Review
Medicine, General & Internal
Vaneet Jearth, Mitali Madhumita Rath, Abhirup Chatterjee, Aditya Kale, Manas Kumar Panigrahi
Summary: Helicobacter pylori is a common chronic bacterial infection affecting half of the world's population. It is classified as a Class I carcinogen and is linked to the majority of stomach cancer cases worldwide. Antibiotic resistance in H. pylori is a major public health concern and a leading cause of eradication failure. This review provides an overview of H. pylori antibiotic resistance patterns, mechanisms, and clinical implications, as well as a discussion on current testing methods and evidence-based approaches to treatment.
Review
Infectious Diseases
Paula Rojas Garcia, Simon van der Pol, Antoinette D. I. van Asselt, Maarten Postma, Roberto Rodriguez-Ibeas, Carmelo A. Juarez-Castello, Marino Gonzalez, Fernando Antonanzas
Summary: The study compared the costs and clinical effectiveness of diagnostic strategies for H. pylori infections and found testing to be the most cost-effective strategy in most cases. Some studies considered antimicrobial resistance (AMR) and found that incorporating AMR into the model may alter the efficiency of the testing strategy.
Article
Chemistry, Multidisciplinary
Yanqi Qiao, Yan Ma, Yue Tong, Wenting Liu, Shuo Wang, Ying Zheng, Changjun Men, Jie Yu, Jie Pan, Dong Wan, Yongmei Yin, Xiujie Zhao, Rimo Xi, Meng Meng
Summary: A new near-infrared photosensitizer has been developed for effective phototherapy against H. pylori infections and resistant strains, showing synergistic photothermal therapy/photodynamic therapy effects. RNA-seq analysis revealed the potential mechanism of efficient H. pylori phototherapy.
Article
Immunology
Xiaoqiong Tang, Yalin Shen, Xiaona Song, Mohammed Benghezal, Barry J. Marshall, Hong Tang, Hong Li
Summary: This study assessed the applicability of broth microdilution as a routine susceptibility testing method for Helicobacter pylori. The results showed a good correlation between the MICs obtained by the broth microdilution method and the agar dilution method, indicating that the broth microdilution method is suitable for susceptibility testing of clinical H. pylori isolates.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Asif Sukri, Alfizah Hanafiah, Hamidah Yusoff, Nur Atiqah Shamsul Nizam, Zarith Nameyrra, Zhiqin Wong, Raja Affendi Raja Ali
Summary: This study aimed to estimate the prevalence of secondary antibiotic resistance of Helicobacter pylori in urban multicultural areas in Malaysia. The results showed high resistance rates of H. pylori to multiple antibiotics, particularly dual resistance to clarithromycin and metronidazole. Additionally, amoxicillin-resistant H. pylori strains and their resistance mechanisms were reported for the first time in Malaysian patients.
Article
Chemistry, Multidisciplinary
Yen-Yu Tseng, Jyh-Ming Liou, Wei-Chieh Cheng, Jing-Ting Hsu, Tsui-Ling Hsu, Ming-Shiang Wu, Chi-Huey Wong
Summary: Combining moenomycin A with clarithromycin or metronidazole shows a synergistic effect in eradicating multidrug-resistant Helicobacter pylori, with a near 95% success rate. Moenomycin A-non-susceptible strains of H. pylori with transglycosylase deletion exhibit slower growth and impaired biofilm formation compared to the parental strain.
FRONTIERS IN CHEMISTRY
(2022)
Review
Microbiology
Raluca Ioana Dascalu, Alexandra Bolocan, Dan Nicolae Paduaru, Alexandru Constantinescu, Magda Mihaela Mitache, Anca Daniela Stoica, Octavian Andronic
Summary: Helicobacter pylori (Hp) is a common pathogen causing chronic bacterial infections and gastrointestinal diseases. The prevalence of Hp infection varies worldwide and is related to socio-economic status, especially during childhood. The increasing antibiotic resistance, poor patient compliance, and bacterial-related factors contribute to the challenge in successfully eradicating Hp. New eradication regimens and strain susceptibility testing are important for effective treatment.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Pediatrics
Capan Konca, Mehmet Tekin, Mehmet Geyik
Summary: The study investigated the antimicrobial resistance patterns of multidrug-resistant Acinetobacter baumannii in pediatric intensive care unit patients. Results showed that many previously effective drugs have lost their effectiveness against MDRAB, with colistin remaining as the only effective drug. New drugs and treatment protocols are urgently needed to combat MDRAB.
INDIAN JOURNAL OF PEDIATRICS
(2021)
Article
Genetics & Heredity
Shakti Kumar, Tanshi Mehrotra, Daizee Talukdar, Jyoti Verma, Bipul Chandra Karmakar, Sangita Paul, Sujit Chaudhuri, Agila Kumari Pragasam, Susmita Bakshi, Shashi Kumari, Meenal Chawla, Ayushi Purohit, Sonali Porey Karmakar, Ankur Mutreja, Sayantan Banerjee, Animesh Ray, Thandavarayan Ramamurthy, Asish K. Mukhopadyay, Bhabatosh Das
Summary: This study analyzed the whole genome sequences of 143 Helicobacter pylori strains isolated from different regions of India and found distinct genomic signatures and antimicrobial resistance phenotypes. The strains from Eastern India showed higher resistance to metronidazole and clarithromycin, while lower resistance to amoxicillin and furazolidone. Moreover, more antimicrobial resistance genes were observed in the Eastern Indian strains compared to the Southern Indian strains. The study also revealed phylogenetic differences among the H. pylori strains based on their geographical locations, with conditionally beneficial functions, including antibiotic resistance phenotypes, being linked to faster evolution rates in the Indian isolates.
Article
Oncology
Zishao Zhong, Zhenyu Zhang, Jing Wang, Yunlian Hu, Yang Mi, Bangshun He, Yushu Zhang, Ximei Zhang, Xingzhou Xia, Huang Huang, Yuexing Lai, Min Lin, Chengxia Su, Zhiyi Zhang, Zhengqi Wu, Linzhi Lu, Beiping Zhang, Suiping Huang, Cailing Zhong, Xiaoming Zeng, Yun Peng, Guangxia Chen, Haihan Zhang, Guangqing Zhou, Shiyu Liu, Changqing Yang, Lijuan Yan, Aojun Chen, Guiying Zhang, Ping Xu, Shukui Wang, Pengyuan Zheng, Shuchang Xu, Hengjun Gao
Summary: Helicobacter pylori antibiotic resistance is a serious concern in China, where personalized therapies based on genotype testing could partially replace traditional antibiotic susceptibility testing. Continuous monitoring and personalized treatments remain necessary for effective management of H. pylori infections in China.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Review
Gastroenterology & Hepatology
Hong Li, Yalin Shen, Xiaona Song, Xiaoqiong Tang, Renwei Hu, Barry James Marshall, Hong Tang, Mohammed Benghezal
Summary: This review calls for international consensus on standardization and harmonization of susceptibility testing for Helicobacter pylori. Standard phenotypic testing methods and molecular testing methods each have their own advantages and disadvantages. Efforts are needed to optimize culture methods and develop high throughput and automated testing for H. pylori to make susceptibility testing a routine clinical practice.
Article
Microbiology
Yuhang Wang, Junfeng Su, Ziyan Zhou, Jie Yang, Wenjuan Liu, Yafen Zhang, Pengyu Zhang, Tingting Guo, Guocai Li
Summary: As multidrug-resistant pathogens emerge and spread rapidly, novel antibiotics are urgently needed. Traditional Chinese medicine, such as baicalein, can be used as an adjuvant to enhance the efficacy of antibiotics. Baicalein was found to potentiate doxycycline against multidrug-resistant Gram-negative pathogens by disrupting the bacterial membrane and enhancing antibiotic entry. It can also increase the production of reactive oxygen species and inhibit multidrug efflux pumps and biofilm formation to potentiate antibiotic efficacy.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Salika M. Shakir, Joshua Otiso, George Keller, Hillary Van Heule, Lucas J. Osborn, Nicolynn Cole, Audrey N. Schuetz, Sandra S. Richter, Marc Roger Couturier
Summary: This article describes the importance of antimicrobial susceptibility testing (AST) for Helicobacter pylori and the performance of the Etest method in comparison to the agar dilution (AD) reference method. The study found that the optimized Etest method showed acceptable performance for H. pylori AST.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Multidisciplinary
William Ndugire, Dang Truong, N. G. Hasitha Raviranga, Jingzhe Lao, Olof Ramstrom, Mingdi Yan
Summary: The addition of thiourea (TU) enhances the broad-spectrum antimicrobial activity of D-maltose-capped gold nanoclusters (AuNC-Mal). AuNC-Mal/TU exhibits effective inhibition against multidrug-resistant Pseudomonas aeruginosa with low cytotoxicity. The antimicrobial activity is attributed to the generation of [Au(TU)(2)](+) and smaller AuNCs, increased accumulation of Au in bacteria, and inhibition of thioredoxin reductase.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Microbiology
Chia-Ru Chung, Zhuo Wang, Jing-Mei Weng, Hsin-Yao Wang, Li-Ching Wu, Yi-Ju Tseng, Chun-Hsien Chen, Jang-Jih Lu, Jorng-Tzong Horng, Tzong-Yi Lee
Summary: This study developed a web tool called MDRSA for the rapid identification of oxacillin-, clindamycin-, and erythromycin-resistant Staphylococcus aureus. The kernel density estimation (KDE) was used to deal with the peak shifting problem, and machine learning methods were employed to construct classifiers to identify antibiotic resistance. The promising results from internal and external validation suggest the potential application of these prediction models in the real world.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Oncology
Maria Joao Carvalho, Mafalda Laranjo, Ana Margarida Abrantes, Joao Casalta-Lopes, Daniela Sarmento-Santos, Tania Costa, Beatriz Serambeque, Nuno Almeida, Telmo Goncalves, Catarina Mamede, Joao Encarnacao, Rui Oliveira, Artur Paiva, Rui de Carvalho, Filomena Botelho, Carlos Oliveira
PATHOLOGY & ONCOLOGY RESEARCH
(2019)
Article
Infectious Diseases
Bruno Marques, Maria Manuel Donato, Olga Cardoso, Cristina Luxo, Antonio Martinho, Nuno Almeida
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2019)
Article
Microbiology
Andreia F. Santos, Catia P. Santos, Ana M. Matos, Olga Cardoso, Margarida J. Quina
Article
Infectious Diseases
Olga Cardoso, Sofia Osorio, Fernando Ramos, Maria Manuel Donato
Summary: This study aimed to determine the susceptibility profiles of Escherichia coli in piglets' intestinal microbiota from different farms in Portugal, with a focus on antimicrobial resistance and the detection of ESBLs and pAmpC. The study found a high rate of MDR E. coli isolates in Portuguese piglets, with 65% of the isolates carrying pAmpC and/or ESBLs genes, with the most common combination being ESBL (TEM) and pAmpC (CMY-2).
MICROBIAL DRUG RESISTANCE
(2021)
Article
Environmental Sciences
Andreia F. Santos, Telma E. Vaz, Daniela Lopes, Olga Cardoso, Margarida J. Quina
Summary: This study proposes a new approach for converting sewage sludge and lime mud into value-added products within the circular economy perspective. The addition of calcined lime mud effectively eliminates Escherichia coli contamination in most cases and improves the drying process. Understanding the beneficial effects of adding calcined lime mud can potentially lead to the production of a safer organic soil improver.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Gastroenterology & Hepatology
Catarina Correia, Nuno Almeida, Carina Leal, Diogo Branquinho, Alexandra Fernandes, Elisa Gravito-Soares, Carlos Calhau, Isabel Bastos, Helena Vasconcelos, Pedro Figueiredo
Summary: In Portugal, a single-capsule bismuth-based quadruple therapy is an excellent option as a second-line or rescue therapy, with acceptable compliance and side effects.
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Gastroenterology & Hepatology
Catarina J. A. Correia, Nuno Almeida, Lagchar Barreto, Pedro N. Figueiredo
Summary: This case presents a middle-aged woman who accidentally swallowed a portion of a nasopharyngeal swab during a diagnostic test for SARS-CoV-2. Prompt upper gastrointestinal endoscopy was performed to remove the swab and prevent complications. This is the second reported case of swab ingestion during SARS-CoV-2 testing to our knowledge.
ADVANCES IN DIGESTIVE MEDICINE
(2023)
Article
Gastroenterology & Hepatology
Nuno Miguel de Almeida, Alexandra Fernandes, Jose Manuel Romaozinho, Paulo Freire, Maria M. Donato, Olga Cardoso, Cristina Luxo, Maria Augusta Cipriano, Carol Marinho, Carlos Calhau, Pedro Figueiredo
Summary: The study investigated whether NOD2 mutations increased the risk of H. pylori infection and found that these mutations were not associated with increased prevalence of infection or more severe gastric histopathological modifications induced by the bacterium, but could potentially influence clinical manifestations.
ADVANCES IN DIGESTIVE MEDICINE
(2021)
Letter
Medicine, General & Internal
Eduarda Sa-Marta, Nuno Almeida
ACTA MEDICA PORTUGUESA
(2020)
Article
Gastroenterology & Hepatology
Enrique Perez-Cuadrado-Robles, Rolando Pinho, Begona Gonzalez, Susana Mao de Ferro, Cristina Chagas, Pilar Esteban Delgado, Cristina Carretero, Pedro Figueiredo, Bruno Rosa, Javier Garcia Lledo, Oscar Nogales, Ana Ponte, Patricia Andrade, Jose Francisco Juanmartinena-Fernandez, Mileidis San-Juan-Acosta, Sandra Lopes, Cesar Prieto-Frias, Juan Egea-Valenzuela, Noemi Caballero, Eduardo Valdivieso-Cortazar, Helder Cardoso, Consuelo Galvez, Nuno Almeida, Pilar Borque Barrera, Blas Jose Gomez-Rodriguez, Francisco Sanchez Ceballos, Carlos Bernardes, Pedro Alonso, Federico Arguelles-Arias, Miguel Mascarenhas Saraiva, Enrique Perez-Cuadrado-Martinez
REVISTA ESPANOLA DE ENFERMEDADES DIGESTIVAS
(2020)
Article
Gastroenterology & Hepatology
David N. Perdigoto, Dario Gomes, Nuno Almeida, Sofia Mendes, Ana Rita Alves, Ernestina Camacho, Luis Tome
GE PORTUGUESE JOURNAL OF GASTROENTEROLOGY
(2019)
Article
Microbiology
Sonia Goncalves Pereira, Susana Alarico, Igor Tiago, Diogo Reis, Daniela Nunes-Costa, Olga Cardoso, Ana Maranha, Nuno Empadinhas