Article
Biochemistry & Molecular Biology
Thalita J. J. Bondancia, Camila Florencio, Graziela S. S. Baccarin, Cristiane S. S. Farinas
Summary: Cellulose nanostructures obtained from lignocellulosic biomass by the enzymatic route can offer advantages in terms of material properties and processing sustainability. The presence of auxiliary enzymes can promote the production of nanocellulose with improved properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Manuel Cuevas, Juan F. Garcia Martin, Vicente Bravo, Sebastian Sanchez
Summary: This study investigated the potential of producing bioethanol from olive stones through liquid hot-water pre-treatment and subsequent enzymatic hydrolysis. The results showed that under the most suitable conditions, 6.4 dm(3) of ethanol per 100 kg of olive stones could be produced through this process.
FERMENTATION-BASEL
(2021)
Article
Engineering, Environmental
Huda Al-Battashi, Nallusamy Sivakumar
Summary: This study successfully produced bioplastic PHB using waste paper as a substrate through batch and fed-batch simultaneous saccharification and fermentation (SSF) approach. The results showed that higher PHB yield was achieved with H2O2 pretreatment, while better hydrolysis was observed with H2O2 and Triton X-100 pretreatment. Furthermore, the fed-batch experiment resulted in a higher PHB yield within a shorter time compared to the batch experiment.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Marttin Paulraj Gundupalli, Santi Chuetor, Kraipat Cheenkachorn, Kittipong Rattanaporn, Pau-Loke Show, Yu-Shen Cheng, Malinee Sriariyanun
Summary: This study investigated the interference of wax in different lignocellulosic biomasses and found that dewaxing can improve the composition and functional groups of biomass, promote dissociation of lignocellulose fibrils, and increase the extraction of reducing sugars and ethanol yield. The wax obtained from the biomasses mainly consisted of FAME, alcohol, and alkane.
BIOENGINEERING-BASEL
(2021)
Article
Energy & Fuels
Rajnish Kumar, Om Prakash
Summary: Researchers optimized the fermentation conditions for bioethanol production from wheat-stalk straw in different seasons with minimal laboratory resources. It was found that the maximum bioethanol yield varied in different seasons, with the highest yield in the monsoon season. The enhancement in maximum bioethanol yield in the monsoon season was 8.42% and 12.57% compared to the summer and spring seasons, respectively.
Article
Energy & Fuels
Jyosthna K. Goli, Bee Hameeda
Summary: The study successfully utilized Typha latifolia as substrate for xylitol and bioethanol production through chemical pretreatment and enzymatic hydrolysis.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Engineering, Environmental
Mariana S. T. Amandio, Jorge M. S. Rocha, Ana M. R. B. Xavier
Summary: The European Green Deal is a set of policy initiatives aimed at achieving climate neutrality by 2050 through a green transition. In the pulp and paper industry, upgrading residues into value-added products within the forest-based circular economy model has been a focus. This study explores the possibility of using bark, a high volume residue in factories, for cellulosic ethanol production instead of burning it for energy generation. By implementing simultaneous saccharification and fermentation (SSF) in the bioethanol production process, the study found that longer pre-saccharification stage resulted in higher productivity and shifting from a batch to a fed-batch configuration further improved the overall productivity and bioethanol concentration.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Review
Agricultural Engineering
Tirath Raj, K. Chandrasekhar, A. Naresh Kumar, J. Rajesh Banu, Jeong-Jun Yoon, Shashi Kant Bhatia, Yung-Hun Yang, Sunita Varjani, Sang-Hyoun Kim
Summary: Cellulosic ethanol production is facing major sustainability issues due to changes in feedstock composition, natural resistance, and lack of cost-effective pretreatment and downstream processing. To address these challenges, a global overview of current cellulosic ethanol facilities, main roadblocks, and technical challenges has been outlined, along with advancements in R&D and solutions for low-cost sustainable fuel ethanol production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Siriwan Khanpanuek, Siriporn Lunprom, Alissara Reungsang, Apilak Salakkam
Summary: This study demonstrated the feasibility of using immobilized enzymes and cells on bacterial cellulose for ethanol production from cassava pulp through repeated-batch simultaneous saccharification and fermentation process. Optimization of enzyme ratio and CP concentration allowed for repeated use of immobilized enzymes and cells, resulting in high ethanol yield and production rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Agricultural Engineering
Afrasiab Khan Tareen, Imrana Niaz Sultan, Kiettipong Songprom, Nikhom Laemsak, Sarote Sirisansaneeyakul, Wirat Vanichsriratana, Pramuk Parakulsuksatid
Summary: The study investigated the production of ethanol from oil palm trunk chips using steam explosion, alkaline extraction, and fermentation. The optimized two-step pretreatment process showed efficient ethanol production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biology
Ahmad Faizal, Anca Awal Sembada, Neil Priharto
Summary: Duckweeds are considered as promising sources for bioethanol production due to their high starch content and fast growth rate. After optimizing the saccharification and fermentation process, four species of duckweeds showed potential in producing significant ethanol concentrations, indicating their suitability for bioethanol production.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Federico Battista, Caterina Padovan, Elisa Salvetti, Veronica Gatto, Fabio Rizzioli, Davide Bertasini, David Bolzonella
Summary: Cigarette butts, composed of cellulose acetate and tobacco residues, are a major waste in urban areas. This study aimed to utilize cigarette butts for the production of fermentable sugars and bioethanol. Through optimizing deacetylation of cellulose acetate and adding cellulase, the deacetylated cellulose was converted into fermentable sugars. These sugars were then used as substrates for bioethanol production by different yeasts.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2023)
Article
Biotechnology & Applied Microbiology
Iolanda Corrado, Nicoletta Cascelli, Georgia Ntasi, Leila Birolo, Giovanni Sannia, Cinzia Pezzella
Summary: In this study, a new microbial source of inulinase, Penicillium lanosocoeruleum, was identified, and the production of inulinase using inulin as a carbon source was optimized. The efficient hydrolysis of inulin in optimized conditions was demonstrated, and the integration of PlaI into the PHA production process showed promising results in SSF.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Forough Momayez, Mattias Hedenstrom, Stefan Stagge, Leif J. Jonsson, Carlos Martin
Summary: In this study, hydrolysis lignin was extracted using the green solvent gamma-valerolactone (GVL), and it was found that mixtures containing sulfuric acid resulted in higher solubilization and regeneration rates. Characterization of the regenerated lignins revealed that treatment temperature had a significant impact on molecular weight, with SA-GVL lignins exhibiting higher and broader distribution of molecular weight.
BIORESOURCE TECHNOLOGY
(2022)
Article
Energy & Fuels
Davood Mazaheri, Yasin Orooji, Melika Mazaheri, Mojtaba Saei Moghaddam, Hassan Karimi-Maleh
Summary: This study focused on optimizing the simultaneous saccharification and fermentation (SSF) process using pomegranate peel as a substrate to produce bioethanol. After hydrothermal pretreatment and separation of pectin and phenolic compounds, the SSF process with cellulase and Saccharomyces cerevisiae was performed and optimized. The optimal process conditions resulted in high ethanol production and efficiency, with 48.5% yield and 95.09% of the theoretical yield achieved.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Chemistry, Physical
Frederic Perz, Sebastian Bormann, Roland Ulber, Miguel Alcalde, Paul Bubenheim, Frank Hollmann, Dirk Holtmann, Andreas Liese
Article
Environmental Sciences
Charlotte Capitain, Sebastian Wagner, Joana Hummel, Nils Tippkoetter
Summary: Developing strong biogenic adhesives based on mussel-inspired catechol-containing polymers, the functionalization of chitosan with protocatechuic acid was studied. Through kinetic models, it was found that the reaction between primary amines and catechols is promoted by a low pKa value and neutral or mildly acidic pH. Spontaneous reactions between chitosan and PCA were confirmed at pH 5 and 6.5, leading to the development of a novel bio-adhesive with an adhesion strength of 4.56 MPa on sand blasted aluminum surfaces.
WASTE AND BIOMASS VALORIZATION
(2021)
Article
Biotechnology & Applied Microbiology
Dorina Strieth, Sarah Di Nonno, Judith Stiefelmaier, Jonas Kollmen, Doris Geib, Roland Ulber
Summary: Diazotrophic cyanobacteria can serve as living nitrogen supplier for plants, leading to enhanced growth rates when co-cultivated with plants and successful induction of artificial symbiosis.
ENGINEERING IN LIFE SCIENCES
(2021)
Article
Chemistry, Applied
Amr Abdelwareth, Ahmed Zayed, Mohamed A. Farag
Summary: The study analyzed the aroma composition associated with coffee seeds brewing, revealing distinct aroma profiles between different brewing methods. It also highlighted the impact of brewing and roasting on the aroma composition of Middle Eastern coffee blends.
Article
Biotechnology & Applied Microbiology
Alexander Akermann, Jens Weiermueller, Selina Lenz, Jens Christmann, Roland Ulber
Summary: A kinetic model was developed to predict lactate formation and cell growth of Lactobacillus delbrueckii subsp. lactis using different Brewers' spent grain liquors, showing the importance of carbohydrates to amino acids ratio in the fermentation medium. Both experiment and simulation confirmed the essential role of serine and alanine in the metabolism of the organism, with small deviations between the two in relation to media composition differences.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Winda Soerjawinata, Konstantin Schlegel, Natalie Fuchs, Anja Schueffler, Tanja Schirmeister, Roland Ulber, Percy Kampeis
Summary: This study compared the fermentation of filamentous fungus in a single-use bioreactor and a glass bioreactor, demonstrating their similarities in fungal metabolic activity and reproducibility under selected cultivation conditions.
ENGINEERING IN LIFE SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Dorina Strieth, Anna Schwarz, Judith Stiefelmaier, Niklas Erdmann, Kai Muffler, Roland Ulber
Summary: Phototrophic biofilms produce a complex matrix of extracellular polymeric substances (EPS) consisting mainly of polysaccharides, proteins, and lipids. Under different cultivation conditions, mixotrophic cultivation results in higher amounts of carbohydrates, lipids, and proteins in EPS compared to heterotrophic cultivation. Additionally, there is an increase in protein content and a decrease in carbohydrates and lipids content in EPS over the cultivation period.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Jens Weiermuller, Alexander Akermann, Wolfgang Laudensack, Jonas Chodorski, Lars M. Blank, Roland Ulber
Summary: This study presents three process routes for producing itaconate from BSG hydrolysates using engineered smut fungi as biocatalyst. The fermentation medium based on BSG hydrolysates achieved good yield and productivity. However, the addition of detoxified hydrothermal supernatant did not improve performance parameters but instead resulted in decreased yield and productivity.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lars Wommer, Patrick Meiers, Isabelle Kockler, Roland Ulber, Percy Kampeis
Summary: The use of magnetic beads in the purification of mRNA for vaccines is becoming increasingly important. The mRNA binds specifically to oligo(dT)-functionalized magnetic beads and can be separated using magnetic separation. This method allows for rapid purification of large amounts of mRNA to meet the demands of clinical trials.
ENGINEERING IN LIFE SCIENCES
(2021)
Article
Chemistry, Medicinal
Nesrine M. Hegazi, Hamada H. Saad, Mona M. Marzouk, Mohamed F. Abdel Rahman, Mahitab H. El Bishbishy, Ahmed Zayed, Roland Ulber, Shahira M. Ezzat
Summary: The study explores the metabolic profiles and chemosystematics relationship of two seagrass taxa in the Red Sea through UPLC-HRMS/MS analysis. It identifies 144 metabolites, including new phenolic acids, flavonoids, and steviol glycoside, and suggests categorizing Halophila, Thalassia, and Enhalus in three different taxonomic ranks based on their interspecific relationships. The evaluation of their antidiabetic potential indicates higher activity of Halophila in alleviating oxidative stress and dyslipidemia associated with diabetes.
Article
Chemistry, Applied
Ahmed Zayed, Marwa T. Badawy, Mohamed A. Farag
Summary: Citrus seeds contain a variety of bioactive compounds, including fatty acids, phytosterols, and tocopherols, as well as limonoids, dietary fibers, and flavonoids. These chemicals not only have nutritional value, but also have promising applications in areas such as biodiesel production, food enhancers, and antioxidants.
Article
Biotechnology & Applied Microbiology
A. Mehring, N. Erdmann, J. Walther, J. Stiefelmaier, D. Strieth, R. Ulber
Summary: The use of resazurin dye as an indicator for assessing viability and vitality across different species has shown promising results. The study found that increasing biomass positively correlates with reaction rate, providing insights into cell vitality. Furthermore, the experiment on stored suspension cultures revealed a decrease in both viability and urease activity over time.
JOURNAL OF BIOTECHNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Mohamed A. Salem, Ahmed Zayed, Magy E. Beshay, Mirna M. Abdel Mesih, Reem F. Ben Khayal, Fady A. George, Shahira M. Ezzat
Summary: Hibiscus sabdariffa L. (HS) calyx extracts have been widely used for the treatment of hypertension, liver dysfunctions, and diabetes, mainly attributed to the red pigments anthocyanins. In addition, HS has other food and industrial applications, including the preparation of metallic nanoparticles. Optimization of extraction processes can maximize the benefits of bioactive constituents from HS.
ADVANCES IN TRADITIONAL MEDICINE
(2022)
Review
Chemistry, Analytical
Sarah Di Nonno, Roland Ulber
Summary: In recent decades, there has been a growing trend towards the use of smartphone-based analysis systems, mainly due to the ubiquity of smartphones, their increasing computing capacity, their relatively low cost, and the ability to acquire and process data simultaneously. Smartphone integrated sensors, such as CMOS sensors, enable optical analysis methods like colorimeters, photometers, and spectrometers. This review explores the state-of-the-art smartphone-based optical analysis systems in various applications, organized into three sections focusing on colorimeters, photo- and spectrometers, and fluorimeters.
Article
Biotechnology & Applied Microbiology
Jonas Chodorski, Jan Hauth, Dorina Strieth, Andreas Wirsen, Roland Ulber
Summary: Despite being an important topic in biofilm research, we still know little about diffusion in biofilms. The study shows that external factors can affect the diffusion behavior and growth rate of biofilms, while also revealing differences in diffusion inside biofilms depending on their position in the flow-cell.
ENGINEERING IN LIFE SCIENCES
(2021)