of osteocyte lacunae in the iliac bone was associated with the radiological Cobb angle in AIS patients. Osteocyte lacunae on the concave side of scoliotic curves were more stretched in both AIS and congenital scoliosis (CS) groups, likely due to asymmetric mechanical loading.
Article
Multidisciplinary Sciences
Caitlyn J. Collins, Penny R. Atkins, Nicholas Ohs, Michael Blauth, Kurt Lippuner, Ralph Mueller
Summary: High resolution peripheral quantitative computed tomography (HR-pQCT) combined with longitudinal imaging protocol and finite element analysis can aid in the early diagnosis of bone disease, and assess bone formation, resorption, and the relationship with mechanical loading. This study found that HR-pQCT can detect subtle changes in bone quantity and quality that traditional measures cannot capture.
SCIENTIFIC REPORTS
(2022)
Review
Endocrinology & Metabolism
Matthias Walle, Danielle E. Whittier, Morten Frost, Ralph Mueller, Caitlyn J. Collins
Summary: Diabetes mellitus is associated with changes in bone microarchitecture, with T1DM primarily affecting trabecular bone and T2DM mainly impacting cortical bone. Adverse effects are more pronounced at the radius, suggesting mechanical loading may mitigate these changes. Identifying bone structure abnormalities in T1DM and T2DM could aid in developing animal models for skeletal fragility in diabetes.
CURRENT OSTEOPOROSIS REPORTS
(2022)
Article
Endocrinology & Metabolism
Matthias Walle, Dominic Eggemann, Penny R. Atkins, Jack J. Kendall, Kerstin Stock, Ralph Muller, Caitlyn J. Collins
Summary: Motion artefacts in high-resolution peripheral quantitative computed tomography (HR-pQCT) can degrade image quality and affect measurements of bone density, morphology, and mechanical properties. This study developed a Convolutional Neural Network (CNN) that can predict motion scores from HR-pQCT images and identify uncertain predictions that require manual confirmation. The CNN achieved high accuracy and substantial agreement, and can significantly reduce the time for quality assessment and control of HR-pQCT scans.
Article
Chemistry, Multidisciplinary
Wanwan Qiu, Jenny Gehlen, Margherita Bernero, Christian Gehre, Gian Nutal Schaedli, Ralph Mueller, Xiao-Hua Qin
Summary: Tomographic volumetric bioprinting (VBP) allows fast fabrication of cell-laden hydrogel constructs using a dynamic resin based on thiol-ene photo-clickable polyvinyl alcohol (PVA) and thermo-sensitive sacrificial gelatin. This technique enables rapid cell spreading, 3D osteogenic differentiation, and matrix mineralization. It also allows site-specific immobilization of molecules-of-interest inside a PVA hydrogel. Overall, this study introduces a synthetic dynamic photoresin that enables fast fabrication of functional ultrasoft hydrogel constructs with well-defined physicochemical properties.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Correction
Engineering, Biomedical
Jianhua Zhang, Esther Wehrle, Jolanda R. Vetsch, Graeme R. Paul, Marina Rubert, Ralph Mueller
BIOMEDICAL MATERIALS
(2023)
Article
Endocrinology & Metabolism
Sebastian Zanner, Elliott Goff, Samuel Ghatan, Eva Maria Woelfel, Charlotte Ejersted, Gisela Kuhn, Ralph Mueller, Morten Frost
Summary: Clinical studies have shown that microvascular disease (MVD) can affect bone microstructure and decrease bone strength in patients with type 2 diabetes mellitus (T2D). This study collected bone biopsies from T2D patients and found that MVD may alter the morphometric parameters of osteocyte lacunae. Additionally, the study revealed that trabecular bone lacunar density was associated with glycated hemoglobin (HbA1c), independent of MVD.
Meeting Abstract
Medicine, General & Internal
Valerio Luca Mainardi, Rubert Marina, Claudia Sabato, Anke De Leeuw, Chiara Arrigoni, Gabriele Dubini, Christian Candrian, Ralph Mueller, Matteo Moretti
SWISS MEDICAL WEEKLY
(2022)
Article
Materials Science, Biomaterials
Marija Vukomanovic, Lea Gazvoda, Nemanja Anicic, Marina Rubert, Danilo Suvorov, Ralph Muller, Sandra Hofmann
Summary: This study presents a novel multi-doped apatite material containing Mg2+, Sr2+, Zn2+ and Ga3+ ions, which shows good biocompatibility and antimicrobial activity while promoting bone regeneration.
BIOMATERIALS ADVANCES
(2022)