Article
Materials Science, Multidisciplinary
Sonatan Das, Akanksha Singh, V. Ramgopal Rao, Tapanendu Kundu
Summary: The study focuses on the development of a semiconducting functional polymer with ferroelectric behavior by tuning the stoichiometry ratio of the moieties in order to create a new type of polymer photodetector. The device exhibits photocurrent generation and ferroelectric polarization, making it a promising candidate for single polymer-based field-effect transistors and functional photonic materials.
JOURNAL OF MATERIALS CHEMISTRY C
(2022)
Article
Chemistry, Analytical
Yuqian Yang, Zhiqing Xiao, Lexin Sun, Zitao Feng, Zejingqiu Chen, Weijin Guo
Summary: In this study, a novel protocol was developed to fabricate silk fibroin/off-stoichiometry thiol-ene (OSTE) hybrid microneedle array patches. Silk fibroin, a natural biomaterial, was used as a drug carrier. The drug, insulin, was dissolved in a silk fibroin solution and deposited on a microneedle mold. The resulting silk fibroin needle tips were formed on an OSTE base through replica molding. The silk fibroin concentration had minimal influence on the length of the needle tips, and the mechanical strength of the microneedles was tested.
Article
Materials Science, Multidisciplinary
Neil Convery, Iliyana Samardzhieva, John Moir Stormonth-Darling, Sean Harrison, Gareth J. Sullivan, Nikolaj Gadegaard
Summary: This study demonstrates the use of 3D printing for inlays in injection moulding, reducing the skills, cost, and time required for microfluidic chip fabrication. The authors show that the 3D printed inlays are durable enough to produce a large number of parts and that devices can be designed, manufactured, and tested in a short period of time.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2021)
Article
Engineering, Biomedical
Eleonora Borda, Danashi Imani Medagoda, Marta Jole Ildelfonsa Airaghi Leccardi, Elodie Genevieve Zollinger, Diego Ghezzi
Summary: Off-stoichiometry thiol-ene-epoxy (OSTE+) thermosets have low gas permeability and little absorption of dissolved molecules, allowing for low-temperature dry bonding without surface treatments. They can be manufactured via UV polymerisation and have gained attention for rapid prototyping of microfluidic chips and as a novel material for neural implants due to their mechanical properties and compatibility with standard clean-room processes.
Article
Automation & Control Systems
Tim Deringer, Dietmar Drummer
Summary: This study investigates the impact of mold temperatures and curing times on the bond strength in the interface between epoxy molding compounds and fast-curing epoxy prepregs using an integrative process. Through additional rheological and thermoanalytical analyses, the study aims to identify reasons for any changes in bond strength and establish initial process limits for the potential process window.
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Jingyu Wu, David A. Issadore, Daeyeon Lee
Summary: Controlling surface wetting properties of microfluidic channels is essential for reliable performance. Current patterning methods have limited spatial resolution, making them impractical. In this study, a replica mold fabrication technique inspired by soft lithography was developed to transfer channel geometry and surface wettability from a silicon wafer mold to a co-polymer network replica. This technique was successfully demonstrated in the fabrication of a PFPE-PEG-based microfluidic chip with patterned microchannels for double emulsion generation.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Polymer Science
Ricardo Acosta Ortiz, Roberto Yanez Macias, Jose de Jesus Ku Herrera, Aida Esmeralda Garcia Valdez
Summary: An eco-friendly epoxy/thiol-ene photopolymerization (ETEP) process was used to prepare epoxy bio-composites with jute fabric reinforcement. Thiol groups were introduced to the jute fibers to create a chemically bonded polymeric matrix/fiber system. The resulting bio-composites exhibited improved flexural modulus, strength, deformation at break, and toughness, making them suitable for structural applications.
Article
Polymer Science
Sigita Grauzeliene, Aukse Navaruckiene, Edvinas Skliutas, Mangirdas Malinauskas, Angels Serra, Jolita Ostrauskaite
Summary: This study focuses on enhancing the performance of thermosets by combining photo- and thermal polymerization, using renewable resources instead of petroleum-based materials. By studying thiol-epoxy reactions and examining rheological properties, the most suitable formulations for laser direct writing were selected. The dual cured thermosets showed higher rigidity, tensile strength, and thermal stability, demonstrating their potential for high-resolution 3D printing.
Article
Chemistry, Analytical
Roberts Rimsa, Artis Galvanovskis, Janis Plume, Felikss Rumnieks, Karlis Grindulis, Gunita Paidere, Sintija Erentraute, Gatis Mozolevskis, Arturs Abols
Summary: This study explored the use of OSTE as an alternative material for LOAC prototype development, comparing it to PDMS. Results showed that OSTE had similar effects on membrane particle absorption and enzymatic reactions compared to PDMS, but lower small molecule absorption and light transmission. Optical issues with OSTE may need to be addressed for future LOAC prototypes.
Review
Medicine, General & Internal
Job Komen, Sanne M. van Neerven, Albert van den Berg, Louis Vermeulen, Andries D. van der Meer
Summary: This study explores the potential of using organ-on-chip technology to mimic cancer xenograft models for validating cancer-on-chip models. By reviewing the biological characteristics and comparing the growth of on-chip tumors with xenografts, it shows that organ-on-chip technology is capable of mimicking many aspects of xenograft tumors, yet further development and validation is needed.
Article
Chemistry, Multidisciplinary
Iuliana Ribca, Benedikt Sochor, Stephan V. V. Roth, Martin Lawoko, Michael A. R. Meier, Mats Johansson
Summary: In this study, technical Kraft lignin was fractionated using a sequential solvent fractionation method, and then most of the hydroxyl groups were allylated to obtain highly functional thermoset resins. The lignin fractions exhibited a high degree of functionalization on phenolic (≥95%) and carboxylic acid (≥85%) hydroxyl groups, as well as a significant level of functionalization on aliphatic hydroxyl moieties (43-75%). Thiol-ene chemistry was employed to cross-link the resins, resulting in a high cross-link density due to the abundant allyl functionalities. The performance of these thermosets, with a glass transition temperature (T(g)) between 81 and 95 °C and a storage modulus between 1.9 and 3.8 GPa, was strongly influenced by the thioether content, which is directly related to the allyl content. Morphology at the nanoscale of the lignin fractions and lignin-based thermosets was investigated using wide-angle X-ray scattering measurements, revealing the presence of sandwich (4.1-4.7 Å) and T-shaped (5.5-7.2 Å) π-π stacking interactions. The introduction of allyl functionalities weakened the T-shaped π-π stacking interactions. A new signal, corresponding to a distance of approximately 3.5 Å, was observed in lignin-based thermosets, which was attributed to a thioether organized structure. Additionally, a lignin superstructure with a distance/size corresponding to 7.9-17.5 Å was observed in all samples.
Article
Chemistry, Analytical
Filippo Amadeo, Prithviraj Mukherjee, Hua Gao, Jian Zhou, Ian Papautsky
Summary: Soft lithography for microfluidic devices is popular due to its simplicity and low cost, but PDMS masters can be expensive and fragile. This study presents an optimized method using PC sheets on PDMS molds for replication, successfully replicating various microfluidic devices with different aspect ratios. It is a cost-effective, fast, and simple approach for long-term preservation of soft lithography masters.
Article
Biophysics
Shan Liu, Shikha Kumari, Hongyi He, Parichita Mishra, Bhisham Narayan Singh, Divakar Singh, Sutong Liu, Pradeep Srivastava, Chenzhong Li
Summary: As a simulation of human cells, tissues, organs, and systems, Organ-on-a-Chip (OOC) has ethical advantages and development potential over animal experiments. Advancements in 3D cell biology and engineering, chip materials, and 3D printing have facilitated the connection of multi-organs-on-chips for simulation and the development of new drug screening platforms. The success of organ model modeling is crucial, and this paper provides a comprehensive review and discussion of detection and evaluation technologies in organ-on-a-chip research.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemical Research Methods
Nan Lu, Nickolaj J. Petersen, Andreas C. Kretschmann, Jorg P. Kutter
Summary: Non-aqueous capillary electrophoresis on a thiol-ene microfluidic chip coupled with ESI-MS offers a versatile system for the analysis of lipophilic compounds in various organic solvents, showing good stability and separation efficiency. The integration of ESI emitter on the microchip demonstrated stable signal with high separation efficiency using ethanol-based buffers. The direct coupling of non-aqueous electrophoresis to mass spectrometry allows for fast analysis with good sensitivities for hydrophobic compounds.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Caroline Despicht, Cecilie H. Munkboel, Hua Nee Chou, Peter Ertl, Mario Rothbauer, Jorg P. Kutter, Bjarne Styrishave, Andreas Kretschmann
Summary: The development of cell-based microfluidic assays offers new opportunities in toxicity testing through integration of new functionalities, automation, and high throughput. This study aimed to miniaturize the H295R steroidogenesis assay from the well-plate format to a microfluidic chip. PDMS and thiol-ene were tested as potential chip materials, and thiol-ene showed more favorable properties for H295R cell culture. A microfluidic chip design and cell seeding procedure were successfully developed, achieving repeatable and uniform cell distribution.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Patrick Schuller, Mario Rothbauer, Sebastian R. A. Kratz, Gregor Hoell, Phillipp Taus, Markus Schinnerl, Jakob Genser, Neus Bastus, Oscar H. Moriones, Victor Puntes, Berthold Huppertz, Monika Siwetz, Heinz Wanzenboeck, Peter Ertl
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Chemistry, Multidisciplinary
Hadi Hashemzadeh, Abdollah Allahverdi, Mosslim Sedghi, Zahra Vaezi, Tahereh Tohidi Moghadam, Mario Rothbauer, Michael Bernhard Fischer, Peter Ertl, Hossein Naderi-Manesh
Article
Spectroscopy
Mario Rothbauer, Christoph Eilenberger, Sarah Spitz, Barbara Bachmann, Jasmin Pajenda, Andreas Schwaighofer, Gregor Hoell, Palle Steen Helmke, Yvonne Kohl, Bernhard Lendl, Peter Ertl
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2020)
Article
Cell Biology
Katharina M. Pichler, Anita Fischer, Juergen Alphonsus, Catharina Chiari, Sebastian Schmidt, Michael Kenn, Wolfgang Schreiner, Daniela Weinmann, Mario Rothbauer, Reinhard Windhager, Hans-Joachim Gabius, Stefan Toegel
Summary: Galectin-4 (Gal-4) is a galactose-binding protein that is upregulated in osteoarthritis, leading to the upregulation of pro-degradative/-inflammatory proteins in chondrocytes. This study suggests that targeting galectin activity may be a potential therapeutic approach in OA progression.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Cell Biology
Hadi Hashemzadeh, Ali Hamad Abd Kelkawi, Abdollah Allahverdi, Mario Rothbauer, Peter Ertl, Hossein Naderi-Manesh
Summary: The inadequacy of animal models has led to the development of improved in vitro models, such as 3D multicellular spheroids, for drug efficacy assessments. While these models provide physiological relevance and meet high-throughput and low-cost requirements for drug discovery, their application in nanotoxicity testing is still in its early stages due to limited penetration and uptake rates. Comparative studies between 2D monolayer cultures and 3D spheroids revealed distinct cellular responses to gold nanowires (AuNWs) treatment, emphasizing the need for more physiologically relevant tissue models for investigating nanomaterial-biology interactions.
Article
Pharmacology & Pharmacy
Christoph Eilenberger, Mario Rothbauer, Konstanze Brandauer, Sarah Spitz, Eva-Kathrin Ehmoser, Seta Kuepcue, Peter Ertl
Summary: Stem cell technology and embryonic stem cell models are crucial for studying neurogenesis and early brain development. However, the lack of standardization and comparability in the establishment of embryoid bodies (EBs) hinders the broader application of stem cell technology. This study focuses on optimizing differentiation protocols and the size of EBs to enhance the reliability of neurodevelopmental in vitro models.
Article
Biochemistry & Molecular Biology
Helene Stuetz, Eva I. Reihs, Winfried Neuhaus, Maren Pflueger, Harald Hundsberger, Peter Ertl, Christian Resch, Gerald Bauer, Guenter Povoden, Mario Rothbauer
Summary: Airborne engineered nanomaterials are by-products of industry and are considered to be important environmental toxins that can pose health risks to humans and animals. The main routes of exposure to airborne nanoparticles are through nasal and/or oral inhalation, which allow the particles to enter the bloodstream and spread throughout the body. Mucosal barriers in the nose, mouth, and lungs play a key role in preventing the translocation of nanoparticles, but there is limited understanding of their tolerance to nanoparticle exposures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Mario Rothbauer, Ruth A. Byrne, Silvia Schobesberger, Isabel Olmos Calvo, Anita Fischer, Eva Reihs, Sarah Spitz, Barbara Bachmann, Florian Sevelda, Johannes Holinka, Wolfgang Holnthoner, Heinz Redl, Stefan Toegel, Reinhard Windhager, Hans P. Kiener, Peter Ertl
Summary: Research established a tissue coculture model mimicking rheumatoid arthritis, simulating the reciprocal cross talk between synovial and chondral organoids. Results showed that when chondral organoids were co-cultivated with synovial organoids, they induced a higher degree of cartilage physiology and architecture, with differential cytokine response.
Article
Chemistry, Analytical
Ali Khodayari Bavil, Drago Sticker, Mario Rothbauer, Peter Ertl, Jungkyu Kim
Summary: An impedimetric biosensor platform was developed to enhance the sensitivity of immunoassays using microparticles as labels by measuring electrical impedance changes. Through testing various microparticles, it was found that magnetic microparticles provided high signal enhancement and stability on the sensor array. By incorporating magnetic microparticles, over ten times improvement of LOD was achieved in sandwich immunoassays.
Article
Chemistry, Analytical
Cristian Zanetti, Sarah Spitz, Emanuel Berger, Silvia Bolognin, Lisa M. Smits, Philipp Crepaz, Mario Rothbauer, Julie M. Rosser, Martina Marchetti-Deschmann, Jens C. Schwamborn, Peter Ertl
Summary: This study developed a novel electrochemical sensing approach to detect dopamine in human midbrain organoids, allowing for monitoring differences in dopamine release between healthy and Parkinson's disease-specific midbrain organoids. The practical feasibility of the sensing approach for drug screening applications and personalized disease modeling was demonstrated.
Article
Biochemical Research Methods
Michaela Purtscher, Mario Rothbauer, Sebastian Rudi Adam Kratz, Andrew Bailey, Peter Lieberzeit, Peter Ertl
Summary: Detection, quantification and monitoring of virus-host cell interactions play a crucial role in evaluating the safety of pharmaceutical products. Regulatory agencies recommend viral clearance studies to assess the efficiency of production processes in removing viruses. A microfluidic single step assay can significantly reduce assay time to 3 to 4 days, and continuous fluid flow can enhance virus-cell interactions.
Article
Biochemical Research Methods
Mario Rothbauer, Gregor Hoell, Christoph Eilenberger, Sebastian R. A. Kratz, Bilal Farooq, Patrick Schuller, Isabel Olmos Calvo, Ruth A. Byrne, Brigitte Meyer, Birgit Niederreiter, Seta Kuepcue, Florian Sevelda, Johannes Holinka, Oliver Hayden, Sandro F. Tedde, Hans P. Kiener, Peter Ertl
Article
Multidisciplinary Sciences
Patrick Schuller, Mario Rothbauer, Christoph Eilenberger, Sebastian R. A. Kratz, Gregor Hoell, Philipp Taus, Markus Schinnerl, Jakob Genser, Peter Ertl, Heinz Wanzenboeck
Article
Biochemical Research Methods
Mario Rothbauer, Verena Charwat, Barbara Bachmann, Drago Sticker, Richard Novak, Heinz Wanzenboeck, Richard A. Mathies, Peter Ertl
Article
Multidisciplinary Sciences
Christoph Eilenberger, Mario Rothbauer, Eva-Kathrin Ehmoser, Peter Ertl, Seta Kuepcue
SCIENTIFIC REPORTS
(2019)
