Article
Chemistry, Analytical
Qiongyao Mou, Yaqi Bai, Mengli Xu, Dan Lv, Jinan Deng, Ning Hu, Jun Yang
Summary: This study proposes a microfluidic chip for cell electroporation based on a microtrap array, which optimizes the microtrap structure for single-cell capture and electric field focusing. Compared with a uniform electric field, a lower threshold electric field is required to induce electroporation and produces a higher transmembrane voltage on the cell in the microchip, showing an improvement in cell viability and electroporation efficiency. Furthermore, the larger perforation area produced on the cells in the microchip under a specific electric field allows a higher substance transfer efficiency and is less affected by the cell size, benefiting the consistency of substance transfer.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Sherif Ashraf Fahmy, Asmaa Ramzy, Basma M. Saleh, Hassan Mohamed El-Said Azzazy
Summary: Supramolecular nanovesicles, including pillar[n]arenes, have emerged as potential smart nanocarriers for cancer chemotherapeutics, offering high encapsulation capacity and controlled drug release capabilities. Their use in cancer targeted drug delivery has shown promise due to their symmetric structure, ease of functionalization, and biocompatibility.
Article
Chemistry, Multidisciplinary
Sirun Yang, Dennis Larsen, Maria Pellegrini, Sebastian Meier, Dale F. Mierke, Sophie R. Beeren, Ivan Aprahamian
Summary: The high water solubility of gamma-cyclodextrin can lower synthetic yield and increase production costs, but a photoremovable hydrazone template has been shown to increase efficiency in enzymatic synthesis and reduce costs. The Z isomer of the hydrazone switch is preferentially included in gamma-CD, leading to a 6-fold increase in yield. This discovery may pave the way for designing adaptive materials based on gamma-CD.
Article
Mechanics
Riande I. Dekker, Antoine Deblais, Bastiaan Veltkamp, Peter Veenstra, Willem K. Kegel, Daniel Bonn
Summary: The destabilization of oil-in-water emulsions is usually slow under normal conditions, with the characteristic time scale determined by drainage of the continuous phase and coalescence of the dispersed phase. However, compressing emulsions between two plates can significantly speed up the destabilization process. This acceleration is due to faster drainage caused by squeezing force application and rearrangements from creep processes contributing to the destabilization.
Article
Physics, Applied
Jinsong Guo, Congcong Chen, Zhenglong Sun, Long Zhang, Hui Li, Xiaodong Wu, Jie Zhuang
Summary: Super-resolution fluorescence microscopy (SRFM) revolutionizes biomedical research by providing nanometer-scale information within cells. Recent advances in SRFM enable researchers to probe dynamic processes in living cells with unprecedented resolution. This study uses SRFM to visualize and quantify changes in vesicle trafficking dynamics induced by intense nano-pulse stimulation (NPS) in cancer cells, providing experimental evidence that NPS directly affects vesicle trafficking and potentially sheds light on the mechanism of NPS in inhibiting cancer invasion and metastasis. Additionally, this work suggests a potential physical method to regulate vesicle transport.
APPLIED PHYSICS LETTERS
(2023)
Article
Materials Science, Multidisciplinary
V. Mattos Ferreira, M. G. Mecozzi, R. H. Petrov, J. Sietsma
Summary: The influence of soaking and cooling rates on the final microstructure of a fast-heated railway steel was investigated. The microstructure was characterized and the influence of each condition was detailed. The results can serve as a guideline for replicating White Etching Layers in laboratory conditions and interpreting field conditions.
MATERIALS & DESIGN
(2022)
Article
Chemistry, Multidisciplinary
Hao Chen, Jin Zhang, Wenting Yu, Yi Cao, Zhaozhen Cao, Yebang Tan
Summary: The study demonstrates the control of viscoelasticity of polymer networks by designing crosslinks with variable molecular dynamics, achieving a quantitative control over mechanical properties and reversible mechanical response. This strategy paves the way for engineering smart responsive materials by tailoring the mechanical properties of polymer networks at the molecular level.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Zhiqiang Zhao, Zheng Bian, Yu Chen, Chuanqing Kang, Lianxun Gao, Guangshan Zhu
Summary: The study shows that chiral oligomers can self-assemble into vesicle-like particles in hydrophobic or hydrophilic solvent systems independently of molecular design and chemical environments. The vesicular membranes are likely stabilized by pi-pi stacking interactions between foldamer backbones and collective van der Waals interactions between side chains, as supported by TEM, UV, CD and PXRD data.
Article
Chemistry, Multidisciplinary
Fei Guo, Ji Wang, Jiong Zhou, Kun Qian, Hongchun Qu, Ping Liu, Shidong Zhai
Summary: The effects of phospholipids and cholesterol content on bilayer membrane electroporation were studied using molecular dynamics simulations. The results showed that membrane composition affects the electroporation mechanism and different composition values lead to varying average electroporation time.
Article
Chemistry, Physical
Lucrezia Caselli, Andrea Ridolfi, Jacopo Cardellini, Lewis Sharpnack, Lucia Paolini, Marco Brucale, Francesco Valle, Costanza Montis, Paolo Bergese, Debora Berti
Summary: The study introduces a novel method to quantify the stiffness of vesicles by utilizing the surface plasmon resonance (SPR) of gold nanoparticles adsorbed on the vesicles. This paves the way for developing a cost-effective and efficient method to assess the mechanical properties of nanosized vesicles.
NANOSCALE HORIZONS
(2021)
Article
Chemistry, Analytical
Shan Chen, Tao Jiang, Hao Lin, Junyan Chen, Shuangli Yang, Pengcheng Wang, Xinqiang Gan, Yali Wang, Bin Xu, Junjie Sun, Congcong Yin, Zongxiong Huang, Yimin Fang
Summary: The study successfully detected low-concentration exosomes in complex matrices by enriching exosomes with a filter membrane and using CuS nanoparticles as labels. The high sensitivity, specificity, and simplicity of the method make it suitable for rapid diagnosis of diseases like prostate cancer.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
D. Li, P. Wang, W. W. Zhu, B. Zhang, X. X. Zhang, R. Duan, Y. K. Zhang, Y. Feng, N. Y. Tang, S. Chatterjee, J. M. Cordes, M. Cruces, S. Dai, V Gajjar, G. Hobbs, C. Jin, M. Kramer, D. R. Lorimer, C. C. Miao, C. H. Niu, J. R. Niu, Z. C. Pan, L. Qian, L. Spitler, D. Werthimer, G. Q. Zhang, F. Y. Wang, X. Y. Xie, Y. L. Yue, L. Zhang, Q. J. Zhi, Y. Zhu
Summary: The observation of FRB 121102 reveals a bimodal burst rate energy distribution, the absence of detectable periodicity or quasi-periodicity, challenging models involving a single rotating compact object.
Article
Multidisciplinary Sciences
Lizhi Liu, Dingyi Pan, Sheng Chen, Maria-Viola Martikainen, Anna Karlund, Jing Ke, Herkko Pulkkinen, Hanna Ruhanen, Marjut Roponen, Reijo Kakela, Wujun Xu, Jie Wang, Vesa-Pekka Lehto
Summary: The mechanism underlying partial coating in cell membrane (CM) coating technology has been revealed to be the adsorption of CM fragments or vesicles, which can eventually be ruptured under external force. Partial coating is difficult to self-repair due to limited membrane fluidity. Introducing external phospholipids as a helper can increase CM fluidity and promote the final fusion of lipid patches.
NATURE COMMUNICATIONS
(2022)
Review
Nanoscience & Nanotechnology
Guo Zhang, Jiaming Sun
Summary: Liposomes are widely used in biomedical applications such as drug delivery, and advancements in micro-and nanofabrication techniques have revolutionized their preparation. This review introduces state-of-the-art liposomal formation on microfluidic chips, providing a reference for researchers comparing various microfluidic techniques.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Cerina Chhuon, Luis Vicente Herrera-Marcos, Shao-Yu Zhang, Cecile Charriere-Bertrand, Vincent Jung, Joanna Lipecka, Berkan Savas, Nour Nasser, Andre Pawlak, Hocine Boulmerka, Vincent Audard, Dil Sahali, Ida Chiara Guerrera, Mario Ollero
Summary: This study compared different methods of plasma preparation, analyzed the plasma proteomes of patients with recurrent focal and segmental glomerulosclerosis (FSGS) and controls, and investigated the effects of patient plasma on podocytes. The results showed that proteins involved in neutrophil degranulation were significantly increased in the plasma of FSGS patients, while lipid-binding proteins associated with lipoproteins were decreased. Additionally, amino acid transporters involved in mTORC1 activation were found to be significantly increased in extracellular vesicles (EV) from FSGS patients. Furthermore, the study revealed profound differences in proteins involved in the mTOR pathway, autophagy, and cytoskeleton organization in podocytes exposed to patient plasma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Mathilde Coustets, Caroline Ladurantie, Elisabeth Bellard, Melissa Prat, Marie-Pierre Rols, Vincent Ecochard, Gwenael Ferron, Sophie Chabot, Muriel Golzio, Laurent Paquereau
Article
Biochemistry & Molecular Biology
Sara Gouarderes, Layal Doumard, Patricia Vicendo, Anne-Francoise Mingotaud, Marie-Pierre Rols, Laure Gibot
BIOELECTROCHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Sebastjan Nemec, Slavko Kralj, Claire Wilhelm, Ali Abou-Hassan, Marie-Pierre Rols, Jelena Kolosnjaj-Tabi
APPLIED SCIENCES-BASEL
(2020)
Editorial Material
Oncology
Marie-Pierre Rols, Muriel Golzio, Jelena Kolosnjaj-Tabi
Article
Biochemistry & Molecular Biology
Jelena Kolosnjaj-Tabi, Muriel Golzio, Elisabeth Bellard, Alexandre Catrain, Thomas Chretiennot, Quentin Saurin, Jacques Tarayre, Rene Vezinet, Marie-Pierre Rols
Summary: The study demonstrated that exposure to both narrow-band and wide-band high-power electromagnetic waves had no significant impact on the growth of healthy and tumor-bearing animals, as well as on the growth of murine skin tumors. However, wide-band signals may cause behavioral changes in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sandrine Pelofy, Hugo Bousquet, Laure Gibot, Marie-Pierre Rols, Muriel Golzio
Summary: The ability to modulate deregulated genes by RNAi shows promise for treating certain diseases, including cancers. While in vitro studies have shown the direct transfer of negatively charged siRNA across the plasma membrane, understanding the efficiency of siRNA electrotransfer into 3D tissues remains a challenge. Nonetheless, the asymmetrical distribution of siRNA within multicellular spheroids is a key finding and suggests that spheroids could be a valuable alternative to animal experimentation.
BIOELECTROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Lynn Carr, Muriel Golzio, Rosa Orlacchio, Geraldine Alberola, Jelena Kolosnjaj-Tabi, Philippe Leveque, Delia Arnaud-Cormos, Marie-Pierre Rols
Summary: The study found that nanosecond pulsed electric field (nsPEF) had strong biological effects on the spheroid tumor model of human colorectal carcinoma cells, including increased membrane permeabilization and cellular death. The biological response increased significantly with the number of pulses applied.
BIOELECTROCHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Juliette Simon, Bastien Jouanmiqueou, Marie-Pierre Rols, Emmanuel Flahaut, Muriel Golzio
Summary: This study demonstrated the potential of non-invasive transdermal drug delivery using a novel electrode/reservoir material, revealing an inherent asymmetry in permeation pathways generated in the stratum corneum during treatment. Additionally, the study validated a model molecule to optimize electrical parameters for improved macromolecule delivery.
Article
Pharmacology & Pharmacy
L. Simon, E. Bellard, B. Jouanmiqueou, V. Lapinte, N. Marcotte, J. M. Devoisselle, C. Lamaze, M. P. Rols, M. Golzio, S. Begu
Summary: In this study, the potential of amphiphilic polyoxazolines (POx) to interact with biological membranes was evaluated. The study found that POx has excellent penetration into lipid bilayers and cells, and its interactions with membranes depend on the nature of the alkyl chain constituting the POx. Both saturated and unsaturated POx types were efficiently accumulated in the stratum corneum barrier of mouse skin, showing potential for topical delivery of active molecules.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Engineering, Biomedical
Amar Tamra, Amel Zedek, Marie-Pierre Rols, David Dubuc, Katia Grenier
Summary: This paper presents a 40 GHz microwave biosensor used to monitor and characterize single cells subjected to electrochemotherapy. The biosensor offers a non-destructive and label-free technique to extract dielectric parameters and detect the effects of chemical treatments. The results show a high correlation rate and demonstrate the reliability and efficacy of the technique for biology research and personalized medicine.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Aurelie Marches, Emily Clement, Geraldine Alberola, Marie-Pierre Rols, Sarah Cousty, Michel Simon, Nofel Merbahi
Summary: This study suggests that helium-based CAP treatments can improve wound healing by stimulating keratinocyte migration. Long-term CAP treatments are cytotoxic, reducing keratinocyte migration and inducing oxidative stress. Short-term CAP treatments are not cytotoxic, do not affect keratinocyte proliferation and differentiation, and accelerate wound closure by improving keratinocyte migration in vitro.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Radiology, Nuclear Medicine & Medical Imaging
Jean-Pierre Tasu, David Tougeron, Marie-Pierre Rols
Summary: Thermal tumor ablation techniques have limitations due to heat sink effect, while electroporation opens new possibilities for tumor ablation by inducing cell death and enabling drug delivery.
DIAGNOSTIC AND INTERVENTIONAL IMAGING
(2022)
Editorial Material
Pharmacology & Pharmacy
Elisabeth Garanger, Nathalie Mignet, Chantal Pichon, Marie-Pierre Rols, Lucie Sancey
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Marie Beaurain, Franck Talmont, Damien Pierre, Patrice Peran, Samuel Boucher, Anne Hitzel, Marie-Pierre Rols, Olivier Cuvillier, Pierre Payoux, Salabert Anne-Sophie
Summary: Dysfunction of NMDA receptors is central to the pathophysiology of psychiatric and neurodegenerative disorders, but its mechanisms are not well understood. This study describes the development of a PET tracer that can selectively bind to the NMDA receptors and visualize them in an open and active state. It also demonstrates the tracer's ability to detect activated NMDA receptors in a rat model of excitotoxicity.
MOLECULAR IMAGING AND BIOLOGY
(2023)
Article
Engineering, Biomedical
Annabelle Collin, Hadrien Bruhier, Jelena Kolosnjaj, Muriel Golzio, Marie-Pierre Rols, Clair Poignard
Summary: The objective of this study was to investigate the growth specificities of cancer cell spheroids under pulsed electric field. Through experiments and mathematical modeling, the growth of the spheroids was characterized and it was found that the electric field intensity had an impact on the growth. Partially irreversible electric pulses were shown to accelerate the regrowth of the spheroids.
AIMS BIOENGINEERING
(2022)