Article
Biochemistry & Molecular Biology
Sam Seok Cho, Ji Hye Yang, Ji Hyun Lee, Jin Sol Baek, Sae Kwang Ku, Il Je Cho, Kyu Min Kim, Sung Hwan Ki
Summary: The study suggests that ferroptosis contributes to the progression of hepatic fibrosis by activating hepatic stellate cells and increasing the expression of fibrosis markers.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Floris Haijer, Shiva Koets-Shajari, Janette Heegsma, Sandra Serna-Salas, Tjasso Blokzijl, Manon Buist-Homan, Han Moshage, Klaas Nico Faber
Summary: Liver fibrosis is caused by excessive proliferation and collagen production by hepatic stellate cells (HSCs) due to chronic liver injury. Hydroxyurea, an anti-proliferative drug, showed inhibitory effects on HSC proliferation and fibrosis development in both in vitro and in vivo experiments. This study provides evidence for the therapeutic potential of hydroxyurea in treating liver fibrosis.
Review
Cell Biology
Richa Rani, Chandrashekhar R. Gandhi
Summary: Perisinusoidal hepatic stellate cells (HSCs) are extensively studied for their role as the main fibrogenic cells in chronic liver injury. HSCs produce various cytokines, chemokines, and growth mediators, and express cell adhesion molecules continuously and in response to stimuli such as endotoxin. Through interaction with immune and inflammatory cells, HSCs regulate hepatic immune homeostasis, inflammation, and acute injury.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Medicine, General & Internal
Joanna Maria Lotowska, Maria Elzbieta Sobaniec-Lotowska, Anna Bobrus-Chociej, Piotr Sobaniec
Summary: The aim of the study was to evaluate the interaction between hepatic stellate cells (HSCs) and adjacent nonparenchymal cells (NPCs) in pediatric autoimmune hepatitis (AIH). The study found that T-HSCs, including iHSCs and pHSCs, play a key role in liver fibrogenesis in AIH.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Engineering, Biomedical
Li Xiang, Xin Wang, Qiangqiang Jiao, Yaru Shao, Rui Luo, Jie Zhang, Xiaotong Zheng, Shaobing Zhou, Yuping Chen
Summary: In this study, vitamin A-decorated PEG-PCL polymeric micelles were developed to encapsulate camptothecin and target hepatic stellate cells (HSCs). The micelles effectively inhibited glycolysis in HSCs and suppressed the progression of liver fibrosis.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Yang You, Chongqing Gao, Junru Wu, Hengdong Qu, Yang Xiao, Ziwei Kang, Jinying Li, Jian Hong
Summary: ARK5 plays a critical role in liver fibrosis by maintaining the continuous transduction of the TGF-beta signaling pathway in HSCs and inducing epithelial-mesenchymal transition and inflammatory factor secretion in hepatocytes, thereby promoting liver fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Li Ma, Junping Liu, Erhui Xiao, Huibin Ning, Kuan Li, Jia Shang, Yi Kang
Summary: miR-15b/16 exhibit anti-fibrotic activity through regulation of the Smad2/3 pathway by inhibiting LOXL1 expression.
Article
Gastroenterology & Hepatology
Qinghui Zhang, Rongrong Jia, Minjie Chen, Jianjun Wang, Feng Huang, Min Shi, Huiming Sheng, Ling Xu
Summary: The active form of vitamin D3, 1,25-OH2 Vitamin D3, is responsible for anti-fibrosis and improves liver function. GSK126, an Ezh2 inhibitor, plays a synergistic role in this process. Co-culture experiments showed that hepatocyte autophagy increases after activation of hepatic stellate cells, which can be reduced by supplementation with 1,25-OH2 Vitamin D3 or combined GSK126. Further research revealed that 1,25-OH2 Vitamin D3 promotes H3K27 methylation of the DKK1 promoter through VDR/Ezh2, weakening the inhibitory signal of hepatic stellate cells.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Cell Biology
Wenjun Zhang, Simon J. Conway, Ying Liu, Paige Snider, Hanying Chen, Hongyu Gao, Yunlong Liu, Kadir Isidan, Kevin J. Lopez, Gonzalo Campana, Ping Li, Burcin Ekser, Heather Francis, Weinian Shou, Chandrashekhar Kubal
Summary: This study identified multiple subpopulations of HSCs and characterized their unique roles and characteristics during liver injury, including differentiating into myofibroblasts and potential involvement in liver regeneration, immune reaction, and vascular remodeling. The scRNA-seq data provided insight into the dynamic transition from HSCs to myofibroblasts in response to liver injury, highlighting the heterogeneity and functional diversity of HSCs. The findings also suggest similarities between the heterogeneity of HSCs in injured mouse livers and cirrhotic human livers.
Article
Biochemistry & Molecular Biology
Lin Leilei, Sun Xue, Li Yan, Luo Yuyuan, Wang Ying, Qiu Wenke, Yu Xuesong, Li Ming
Summary: Recent studies have shown that TGF-beta 1 secreted from hepatocytes exposed to PM2.5 may lead to activation of hepatic stellate cells, potentially contributing to liver fibrosis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Zhen-Yu Cui, Ge Wang, Jing Zhang, Jian Song, Yu-Chen Jiang, Jia-Yi Dou, Li-Hua Lian, Ji-Xing Nan, Yan-Ling Wu
Summary: Parthenolide (PNL) was found to decrease the expression of fibrosis markers, reduce the release of inflammatory cytokines, and induce apoptosis in activated hepatic stellate cells (HSCs) by regulating the crosstalk of toll-like receptor 4 (TLR4) and signal transducer and activator of transcription 3 (STAT3).
PHYTOTHERAPY RESEARCH
(2021)
Article
Gastroenterology & Hepatology
Jing Yang, Xujiao Tang, Zhu Liang, Mingzhu Chen, Lixin Sun
Summary: This study investigated the effects of bile acids on the activation of hepatic stellate cells (HSCs) and identified the S1PR2/p38 MAPK/YAP signaling pathway as a key regulator of HSC activation. These findings have therapeutic implications for targeting cholestatic liver fibrosis.
CLINICAL AND MOLECULAR HEPATOLOGY
(2023)
Article
Cell Biology
Brandon T. Wesley, Alexander D. B. Ross, Daniele Muraro, Zhichao Miao, Sarah Saxton, Rute A. Tomaz, Carola M. Morell, Katherine Ridley, Ekaterini D. Zacharis, Sandra Petrus-Reurer, Judith Kraiczy, Krishnaa T. Mahbubani, Stephanie Brown, Jose Garcia-Bernardo, Clara Alsinet, Daniel Gaffney, Dave Horsfall, Olivia C. Tysoe, Rachel A. Botting, Emily Stephenson, Dorin-Mirel Popescu, Sonya MacParland, Gary Bader, Ian D. McGilvray, Daniel Ortmann, Fotios Sampaziotis, Kourosh Saeb-Parsy, Muzlifah Haniffa, Kelly R. Stevens, Matthias Zilbauer, Sarah A. Teichmann, Ludovic Vallier
Summary: In this study, the developmental trajectories of human fetal liver cell types were described at single-cell resolution, and bipotential hepatoblast organoids were generated. This provides a platform for investigating human liver development and producing cell types for clinical applications.
NATURE CELL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Ren Guo, Xiaohui Jia, Zhenbin Ding, Gang Wang, Mengmeng Jiang, Bing Li, Shanshan Chen, Bingqing Xia, Qing Zhang, Jian Liu, Ruting Zheng, Zhaobing Gao, Xin Xie
Summary: MLKL plays an important role in liver damage and fibrosis, and targeting MLKL could be an effective way to treat liver fibrosis.
Article
Gastroenterology & Hepatology
Jacey J. Liu, Bing Xin, Li Du, Lydia Chen, Yanyan Long, Gen-Sheng Feng
Summary: This study reveals the paradoxical functions of Shp2 in hepatocarcinogenesis and highlights the potential of Shp2 inhibitors as a therapeutic target for primary and metastasized liver cancer. The catalytic activity of Shp2 is essential for oncogenic signal transmission, but inhibition of Shp2 can suppress liver cancer driven by receptor tyrosine kinases and enhance antitumor immunity.
Article
Medicine, General & Internal
Ali Eqbal, Alicia Martin, James D. Doecke, Desmond Patrick
Summary: This study evaluated the short-term efficacy and safety of low-dose therapeutic drug-monitored (TDM) thioguanine in patients with inflammatory bowel disease. The results showed that thioguanine was well tolerated in 63% of patients, with a clinical response rate of 62% and a high maintenance of remission rate at 76%.
INTERNAL MEDICINE JOURNAL
(2023)
Article
Clinical Neurology
Pratishtha Chatterjee, Steve Pedrini, James D. Doecke, Rohith Thota, Victor L. Villemagne, Vincent Dore, Abhay K. Singh, Penghao Wang, Stephanie Rainey-Smith, Christopher Fowler, Kevin Taddei, Hamid R. Sohrabi, Mark P. Molloy, David Ames, Paul Maruff, Christopher C. Rowe, Colin L. Masters, Ralph N. Martins
Summary: This study investigated the changes in several blood biomarkers across the AD continuum and their associations with cognitive decline and brain Aβ-PET load. The results showed that plasma Aβ1-42/Aβ1-40 ratio decreased, p-tau181 and GFAP increased in predicting the β-amyloid positive/negative status across the AD continuum.
ALZHEIMERS & DEMENTIA
(2023)
Article
Gastroenterology & Hepatology
Sally Mortlock, Anton Lord, Grant Montgomery, Martha Zakrzewski, Lisa A. Simms, Krupa Krishnaprasad, Katherine Hanigan, James D. Doecke, Alissa Walsh, Ian C. Lawrance, Peter A. Bampton, Jane M. Andrews, Gillian Mahy, Susan J. Connor, Miles P. Sparrow, Sally Bell, Timothy H. Florin, Jakob Begun, Richard B. Gearry, Graham L. Radford-Smith
Summary: This study identified genetic loci associated with medically refractory ulcerative colitis (UC) through a genome wide association analysis. The findings suggest different genetic risk factors for medically refractory UC compared to non-medically refractory UC. Further research may uncover additional loci related to disease severity.
JOURNAL OF CROHNS & COLITIS
(2023)
Article
Clinical Neurology
Xin Huang, Yihan Li, Christopher Fowler, James D. Doecke, Yen Ying Lim, Candace Drysdale, Vicky Zhang, Keunha Park, Brett Trounson, Kelly Pertile, Rebecca Rumble, John W. Pickering, Robert A. Rissman, Floyd Sarsoza, Sara Abdel-Latif, Yong Lin, Vincent Dore, Victor Villemagne, Christopher C. Rowe, Jurgen Fripp, Ralph Martins, James S. Wiley, Paul Maruff, Jacobo E. Mintzer, Colin L. Masters, Ben J. Gu
Summary: This study examined leukocyte antigens in sporadic Alzheimer's disease (AD) and identified differentially expressed markers. A proposed panel of four leukocyte markers showed high predictive accuracy for PET A beta status. These findings were validated in independent cohorts, demonstrating the utility of leukocyte-based biomarkers for AD screening and diagnosis.
ALZHEIMERS & DEMENTIA
(2023)
Article
Biochemical Research Methods
Licai Huang, James P. Long, Ehsan Irajizad, James D. Doecke, Kim-Anh Do, Min Jin Ha
Summary: This study introduces a mediation analysis framework for multilevel molecular tumor profiling, which can handle various types of outcome variables and is suitable for high-throughput molecular profiling data. By analyzing kidney renal clear cell carcinoma proteogenomic data, genes that are mediated by proteins and the underlying mechanisms for various survival outcomes are identified, capturing disease-specific clinical characteristics in both short and long-term.
Article
Neurosciences
Charisse N. Winston, Oliver Langford, Natalie Levin, Rema Raman, Kevin Yarasheski, Tim West, Sara Abdel-Latif, Michael Donohue, Akinori Nakamura, Kenji Toba, Colin L. Masters, James Doecke, Reisa A. Sperling, Paul S. Aisen, Robert A. Rissman
Summary: The study aimed to determine whether a blood-based screening test can accurately identify patients' amyloid burden and improve screening efficiency. The results showed that plasma A beta (42)/A beta (40) is the best biomarker for predicting amyloid accumulation in the brain, and blood samples processed within 2 hours after collection had better predictive performance.
JOURNAL OF ALZHEIMERS DISEASE
(2023)
Article
Agronomy
Andressa Leal Generoso, Virginia Silva Carvalho, Roberta Aparecida Sales, Naiara Lopes Brito, Alexandre Pio Viana, Telma Nair Santana Pereira
Summary: The conservation of Passiflora L. germplasm through seed banks and field collections is difficult due to dormant seeds, seeds with low viability, and high-cost field collections. In vitro conservation can serve as a complementary alternative. This study investigated the survival of nodal segments of Passiflora edulis Sims 'UENF Rio Dourado' over 180 days by modifying the mineral salt and sucrose concentrations and changing the incubation conditions. The results showed that the combination of 25% MSM mineral salts, 10 g L-1 sucrose, and an average temperature of 20 degrees C allowed for slow growth and did not compromise survival, regeneration, and acclimatization of the passion fruit segments.
ACTA SCIENTIARUM-AGRONOMY
(2023)
Article
Cardiac & Cardiovascular Systems
Wei Wu, Qun Lu, Shan Ma, Jin-Chan Du, Kevin Huynh, Thy Duong, Zhang-Da Pang, Daniel Donner, Peter J. Meikle, Xiu-Ling Deng, Xiao-Jun Du
Summary: Using an isoproterenol-induced mouse model of Takotsubo syndrome (TTS), we investigated the role of the O-adrenoceptors (OAR)-Hippo signaling pathway in mediating mitochondrial dysfunction. Our findings demonstrate that activation of OAR stimulates the Hippo pathway, which leads to mitochondrial dysfunction characterized by energy insufficiency and increased reactive oxygen species.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Endocrinology & Metabolism
Guillaume Treiber, Alice Guilleux, Kevin Huynh, Oriane Bonfanti, Ania Flaus-Furmaniuk, David Couret, Natalie Mellet, Celine Bernard, Nathalie Le-Moullec, Berenice Doray, Isabelle Jeru, Jean-Christophe Maiza, Bhoopendrasing Domun, Muriel Cogne, Olivier Meilhac, Corinne Vigouroux, Peter J. Meikle, Estelle Nobecourt
Summary: The study investigated glucose tolerance, insulin response, and metabolic markers in FPLD2 patients, revealing a high prevalence of diabetes and prediabetes. Early diagnosis and treatment are necessary.
DIABETES & METABOLISM
(2023)
Review
Biology
Aaron W. Jurrjens, Marcus M. Seldin, Corey Giles, Peter J. Meikle, Brian G. Drew, Anna C. Calkin
Summary: Cardiometabolic diseases result from genetic, environmental, and lifestyle factors and identifying those at high risk is challenging. Systems genetics, using population-based approaches, can elucidate the genetic and environmental causes of these diseases. Mouse genetic reference panels provide a complementary approach to human genome-wide association studies, allowing for controlled investigation of genetic and phenotypic variation. Integrating multi-omics data from human and mouse populations can advance our understanding of cardiometabolic diseases.
Article
Clinical Neurology
Matteo Senesi, Victoria Lewis, Shiji Varghese, Christiane Stehmann, Amelia McGlade, James D. Doecke, Laura Ellett, Shannon Sarros, Christopher J. Fowler, Colin L. Masters, Qiao-Xin Li, Steven J. Collins
Summary: The most frequently used biomarkers for pre-mortem clinical diagnosis of sporadic Creutzfeldt-Jakob disease (sCJD) include concentrations of the 14-3-3 and total tau (T-tau) proteins, as well as the application of protein amplification techniques. Combining the results of these three cerebrospinal fluid biomarkers can increase the sensitivity and accuracy in diagnosing sCJD before death, providing the best chance for early detection.
FRONTIERS IN NEUROLOGY
(2023)
Article
Nutrition & Dietetics
Paige F. van der Pligt, Konsita Kuswara, Sarah A. Mcnaughton, Gavin Abbott, Sheikh Mohammed Shariful Islam, Kevin Huynh, Peter J. Meikle, Aya Mousa, Stacey J. Ellery
Summary: This study aimed to assess the relationship between early pregnancy maternal diet quality and maternal plasma lipids and indicators of cardiometabolic health. The results showed that maternal diet quality was inversely associated with multiple plasma triglycerides. This study provides novel insights into the relationship between diet quality, lipid biomarkers, and cardiometabolic health during pregnancy.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Gastroenterology & Hepatology
Ying Li, Bhagirath Chaurasia, M. Mahidur Rahman, Vincent Kaddai, J. Alan Maschek, Jordan A. Berg, Joseph L. Wilkerson, Ziad S. Mahmassani, James Cox, Peng Wei, Peter J. Meikle, Donald Atkinson, Liping Wang, Annelise M. Poss, Mary C. Playdon, Trevor S. Tippetts, Esraa M. Mousa, Kesara Nittayaboon, Pon Velayutham Anandh Babu, Micah J. Drummond, Hans Clevers, James A. Shayman, Yoshio Hirabayashi, William L. Holland, Jared Rutter, Bruce A. Edgar, Scott A. Summers
Summary: This study found that ceramides, as sphingolipid compounds, are associated with alimentary tract cancers. They function as signals of nutritional excess and can alter stem cell behaviors to influence cancer risk. The study also discovered that sphingolipid-producing enzymes are up-regulated in intestinal adenomas, leading to increased proliferation of intestinal progenitors through the stimulation of peroxisome-proliferator activated receptor-alpha and induction of fatty acid binding protein-1.
Article
Cell Biology
Hyo Lee, Aimee J. Aylward, Richard Pearse II, Alexandra M. Lish, Yi-Chen Hsieh, Zachary M. Augur, Courtney R. Benoit, Vicky Chou, Allison Knupp, Cheryl Pan, Srilakshmi Goberdhan, Duc M. Duong, Nicholas T. Seyfried, David A. Bennett, Mariko F. Taga, Kevin Huynh, Matthias Arnold, Peter J. Meikle, Philip L. De Jager, Vilas Menon, Jessica E. Young, Tracy L. Young-Pearse
Summary: SORL1 plays an important role in the pathogenesis of Alzheimer's disease. Loss of SORL1 affects neurons and astrocytes the most and leads to a reduction in apolipoprotein E (APOE) and clusterin (CLU), altered lipid profiles, and tau phenotypes in neurons. The study also identifies a link between SORL1, APOE, and CLU levels in neurons and implicates transforming growth factor β/SMAD signaling in SORL1 function.
Article
Multidisciplinary Sciences
Ebru Boslem, Saskia Reibe, Rodrigo Carlessi, Benoit Smeuninx, Surafel Tegegne, Casey L. Egan, Emma Mclennan, Lauren V. Terry, Max Nobis, Andre Mu, Cameron Nowell, Neil Horadagoda, Natalie A. Mellett, Paul Timpson, Matthew Jones, Elena Denisenko, Alistair R. R. Forrest, Janina E. E. Tirnitz-Parker, Peter J. Meikle, Stefan Rose-John, Michael Karin, Mark A. Febbraio
Summary: The incidence of hepatocellular carcinoma (HCC) is increasing due to obesity-related nonalcoholic steatohepatitis (NASH). In a mouse model mimicking NASH-driven HCC, activation of endoplasmic reticulum stress and inflammation was observed in hepatocytes. Treatment with an ER stress inhibitor and an anti-inflammatory drug reversed NASH and reduced NASH-driven HCC.