Article
Chemistry, Physical
Yunong Zhao, Jing Huang, Qing Huang, Yanbing Tao, Ruiqin Gu, Hua-Yao Li, Huan Liu
Summary: In this study, a sensitive glucose electrochemical biosensor was designed using PbS colloidal quantum dots and Au nanospheres as labels. The biosensor demonstrated high sensitivity and stability, and showed a linear response in a wide range of glucose concentrations.
Article
Immunology
Masayuki Morita, Bernard N. Kanoi, Naoaki Shinzawa, Rie Kubota, Hiroyuki Takeda, Tatsuya Sawasaki, Takafumi Tsuboi, Eizo Takashima
Summary: The study developed a novel system using AGIA tag and anti-AGIA monoclonal antibody, successfully localizing proteins in blood-stage malaria parasites, which could potentially be a useful tool for elucidating the subcellular localization of new and understudied proteins.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Chemistry, Physical
David Doan, Daniel J. Echeveste, John Kulikowski, X. Wendy Gu
Summary: Optical and confocal microscopy are used to image the self-assembly of microscale colloidal particles. Machine learning is applied to improve identification speed and accuracy, achieving a mean Average Precision (mAP) of around 85%. Synthetic data generated using Blender supplements the training data, capturing key characteristics of confocal images.
Article
Optics
Gwiyeong Moon, Taehwang Son, Hajun Yoo, Changhun Lee, Hyunwoong Lee, Seongmin Im, Donghyun Kim
Summary: The interaction between single emitters and plasmonic structures can enhance optical properties but also causes distortion. By studying axially defocused images, the distortion can be explored and the precise positions of emitters can be retrieved, leading to improved localization accuracy.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Chemistry, Physical
Pejman Ganjeh-Anzabi, Heidi Jahandideh, Stephanie A. Kedzior, Milana Trifkovic
Summary: A reliable method for determining the surface free energy (SFE) of nanoparticles has been developed in this study. The method takes into account the effect of surface roughness on the measurements and has been verified experimentally. The SFE of various materials has been successfully determined using this method.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Multidisciplinary Sciences
Roko Duplancic, Darko Kero
Summary: This novel approach allows for quantification and colocalization of multiple markers on high-resolution panoramic images of histological sections using standard staining techniques and readily available software. It avoids the limitations of traditional cell counting and ROI selection by quantifying expression domains and gradients of IF signals.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Ioanna Tremi, Sophia Havaki, Sofia Georgitsopoulou, Nefeli Lagopati, Vasilios Georgakilas, Vassilis G. Gorgoulis, Alexandros G. Georgakilas
Summary: The article discusses the combined effects of ionizing radiation with high-Z metallic nanoparticles, particularly gold, on DNA damage. It highlights the use of transmission electron microscopy (TEM) to study these effects at a detailed cellular level, focusing on the uptake and damage of nanoparticles in cells. The goal is to better understand the radiosensitization mechanisms of nanoparticles.
Article
Chemistry, Physical
Nicola Peruffo, Fabrizio Mancin, Elisabetta Collini
Summary: The ultrafast dynamics of plexcitonic materials, influenced by other states, have longer time constants than typical values found in literature and can be modulated, opening up potential prospects for modifying rates of chemical reactions.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Cardiac & Cardiovascular Systems
Julius Bogomolovas, Zengming Zhang, Tongbin Wu, Ju Chen
Summary: The use of digital image analysis and count regression models improves the reproducibility and rigor of cardiovascular research in histological studies. Formalized computer-based quantification strategies remove researcher bias, allow for higher analysis throughput, and facilitate sharing of quantification tools, enhancing research transparency and data transferability.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Franziska Weigl, Carina Blum, Ana Sancho, Juergen Groll
Summary: In recent decades, hybrid characterization systems have become important in cellular biomechanics research. This paper introduces a multifunctional device that combines Fluorescence Microscopy (FL) with FluidFM technology, allowing for real-time optical tracking of cell detachment processes while acquiring force-distance curves simultaneously.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Engineering, Mechanical
Zuo Zhu, Siu-Kui Au
Summary: This study investigates the posterior uncertainty of modal parameters and the computation of the posterior covariance matrix, building upon previous research in Bayesian operational modal analysis.
MECHANICAL SYSTEMS AND SIGNAL PROCESSING
(2022)
Article
Chemistry, Physical
Andrew Nelson, Lawrence H. Friedman
Summary: The true thermodynamic stability of solid colloidal dispersions is usually unexpected and not thoroughly validated. In this study, classical nucleation theory (CNT) is applied to these systems and a relationship between interfacial free energy density and particle size distribution is established. The experimental data of gold-thiol and magnetite nanoparticles can be well described using this relationship. The study highlights discrepancies with previous models and provides insights for future research.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Chemistry, Multidisciplinary
Andreas Karg, Tamino Roessler, Andreas Mark, Paul Markus, Tobias Lauster, Nicolas Helfricht, Georg Papastavrou
Summary: The colloidal probe technique, based on micrometer-sized colloidal particles attached to a cantilever, revolutionized direct force measurements by AFM. The versatile approach presented allows combining direct force measurements and potentiostatic control, enabling the study of adhesion behavior and long-range forces of electrode materials. The resulting interaction force profiles are in good agreement with theoretical calculations based on charge regulation and solutions of the Poisson-Boltzmann equation.
Article
Computer Science, Information Systems
Christian Ritter, Roman Thielemann, Ji-Young Lee, Minh Tu Pham, Ralf Bartenschlager, Karl Rohr
Summary: We introduce a software suite, consisting of ColocQuant and ColocJ, for colocalization analysis of spot-like objects in multi-channel fluorescence microscopy images. It provides efficient quantification and intuitive visualization of colocalization in two or three channels, as well as global statistics of particle properties. The software has been successfully applied to analyze live cell fluorescence microscopy image sequences of viral proteins in hepatitis C virus infected cells.
Article
Biochemistry & Molecular Biology
Anton S. Shakhov, Polina A. Kovaleva, Alexandra S. Churkina, Igor I. Kireev, Irina B. Alieva
Summary: Actin cytoskeleton is crucial for cellular processes, and there are different isoforms in non-muscle cells, β-actin and γ-actin, which have distinct functions and localization in the cytoplasm. Based on super-resolution microscopy, this study demonstrates that β-actin and γ-actin are segregated in the cytoplasm of endothelial cells, with varying degrees of colocalization in different cellular regions. The segregation of actin isoforms is enhanced during the activation of cell lamella in endothelial barrier dysfunction.
Article
Biochemistry & Molecular Biology
Michael J. Munson, George F. G. Allen, Rachel Toth, David G. Campbell, John M. Lucocq, Ian G. Ganley
Article
Cell Biology
Mohammed Arastoo, Christian Hacker, Petra Popovics, John M. Lucocq, Alan J. Stewart
HISTOCHEMISTRY AND CELL BIOLOGY
(2016)
Review
Anatomy & Morphology
Terry M. Mayhew, John M. Lucocq
JOURNAL OF ANATOMY
(2015)
Review
Cell Biology
John M. Lucocq, Terry M. Mayhew, Yannick Schwab, Anna M. Steyer, Christian Hacker
TRENDS IN CELL BIOLOGY
(2015)
Article
Multidisciplinary Sciences
Christian Hacker, Jalal Asadi, Christos Pliotas, Sophie Ferguson, Lee Sherry, Phedra Marius, Javier Tello, David Jackson, James Naismith, John Milton Lucocq
SCIENTIFIC REPORTS
(2016)
Review
Cell Biology
Sophie Ferguson, Anna M. Steyer, Terry M. Mayhew, Yannick Schwab, John Milton Lucocq
HISTOCHEMISTRY AND CELL BIOLOGY
(2017)
Article
Microscopy
Jalal Asadi, Sophie Ferguson, Hussain Raja, Christian Hacker, Phedra Marius, Richard Ward, Christos Pliotas, James Naismith, John Lucocq
Article
Multidisciplinary Sciences
Sven-A. Freibert, Alina V. Goldberg, Christian Hacker, Sabine Molik, Paul Dean, Tom A. Williams, Sirintra Nakjang, Shaojun Long, Kacper Sendra, Eckhard Bill, Eva Heinz, Robert P. Hirt, John M. Lucocq, T. Martin Embley, Roland Lill
NATURE COMMUNICATIONS
(2017)
Article
Multidisciplinary Sciences
Alistair Elfick, Grigore Rischitor, Rabah Mouras, Asim Azfer, Lisa Lungaro, Marc Uhlarz, Thomas Herrmannsdoerfer, John Lucocq, Wesam Gamal, Pierre Bagnaninchi, Scott Semple, Donald M. Salter
SCIENTIFIC REPORTS
(2017)
Article
Biophysics
Richard Ward, Christos Pliotas, Emma Branigan, Christian Hacker, Akiko Rasmussen, Gregor Hagelueken, Ian R. Booth, Samantha Miller, John Lucocq, James H. Naismith, Olav Schiemann
BIOPHYSICAL JOURNAL
(2014)
Article
Cell Biology
Christian Hacker, Matthew Howell, David Bhella, John Lucocq
CELLULAR MICROBIOLOGY
(2014)
Article
Microbiology
Sophie Ferguson, John Lucocq
Article
Biochemistry & Molecular Biology
Yvonne Giesecke, Samuel Soete, Katarzyna MacKinnon, Thanasis Tsiaras, Madeline Ward, Mohammed Althobaiti, Tamas Suveges, James E. Lucocq, Stephen J. McKenna, John M. Lucocq
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Clinical Neurology
Marzena Kurzawa-Akanbi, Seshu Tammireddy, Ivo Fabrik, Mary K. Doherty, Rachel Heap, Bjorn M. Burmann, Matthias Trost, John M. Lucocq, Anda Gherman, Graham Fairfoul, Preeti Singh, Florence Burte, Alison Green, Ian G. McKeith, Anetta Hartlova, Phillip D. Whitfield, Christopher M. Morris, Lina Gliaudelyte, Irena Matetko-Burmann
Summary: Mutations in glucocerebrosidase (GBA) are the most prevalent genetic risk factor for Lewy body disorders (LBD), characterized by altered sphingolipid metabolism in LBD regardless of GBA mutations. Extracellular vesicles (EV) derived from LBD cerebrospinal fluid and brain tissue are loaded with ceramides and neurodegeneration-linked proteins, capable of inducing aggregation of wild-type alpha-synuclein. This suggests abnormalities in ceramide metabolism are a feature of LBD and GBA mutations likely accelerate the pathological process in sporadic LBD.
ACTA NEUROPATHOLOGICA
(2021)
Article
Cell Biology
Jose M. Serra Lleti, Anna M. Steyer, Nicole L. Schieber, Beate Neumann, Christian Tischer, Volker Hilsenstein, Mike Holtstrom, David Unrau, Robert Kirmse, John M. Lucocq, Rainer Pepperkok, Yannick Schwab
Summary: This study presents a toolset for adherent cells that enables tracking and finding cells in FIB-SEM, along with automatic acquisition of high-resolution volume datasets. The workflow is completely automated using computer vision techniques, reducing or eliminating operator supervision.
JOURNAL OF CELL BIOLOGY
(2022)