Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Genetics & Heredity
Jovana Kuveljic, Tamara Djuric, Goran Stankovic, Milica Dekleva, Aleksandra Stankovic, Dragan Alavantic, Maja Zivkovic
Summary: The study did not find a significant association between PHACTR1 variants and MI, but a slightly higher frequency of the rs2876300G allele in MVD. PHACTR1 mRNA was significantly increased in PBMCs of patients six months after MI, and patients carrying the ACG haplotype had increased PHACTR1 mRNA expression. There was no effect of PHACTR1 variants on EDN1 mRNA expression.
Article
Medicine, General & Internal
Zongying Yu, Yang Ling, Qiancheng Xu, Yuhan Cao, Shengxing Tang, Cong Fu
Summary: Blocking A(2B)AR alleviates myocardial damage and improves myocardial systolic function through inhibition of spleen-derived MDSC mobilisation after AMI. Spleen-derived MDSC mobilisation aggravates myocardial inflammatory injury within 24 h of AMI. A(2B)AR promotes spleen-derived MDSC mobilisation within 24 h of AMI. Blocking A(2B)AR improves myocardial systolic function through inhibition of spleen-derived MDSC mobilisation.
ANNALS OF MEDICINE
(2022)
Article
Genetics & Heredity
Jin Zhou, Shaolin He, Boyuan Wang, Wenling Yang, Yuqi Zheng, Shijiu Jiang, Dazhu Li, Jibin Lin
Summary: This study aims to explore the mechanism of circRNA-related ceRNA network in AMI and identify the corresponding immune infiltration characteristics. The results contribute to understanding the mechanism of AMI and providing potential therapeutic targets in the future.
FRONTIERS IN GENETICS
(2022)
Review
Biochemistry & Molecular Biology
Yu Wang, Meiping Wu, Haidong Guo
Summary: Myocardial infarction (MI) is a severe and highly fatal disease. Regenerative approaches for MI have been limited and ineffective due to the loss of cardiomyocytes (CMs) with limited regenerative capacity. Gene therapy using modified mRNA (modRNA) as a delivery vector is a promising approach for promoting myocardial regeneration. This article discusses the optimization of modRNA-based therapy and its effectiveness in treating animal models of MI. The potential benefits of modRNA therapy include promoting CM proliferation and differentiation, inhibiting apoptosis of CMs, and enhancing paracrine effects to promote angiogenesis and inhibit fibrosis in the heart. However, further clinical trials are needed to make modRNA therapy practical and feasible for real-world MI treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Computer Science, Artificial Intelligence
Fernando O. Campos, Aurel Neic, Caroline Mendonca Costa, John Whitaker, Mark O'Neill, Reza Razavi, Christopher A. Rinaldi, DanielScherr, Steven A. Niederer, Gernot Plank, Martin J. Bishop
Summary: Catheter ablation is currently the only curative treatment for scar-related ventricular tachycardias (VTs). However, the long duration, high risk, and low success rates of ablation procedures pose limitations. In this study, researchers developed a novel computational approach called VITA, which can rapidly induce VT and identify ablation targets. VITA was tested on virtual models and demonstrated the ability to detect scar-related VTs and compute ablation targets in a much shorter time compared to traditional simulations.
MEDICAL IMAGE ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Daniel Cesarini, Iacopo Muraca, Martina Berteotti, Anna Maria Gori, Andrea Sorrentino, Alessia Bertelli, Rossella Marcucci, Renato Valenti
Summary: Ticagrelor is a recommended medication for DAPT in ACS patients, but it is associated with increased risk of dyspnea and bradyarrhythmias. The molecular mechanisms of these side effects and their management strategies are not well-defined. This article presents a case report and reviews the available evidence to propose decision-making algorithms for managing these side effects in clinical practice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Carolin Torregroza, Chiara O. Glashoerster, Katharina Feige, Martin Stroethoff, Annika Raupach, Andre Heinen, Markus W. Hollmann, Ragnar Huhn
Summary: The study found that Mannitol achieves cardioprotective effects through activation of the adenosine A1 receptor, rather than the protein kinases Akt and PKG. Akt and PKG are not involved in the preconditioning cardioprotection induced by Mannitol.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Corrado De Marco, Thierry Charron, Guy Rousseau
Summary: Adenosine has the potential benefits in the treatment of acute myocardial infarction by inhibiting the pathophysiological processes leading to reperfusion injury. However, the heterogenous evidence regarding its effects has resulted in limited uptake in clinical practice. Therefore, studying the dose, route, timing, and site of administration of adenosine is crucial for its efficacy.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Wenbo Peng, Danyang Sun, Wei Lu, Shenyi Yin, Buqing Ye, Xiaoqi Wang, Yongan Ren, Zichen Hong, Wenyu Zhu, Pengfei Yu, Jianzhong Jeff Xi, Bo Yao
Summary: A method for simultaneous detection of PD-L1 protein and mRNA in extracellular vesicles (EVs) and their parental cells was developed using qPCR. The expression of exosomal PD-L1 in patient-derived tumor clusters (PTCs) was found to be correlated with tumor types and significantly higher in plasma-derived EVs from tumor patients compared to healthy individuals. This comprehensive detection of PD-L1 at four levels provides a promising tool for predicting the benefits of immunotherapy.
ANALYTICAL CHEMISTRY
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Purvi Parwani, Deepak L. Bhatt
Summary: This editorial discusses a study on non-ST-segment elevation myocardial infarction (NSTEMI) patients, focusing on disparities of care in patients without standard modifiable risk factors. The study, based on a nationwide cohort, provides important insights into this population.
EUROPEAN JOURNAL OF PREVENTIVE CARDIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Biochemistry & Molecular Biology
Zhiwei Chen, Nigel G. Halford, Chenghong Liu
Summary: Real-time quantitative PCR is a technique for measuring the content of a specific nucleic acid sequence in a sample, and it can be divided into absolute and relative quantitative methods. Relative quantification is mainly used for gene expression in functional genomics and transcriptome studies. However, to accurately use this technology, key points such as designing specific primers, selecting appropriate reference genes, and performing scientific calculations and statistics are crucial. This article proposes a workflow for relative quantitative PCR and introduces relevant points to help beginners better understand and utilize this technology.
Article
Cardiac & Cardiovascular Systems
Senlei Xu, Renjun Gu, Xiangyu Bian, Xin Xu, Xuefeng Xia, Yuchen Liu, Chengjie Jia, Yihuang Gu, Hongru Zhang
Summary: This study evaluated the effects of remote tissue compression on infarct size after myocardial infarction and identified its cardioprotective effects. The cardioprotective signal transmission of remote conditioning involves the release of adenosine, essential for its anti-inflammatory properties through the activation of the cAMP/PKA/NF-kappa B axis.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Medicine, Research & Experimental
Manuela Cabiati, Costanza Salvadori, Giuseppina Basta, Serena Del Turco, Paolo Aretini, Antonella Cecchettini, Silvia Del Ry
Summary: This study analyzed the transcriptional profile of miRNAs and lncRNAs in EVs derived from HepG2 and WRL68 cell lines, revealing lower expression of miR-181a, miR-205, and miR-1323 in HepG2 EVs compared to WRL68, as well as significant correlations between multiple miRNAs and lncRNAs. These findings suggest potential new diagnostic and prognostic biomarkers for HCC.
CLINICAL AND EXPERIMENTAL MEDICINE
(2022)
Article
Endocrinology & Metabolism
Maria Angela Guzzardi, Letizia Guiducci, Daniela Campani, Federica La Rosa, Andrea Cacciato Insilla, Antonietta Bartoli, Manuela Cabiati, Vincenzo De Sena, Silvia Del Ry, Silvia Burchielli, Ferruccio Bonino, Patricia Iozzo
Summary: This study investigated the impact of leptin resistance on fat accumulation and metabolic diseases, focusing on glucose uptake in brown and subcutaneous adipose tissues and the liver. Results showed that in leptin-resistant rats, glucose intolerance was related to hepatic glucose overproduction, playing a role in the pathogenesis of liver inflammatory disease.
INTERNATIONAL JOURNAL OF OBESITY
(2022)
Review
Pharmacology & Pharmacy
Martina Fontanini, Manuela Cabiati, Manuel Giacomarra, Giovanni Federico, Silvia Del Ry
Summary: This review discusses the involvement of long non-coding RNAs (lncRNAs) in obesity and lipid metabolism. By studying these lncRNAs in depth, a better understanding of the causes of obesity can be gained and the potential for using them as therapeutic targets can be explored.
CURRENT PHARMACEUTICAL DESIGN
(2022)
Article
Cell Biology
Rosalinda Madonna, Damiana Pieragostino, Maria Concetta Cufaro, Piero Del Boccio, Angela Pucci, Letizia Mattii, Vanessa Doria, Christian Cadeddu Dessalvi, Riccardo Zucchi, Giuseppe Mercuro, Raffaele De Caterina
Summary: Ponatinib, a tyrosine kinase inhibitor used in chronic myeloid leukaemia, has been shown to have cardiovascular toxicity. This study investigated the mechanisms of sex-related Ponatinib-induced cardiotoxicity and identified rescue strategies using a mouse model. The results demonstrated sex-related differences in the cardiotoxicity of Ponatinib and the involvement of sex hormones.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Rosalinda Madonna, Serena Barachini, Stefania Moscato, Chiara Ippolito, Letizia Mattii, Chiara Lenzi, Carmela Rita Balistreri, Riccardo Zucchi, Raffaele De Caterina
Summary: This study investigated the effects of SGLT2 inhibitors on PON-induced endothelial cell toxicity. The results showed that EMPA and DAPA could reverse the effects of PON on cell viability, cell senescence, and cell tubulization, thereby attenuating PON-induced vascular toxicity.
VASCULAR PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Manuela Cabiati, Martina Fontanini, Manuel Giacomarra, Gianfranco Politano, Emioli Randazzo, Diego Peroni, Giovanni Federico, Silvia Del Ry
Summary: This study aimed to identify inflammatory LncRNAs in obese adolescents and evaluate their expression differences compared to normal-weight adolescents. The results showed that only SNHG12 had significantly lower expression in obese adolescents. Circulating miRNAs showed an increasing trend in obese subjects, except for miR-181a expression. LncRNAs and miRNAs were correlated with some clinical and metabolic parameters.
Article
Biochemistry & Molecular Biology
Silverio Sbrana, Antonella Cecchettini, Luca Bastiani, Nicoletta Di Giorgi, Annamaria Mazzone, Elisa Ceccherini, Federico Vozzi, Chiara Caselli, Danilo Neglia, Alberto Clemente, Arthur J. H. A. Scholte, Oberdan Parodi, Gualtiero Pelosi, Silvia Rocchiccioli
Summary: This study found an association between plaque dense-calcium volume (DCV) in patients with stable coronary artery disease (CAD) and M2-like phenotypic polarization of circulating monocytes, indicating that the functional state of anti-inflammatory monocytes may partly modulate plaque macro-calcification.
Article
Physiology
Michela Novelli, Matilde Masini, Cecilia Vecoli, Stefania Moscato, Niccola Funel, Anna Pippa, Letizia Mattii, Chiara Ippolito, Daniela Campani, Danilo Neglia, Pellegrino Masiello
Summary: eNOS-deficient mice developed insulin resistance and morphological changes in the pancreas, including beta-cell hyperplasia and the appearance of hybrid acinar-beta-cells, under high-fat diet conditions. These changes may be caused by deficient endothelial NO production.
PHYSIOLOGICAL REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Rosetta Ragusa, Silvia Masotti, Veronica Musetti, Silvia Rocchiccioli, Concetta Prontera, Marco Perrone, Claudio Passino, Aldo Clerico, Chiara Caselli
Summary: Cardiac troponins are key elements in the regulation of cardiac excitation-contraction coupling and can be used as biomarkers of acute myocardial infarction. With the use of high sensitivity immunoassay tests, they can also be used as biomarkers for other cardiac conditions. This review discusses the mechanisms of cardiac troponin release in the blood and its potential as a biomarker for cardiac adverse events.
Article
Cardiac & Cardiovascular Systems
Rosalinda Madonna, Stefania Moscato, Maria Concetta Cufaro, Damiana Pieragostino, Letizia Mattii, Piero Del Boccio, Sandra Ghelardoni, Riccardo Zucchi, Raffaele De Caterina
Summary: This study found that empagliflozin can inhibit excessive autophagy in the heart of diabetic patients by suppressing the AMPK/GSK3β signaling pathway, thus protecting the heart from damage.
CARDIOVASCULAR RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Manuela Cabiati, Emioli Randazzo, Letizia Guiducci, Alessandra Falleni, Antonella Cecchettini, Valentina Casieri, Giovanni Federico, Silvia Del Ry
Summary: Exosomes may not play a significant role in transporting the CNP system, but they do carry differentially expressed miRNAs in obese and normal-weight adolescents. These miRNAs show potential correlations with inflammatory/metabolic factors and can serve as biomarkers for identifying obese children and adolescents at risk of developing disease complications in adulthood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Silverio Sbrana, Antonella Cecchettini, Luca Bastiani, Annamaria Mazzone, Federico Vozzi, Chiara Caselli, Danilo Neglia, Alberto Clemente, Arthur J. H. A. Scholte, Oberdan Parodi, Gualtiero Pelosi, Silvia Rocchiccioli
Summary: In this study, the relationship between neutrophil number and phenotype and lipid-rich necrotic core volume (LRNCV) in stable coronary artery disease (CAD) patients was evaluated. The results suggest that increased circulating neutrophils and up-regulation of CD11b marker may contribute to the enlargement of LRNCV, increasing the risk of acute complications in CAD patients.
Letter
Medicine, General & Internal
Chiara Caselli, Rosetta Ragusa, Riccardo Liga, Concetta Prontera, Alessia Gimelli, Arthur Scholte, Juhani Knuuti, Aldo Clerico, Danilo Neglia
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Alessandra Falleni, Stefania Moscato, Giovanni Fulvio, Enza Polizzi, Margherita Bernardeschi, Francesco Bianchi, Valentina Donati, Manuela Cabiati, Chiara Ippolito, Silvia Del Ry, Chiara Baldini, Letizia Mattii
Summary: This study identified the presence of Cx26, Cx32, and Cx43 proteins in human salivary glands and their localization in duct cells, acinar cells, and myoepithelial cells. This suggests that Cxs may play a role in salivary gland dysfunctions.