Review
Gastroenterology & Hepatology
Mohamed El-Kassas, Abeer Awad
Summary: Chronic hepatitis C virus (HCV) infection is associated with multiple metabolic factors that can impact the course of the illness and the progression of liver and non-liver-related diseases.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Chemistry, Multidisciplinary
Shirong Wang, Lin Zha, Xin Cui, Yu-Te Yeh, Ruochuan Liu, Jia Jing, Huidong Shi, Weiping Chen, John Hanover, Jun Yin, Liqing Yu, Bingzhong Xue, Hang Shi
Summary: DNA methylation plays an important role in regulating hepatic lipid metabolism. High-fat diet induces increased DNA methylation at the Klb promoter, leading to decreased Klb expression and hepatic steatosis. Targeted demethylation at the Klb promoter can increase Klb expression and fatty acid oxidation, resulting in reduced lipid accumulation.
Review
Gastroenterology & Hepatology
Yi-Wen Shi, Rui-Xu Yang, Jian-Gao Fan
Summary: This review examines the overlapping prevalence of metabolic disorders and CHB, assesses the risk of advanced fibrosis/hepatocellular carcinoma in CHB patients with NAFLD, and discusses remaining clinical issues. Addressing concomitant NAFLD through lifestyle management and disease screening is crucial for better outcomes in CHB patients, and further research is needed for the assessment of progressive changes and novel therapies in these patients.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mosleh Abomughaid, Enoch S. E. Tay, Russell Pickford, Chandra Malladi, Scott A. Read, Jens R. Coorssen, Brian S. Gloss, Jacob George, Mark W. Douglas
Summary: HCV infection increases neutral lipid and phospholipids in cells and induces the expression of PEMT, a key enzyme promoting lipid accumulation and virus replication. The induction of PEMT may explain the genotype-specific differences in hepatic steatosis caused by the virus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Xiaoxia Wang, Yu Cao, Jia Guo, Dezhao Li, Haitao Zhang, Qingkun Song, Jun Lu
Summary: This study found a significant positive association between HCV genotype 3 and the MTTP genotype (-493G/T) with regard to hepatic steatosis, suggesting that the T allele mutation of the MTTP genotype (-493G/T) increases susceptibility to hepatic steatosis in patients with HCV genotype 3.
LIPIDS IN HEALTH AND DISEASE
(2023)
Article
Endocrinology & Metabolism
Yujin Ding, Guangdi Li, Zhiguang Zhou, Tuo Deng
Summary: Diabetes and hepatitis C are two distinct diseases, but they are closely related, as hepatitis C virus infection may lead to diabetes and influence the occurrence and development of diabetes through multiple mechanisms.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Article
Gastroenterology & Hepatology
Saleh A. Alqahtani, Rose Chami, Dua Abuquteish, Shannon M. Vandriel, Charesse Yap, Liyana Kukkadi, Aishwarya Parmar, Amrita Mundh, Eve A. Roberts, Binita M. Kamath, Iram Siddiqui
Summary: This study compared the histological features of Wilson disease (WD) in children with non-alcoholic fatty liver disease (NAFLD) and autoimmune hepatitis (AIH). It found that there was considerable overlap in the light microscopic findings, but ultrastructural findings of mitochondrial abnormalities were important in distinguishing WD from NAFLD and AIH.
LIVER INTERNATIONAL
(2022)
Article
Virology
Yujiao He, Maolin Huang, Chunyan Tang, Yan Yue, Xiao Liu, Zhebin Zheng, Hongbo Dong, Deming Liu
Summary: MicroRNA-122 plays a critical role in HCV replication, and daidzein inhibits HCV replication by decreasing miR-122 levels, enhancing the antiviral effect of IFN-?, without significantly affecting cell growth.
Review
Medicine, General & Internal
Chen-Hua Liu, Jia-Horng Kao
Summary: Hepatitis C virus (HCV) is a significant health problem in hemodialysis patients, and noninvasive indices are being evaluated to diagnose the stage of hepatic fibrosis in HCV-infected patients undergoing hemodialysis.
Review
Virology
Alex S. Hartlage, Amit Kapoor
Summary: The development of viral vaccines should be conducted with a rigorous scientific approach, including initial in-depth design, preclinical evaluation of immunogenicity and safety, and subsequent human clinical trials. Despite past setbacks in preventing chronic infection with an HCV vaccine, ongoing discussions are fueling the need for new approaches.
Review
Biochemistry & Molecular Biology
Wesal Elgretli, Tianyan Chen, Nadine Kronfli, Giada Sebastiani
Summary: Hepatitis C virus (HCV) infection is the main cause of chronic liver disease and can lead to various hepatic diseases. It is also associated with extrahepatic manifestations such as immune-related disorders and metabolic abnormalities. HCV is dependent on the host's lipid metabolism for successful replication.
Article
Virology
Birthe Reinecke, Nicola Frericks, Chris Lauber, Katja Dinkelborg, Alina Matthaei, Florian W. R. Vondran, Patrick Behrendt, Sibylle Haid, Richard J. P. Brown, Thomas Pietschmann
Summary: The liver is an immunoprivileged organ with enhanced resistance to immune responses. This study shows that CD302 contributes to liver cell-intrinsic defense against HCV and might mediate broader antiviral defenses against additional hepatotropic viruses.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Amit Kumar Mishra, Md Musa Hossain, Teja Naveen Sata, Ajay K. Yadav, Shahidullah Zadran, Amrendra Kumar Sah, Baibaswata Nayak, Senthil Kumar Venugopal
Summary: HBV infection inhibits the expression of host restriction factor BANF1, partly through upregulation of miR-203 mediated by HBx, which contributes to viral replication and survival.
MICROBIOLOGY SPECTRUM
(2023)
Article
Gastroenterology & Hepatology
Feng Chen, Yingxia Liu, Qianhui Li, Fei Wang
Summary: This study found that there is a common activation of NLRP3 inflammasome between chronic hepatitis B (CHB) and non-alcoholic fatty liver disease/non-alcoholic steatohepatitis (NAFLD/NASH). Inhibiting NLRP3 inflammasome may be an effective strategy for the treatment of CHB and NASH.
CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Qi Han, Huiling Chen, Likai Wang, Yang An, Xiaoxiang Hu, Yaofeng Zhao, Hao Zhang, Ran Zhang
Summary: Research on the relationship between GHR mutation and lipid metabolism in GHR KO pigs and siGHR-treated human liver cells showed that GHR loss disrupts the ERK-AHR-ACOX1/CPT1A pathway, leading to hepatic steatosis. AHR has been identified as a key modulator of lipid metabolism disorder and a potential therapeutic target for hepatic steatosis. The study sheds light on the molecular mechanisms underlying lipid metabolism abnormalities in LS and provides new insights for developing treatments.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Ragunath Singaravelu, Prashanth Srinivasan, John Paul Pezacki
CANADIAN JOURNAL OF MICROBIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Ragunath Singaravelu, Shifawn O'Hara, Daniel M. Jones, Ran Chen, Nathan G. Taylor, Prashanth Srinivasan, Curtis Quan, Dominic G. Roy, Rineke H. Steenbergen, Anil Kumar, Rodney K. Lyn, Dennis Ozcelik, Yanouchka Rouleau, My-Anh Nguyen, Katey J. Rayner, Tom C. Hobman, David Lorne Tyrrell, Rodney S. Russell, John Paul Pezacki
NATURE CHEMICAL BIOLOGY
(2015)
Article
Chemistry, Medicinal
Ragunath Singaravelu, Genevieve F. Desrochers, Prashanth Srinivasan, Shifawn O'Hara, Rodney K. Lyn, Rolf Mueller, Daniel M. Jones, Rodney S. Russell, John Paul Pezacki
ACS INFECTIOUS DISEASES
(2015)
Article
Multidisciplinary Sciences
Ragunath Singaravelu, Curtis Quan, Megan H. Powdrill, Tyler A. Shaw, Prashanth Srinivasan, Rodney K. Lyn, Rhea C. Alonzi, Daniel M. Jones, Roxana Filip, Rodney S. Russell, John P. Pezacki
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Muneaki Nakamura, Prashanth Srinivasan, Michael Chavez, Matthew A. Carter, Antonia A. Dominguez, Marie La Russa, Matthew B. Lau, Timothy R. Abbott, Xiaoshu Xu, Dehua Zhao, Yuchen Gao, Nathan H. Kipniss, Christina D. Smolke, Joseph Bondy-Denomy, Lei S. Qi
NATURE COMMUNICATIONS
(2019)
Review
Cell & Tissue Engineering
Mathieu J. F. Crupi, John C. Bell, Ragunath Singaravelu
Article
Biochemistry & Molecular Biology
Ragunath Singaravelu, Nadine Ahmed, Curtis Quan, Prashanth Srinivasan, Christopher J. Ablenas, Dominic G. Roy, John Paul Pezacki
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Prashanth Srinivasan, Christina D. Smolke
NATURE COMMUNICATIONS
(2019)
Review
Biotechnology & Applied Microbiology
Adrian Pelin, Stephen Boulton, Levi A. Tamming, John C. Bell, Ragunath Singaravelu
EXPERT OPINION ON BIOLOGICAL THERAPY
(2020)
Review
Oncology
Taha Azad, Reza Rezaei, Abera Surendran, Ragunath Singaravelu, Stephen Boulton, Jaahnavi Dave, John C. Bell, Carolina S. Ilkow
Article
Multidisciplinary Sciences
Prashanth Srinivasan, Christina D. Smolke
Article
Multidisciplinary Sciences
Prashanth Srinivasan, Christina D. Smolke
Summary: Microbial biosynthesis of plant natural products can provide valuable medicinal compounds. However, metabolite transport limitations pose challenges in reconstructing complex plant pathways in unicellular hosts. This study demonstrates that alleviating transport limitations in an engineered pathway can increase tropane alkaloid production and enable screening of useful derivatives. Incorporating plant transporters, cofactor regeneration mechanisms, and optimized growth conditions into a yeast platform significantly improves de novo production of hyoscyamine and scopolamine. Additionally, computational tools for biosynthetic pathway prediction can be used to produce diverse tropane alkaloid derivatives from simple precursors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Stephen Boulton, Joanna Poutou, Nikolas T. Martin, Taha Azad, Ragunath Singaravelu, Mathieu J. F. Crupi, Taylor Jamieson, Xiaohong He, Ricardo Marius, Julia Petryk, Christiano Tanese de Souza, Bradley Austin, Zaid Taha, Jack Whelan, Sarwat T. Khan, Adrian Pelin, Reza Rezaei, Abera Surendran, Sarah Tucker, Emily E. F. Fekete, Jaahnavi Dave, Jean-Simon Diallo, Rebecca Auer, Jonathan B. Angel, D. William Cameron, Jean-Francois Cailhier, Rejean Lapointe, Kyle Potts, Douglas J. Mahoney, John C. Bell, Carolina S. Ilkow
Summary: This study evaluates the effects of dose escalation and administration routes on vaccine safety, efficacy, and immunogenicity in animal models. The developed vaccine platform shows robust immune responses and protective immunity against SARS-CoV-2 variants, offering an alternative or complementary approach to current vaccines.
Article
Multidisciplinary Sciences
Taha Azad, Reza Rezaei, Ragunath Singaravelu, Adrian Pelin, Stephen Boulton, Julia Petryk, Kemal Alper Onsu, Nikolas T. Martin, Victoria Hoskin, Mina Ghahremani, Marie Marotel, Ricardo Marius, Xiaohong He, Mathieu J. F. Crupi, Huy-Dung Hoang, Abolfazl Nik-Akhtar, Mahsa Ahmadi, Nika Kooshki Zamani, Ashkan Golshani, Tommy Alain, Peter Greer, Michele Ardolino, Bryan C. Dickinson, Lee-Hwa Tai, Carolina S. Ilkow, John C. Bell
Summary: The authors present chemogenetic switches adapted for vaccinia virus vectors, which allow temporal and dose control of viral replication and transgene expression, as well as the implementation of complex tunable transgene circuitry. These switches enable precise modulation of transgene circuitry in oncolytic virus design and improve the safety and efficacy of vaccinia virus vectors.
NATURE COMMUNICATIONS
(2023)