Article
Oncology
Michela Corsini, Cosetta Ravelli, Elisabetta Grillo, Patrizia Dell'Era, Marco Presta, Stefania Mitola
Summary: The study developed a novel experimental model to investigate vessel formation during tumor growth by grafting murine ESCs onto the chick embryo CAM, demonstrating the occurrence of both vasculogenic and angiogenic events in the tumor mass.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Magdalena K. Sznurkowska, Nicola Aceto
Summary: Metastasis is a major cause of cancer-related deaths, involving tumor expansion, intravasation of cancer cells into the circulation, survival in the bloodstream, extravasation at distant sites, and organ colonization. The process of intravasation, where cancer cells leave the primary tumor site, plays a crucial role in initiating the metastatic cascade. Various factors, such as intrinsic properties of cancer cells, tumor microenvironment, and mechanical cues, are involved in this process.
Article
Biochemistry & Molecular Biology
Selvaraj Vimalraj, Suresh Renugaa, Anuradha Dhanasekaran
Summary: This paper introduces the chick embryo chorioallantoic membrane as a platform for evaluating the biological characteristics of biomaterials, and explores its potential applications in the field of tissue engineering.
PROCESS BIOCHEMISTRY
(2023)
Article
Oncology
Kelly Harper, Anna Yatsyna, Martine Charbonneau, Karine Brochu-Gaudreau, Alexis Perreault, Claudio Jeldres, Patrick P. McDonald, Claire M. Dubois
Summary: Hypoxia in the tumor microenvironment is a negative prognostic factor associated with tumor progression and metastasis, making it an attractive therapeutic target for anti-tumor therapy. The use of the CAM model effectively supports the development of hypoxic zones in a variety of tumor types, offering a valuable platform for studying hypoxia and testing drugs targeting this tumor microenvironment.
Article
Medicine, General & Internal
Lucia Borriello, John Condeelis, David Entenberg, Maja H. Oktay
Summary: The research found that breast cancer cells can disseminate not only from primary tumors and metastatic nodules in the lymph nodes to distant organs, but also from lung metastases. Re-dissemination of cancer cells could be a crucial mechanism leading to overt metastases and patient demise.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Oncology
Fani Karagianni, Christina Piperi, Berta Casar, Dalia de la Fuente-vivas, Rocio Garcia-Gomez, Kyriaki Lampadaki, Vasiliki Pappa, Evangelia Papadavid
Summary: The combination of Resminostat and Ruxolitinib demonstrated significant antitumor effects in CTCL progression, inhibiting tumor growth, migration, invasion, and metastasis. The chick embryo chorioallantoic membrane (CAM) model was used to validate these effects, providing a preclinical in vivo model for pharmacological testing.
Article
Multidisciplinary Sciences
Samuel V. Rasmussen, Noah E. Berlow, Lisa Hudson Price, Atiya Mansoor, Stefano Cairo, Sandra Rugonyi, Charles Keller
Summary: Preclinical cancer research can be expensive, with in vitro studies often not representative of the tumor microenvironment and mouse studies being costly at scale. Using Japanese quail in chorioallantoic membrane (CAM) assays, researchers have developed a cost-effective method to screen for anti-cancer efficacy and drug toxicity, demonstrating concordance with murine in vivo studies and suggesting potential for validating new anti-cancer agents.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Colmar Hilbrig, Jessica Loeffler, Gabriel Fischer, Ellen Scheidhauer, Christoph Solbach, Markus Huber-Lang, Ambros J. J. Beer, Volker Rasche, Gordon Winter
Summary: The CAM model shows promise as an alternative to mouse models in terms of the 3Rs principles. However, further evaluation is needed to determine its value in noninvasive assessment of radiolabeled macromolecules using PET and MRI. This study analyzed the biodistribution and tumor accumulation of Zr-89-labeled albumin in both CAM and mouse models, and found no significant differences in albumin influx kinetics between the two models. These findings suggest that the CAM model could be a potential alternative for initial PET studies on the characteristics of EPR-dependent target accumulation of radiolabeled macromolecules.
Article
Oncology
Jessica Loeffler, Hendrik Herrmann, Ellen Scheidhauer, Mareike Wirth, Anne Wasserloos, Christoph Solbach, Gerhard Glatting, Ambros J. Beer, Volker Rasche, Gordon Winter
Summary: This study used the chorioallantoic membrane (CAM) model to assess the target-specific binding of radiopharmaceuticals and investigated the feasibility of inhibition studies in the CAM model. The results demonstrated that the CAM model can be used to detect the receptor-specific binding of radiopharmaceuticals. This study provides an alternative to mouse experiments for early evaluation of promising radiopharmaceuticals.
Editorial Material
Oncology
Nicola Aceto
Summary: The metastatic process is a complex procedure, similar to the migratory patterns of humans or animals on Earth. Studying circulating tumor cells (CTCs) has revealed their characteristics and mechanisms in forming metastasis, providing opportunities for the development of anti-metastasis medicines.
MOLECULAR ONCOLOGY
(2023)
Article
Cell Biology
Wenjun Wang, Paul V. Taufalele, Martial Millet, Kevin Homsy, Kyra Smart, Emily D. Berestesky, Curtis T. Schunk, Matthew M. Rowe, Francois Bordeleau, Cynthia A. Reinhart-King
Summary: This study reveals that matrix stiffness promotes tumor cell intravasation by increasing MENA expression and decreasing ESRP1 expression, leading to enhanced contractility and intravasation.
Article
Toxicology
Alessandro Maugeri, Giovanni E. Lombardo, Michele Navarra, Santa Cirmi, Antonio Rapisarda
Summary: This study aimed to optimize data extrapolation from CAM assay, leading to greater reliability and richness of data for evaluating potential pro- and anti-angiogenic activity of new candidate drugs, while saving eggs and time for experiments.
JOURNAL OF APPLIED TOXICOLOGY
(2022)
Article
Oncology
Margherita Pergolizzi, Laura Bizzozero, Federica Maione, Elena Maldi, Claudio Isella, Marco Macagno, Elisa Mariella, Alberto Bardelli, Enzo Medico, Caterina Marchio, Guido Serini, Federica Di Nicolantonio, Federico Bussolino, Marco Arese
Summary: This study revealed that NLGN1 is expressed in human colorectal tumors and promotes CRC cell migration and metastasis. Mechanistically, NLGN1 promotes the localization of APC to the cell membrane, stimulates beta-catenin translocation to the nucleus, and induces an EMT phenotype in CRC cell lines. This novel finding suggests NLGN1 as a potential therapeutic target for CRC.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Pan Zhang, Xing Lai, Mao-Hua Zhu, Mei Long, Xue-Liang Liu, Zi-Xiang Wang, Yifan Zhang, Run-Jie Guo, Jing Dong, Qin Lu, Peng Sun, Chao Fang, Mei Zhao
Summary: Saikosaponin A (SSA) has been shown to suppress proliferation, migration, and tube formation of endothelial cells, as well as inhibit angiogenesis in various models. It was found to inhibit tumor growth by blocking the VEGFR2-mediated signaling pathway.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Oncology
Chen Chen, Yang Liu, Lin Liu, Chaohua Si, Yanxin Xu, Xiaoke Wu, Chengzeng Wang, Zhenqiang Sun, Qiaozhen Kang
Summary: This study found that exosomes derived from colorectal cancer cells carry circTUBGCP4, which induces tip cell formation in vascular endothelial cells, promoting angiogenesis and tumor metastasis through the activation of the Akt signaling pathway.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)