Article
Biochemistry & Molecular Biology
Qian Liu, Ruotong Tian, Panpan Yu, Minfeng Shu
Summary: The study identified that increasing cAMP levels can induce lineage-specific differentiation of glioma cells, with miR-221/222 suppression contributing to neuronal differentiation. The findings highlight the crucial role of the miR-221/222 cluster as an epigenetic brake during glioma differentiation.
Article
Cardiac & Cardiovascular Systems
Jie Su, Pierre Coleman, Angeliki Ntorla, Rhys Anderson, Michael J. Shattock, Joseph R. Burgoyne
Summary: This study demonstrates that the cytosolic DNA sensor cGAS can activate PKGI directly through the generation of cGAMP, showing a link between inflammation and blood pressure regulation.
Article
Biochemistry & Molecular Biology
Feifei Wang, Gang Yu, Qing Yang, Xiao Yi, Linglin Fu, Yanbo Wang
Summary: In this study, a homogeneous polysaccharide was isolated from edible red seaweed and used as a biopolymer matrix to form composite films with small biological molecules. The addition of these molecules improved the barrier properties, wettability, mechanical strength, and antimicrobial activity of the films. The film composed of cAMP and the polysaccharide showed the best characteristics and was applied in fish packaging, extending the shelf life.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Immunology
Eveline Hofmann, Bernard Surial, Noemie Boillat-Blanco, Huldrych F. Guenthard, Marcel Stoeckle, Enos Bernasconi, Patrick Schmid, Alexandra Calmy, Franziska Suter-Riniker, Andri Rauch, Gilles Wandeler, Charles Beguelin
Summary: In the Swiss HIV Cohort Study, researchers found that 27% of HIV-suppressed individuals with chronic hepatitis B virus (HBV) infection had HBV replication after two years on tenofovir. However, 77% of them were able to achieve suppression thereafter. Self-reported adherence to therapy and HBV viral load at tenofovir initiation were identified as predictors of persistent replication.
CLINICAL INFECTIOUS DISEASES
(2023)
Review
Cell Biology
Zhilei Wang, Nian Chen, Zhiyong Li, Guang Xu, Xiaoyan Zhan, Jianyuan Tang, Xiaohe Xiao, Zhaofang Bai
Summary: Inflammation is a protective response regulated by the host, with cGAS-STING pathway playing a crucial role in immune defense, viral infections, fatty liver, and cancer metastasis. The potential impact of this pathway in liver diseases has attracted widespread attention, with pharmacological agonists and antagonists offering new therapeutic possibilities. Understanding the mechanisms of cGAS-STING pathway may lead to the development of effective treatments for liver diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Aritra Bhadra, April K. Scruggs, Silas J. Leavesley, Naga Annamdevula, April H. George, Andrea L. Britain, Christopher M. Francis, Jennifer M. Knighten, Thomas C. Rich, Natalie N. Bauer
Summary: Second messenger signaling is essential for cellular processes. Our study demonstrates that cAMP-enriched EVs can induce second messenger signaling pathways, revealing the significance of EVs as a novel intercellular mechanism for cAMP signaling.
CELLULAR SIGNALLING
(2022)
Article
Microbiology
Ruizhen Li, Wenli Liu, Xiangrui Yin, Fangfang Zheng, Zhenyu Wang, Xingchen Wu, Xiaohua Zhang, Qian Du, Yong Huang, Dewen Tong
Summary: This study found that Brucella outer membrane protein Omp25 could suppress the production of IFN-beta and its downstream IFN-stimulated genes induced by various DNA viruses or IFN-stimulatory DNA. Omp25 promoted the degradation of cyclic GMP-AMP synthase (cGAS) via the ubiquitin-proteasome-dependent pathway, leading to the inhibition of IFN-beta induction in various mammalian monocyte/macrophages.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Cell Biology
Lufei Wang, Lichi Han, Peng Xue, Xiangxiang Hu, Sing-Wai Wong, Meng Deng, Henry C. Tseng, Bo-Wen Huang, Ching-Chang Ko
Summary: The study found that dopamine inhibits osteoclast differentiation through the D2R/cAMP/PKA/CREB pathway. This reveals a potential mechanism for how the nervous system impacts bone remodeling and suggests future research directions for understanding bone development, repair, aging, and degenerative diseases.
CELLULAR SIGNALLING
(2021)
Article
Biochemical Research Methods
Aiguo Xia, Mingjie Qian, Congcong Wang, Yajia Huang, Zhi Liu, Lei Ni, Fan Jin
Summary: Researchers have developed an engineered Pseudomonas aeruginosa strain with light-dependent intracellular cAMP levels by introducing a photoactivated adenylate cyclase gene. Through blue light illumination, the engineered strain showed increased expression of cAMP responsive promoter and twitching activity, making it suitable for applications in controlling bacterial host infection. Additionally, directional twitching motility of the colonies was achieved through localized light illumination, which will aid in studying contact-dependent interactions between microbial species.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biochemical Research Methods
Guido Domingo, Milena Marsoni, Luca Chiodaroli, Stefania Fortunato, Marcella Bracale, Maria Concetta De Pinto, Chris Gehring, Candida Vannini
Summary: 3',5'-cyclic adenosine monophosphate (cAMP) is recognized as a crucial signaling molecule in plants, and its role in cellular processes such as hormone response and environmental stimuli has been studied. A phosphoproteomic analysis of tobacco BY-2 cells with modulated cAMP levels revealed the significant impact of cAMP on plant kinome, mRNA processing, and cellular programming. The findings suggest the importance of unperturbed cellular cAMP levels for plant cellular homeostasis and signaling.
Article
Microbiology
Chun-yang Gan, Jing Cui, Wen-lu Zhang, Yu-wei Wang, Ai-long Huang, Jie-li Hu
Summary: Recombinant DNA technology is used to engineer exogenous genes into the HBV genome, with potential implications for disrupting the formation of HBV's relaxed circular DNA through the induction of adverse secondary structures. This study provides valuable insights into the construction and optimization of recombinant HBV, highlighting the potential role of DNA sequence in selective pressure during HBV evolution.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Immunology
Hongjuan You, Lihong Ma, Xing Wang, Fulong Zhang, Yiran Han, Jiaqi Yao, Xiucheng Pan, Kuiyang Zheng, Fanyun Kong, Renxian Tang
Summary: DEAD/H-box helicases are essential proteins that regulate RNA metabolism and innate immune responses. They have been implicated in the development of various diseases, particularly those related to HBV infection. Recent studies have shown that DEAD/H-box helicases interact with HBV, modulating viral replication and innate signaling pathways. Targeting these helicases may be a promising treatment strategy for HBV infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Virology
Liudi Tang, Ping An, Qiong Zhao, Cheryl A. A. Winkler, Jinhong Chang, Ju-Tao Guo
Summary: Elimination or functional inactivation of cccDNA minichromosomes is crucial for curing hepatitis B virus (HBV) infection, but the lack of knowledge on the molecular mechanisms hampers the development of antiviral drugs. The study found that a small molecule inhibitor, B02, enhances cccDNA synthesis through a heat shock protein-mediated cellular response.
JOURNAL OF VIROLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Shang-Hung Chang, Yi-Hsin Chan, Wei-Jan Chen, Gwo-Jyh Chang, Jia-Lin Lee, Yung-Hsin Yeh
Summary: The study investigated the potential role of CD44 and its related signaling in tachypacing-induced downregulation of LTCC, showing that CREB/CD44 signaling contributes to the suppression of LTCC, providing valuable insights into atrial remodeling and AF pathogenesis.
Article
Virology
Richeng Mao, Minhui Dong, Zhongliang Shen, Hu Zhang, Yuanjie Liu, Dawei Cai, Bidisha Mitra, Jiming Zhang, Haitao Guo
Summary: DDX17 is a member of the DEAD-box helicase family proteins that inhibits HBV replication through reducing cytoplasmic encapsidated pgRNA levels in a helicase-dependent manner, competing with HBV polymerase to bind to pgRNA at the 5' epsilon motif.
JOURNAL OF VIROLOGY
(2021)