期刊
LAB ON A CHIP
卷 15, 期 2, 页码 557-562出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4lc01277a
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资金
- EPSRC [EP/J017566/1, EP/K038648/1, EP/G00465X/1]
- BBSRC [BB/F013167/1]
- EPSRC Centre for Doctoral Training Studentship from the Institute of Chemical Biology (Imperial College London)
- EPSRC [EP/K039946/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F013167/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/G00465X/1, 1230518, EP/J017566/1, EP/K038648/1, EP/K039946/1] Funding Source: researchfish
Giant unilamellar vesicles (GUVs) have a wide range of applications in biology and synthetic biology. As a result, new approaches for constructing GUVs using microfluidic techniques are emerging but there are still significant shortcomings in the control of fundamental vesicle structural parameters such as size, lamellarity, membrane composition and internal contents. We have developed a novel microfluidic platform to generate compositionally-controlled GUVs. Water-in-oil (W/O) droplets formed in a lipidcontaining oil flow are transferred across an oil-water interface, facilitating the self-assembly of a phospholipid bilayer. In addition, for the first time we have studied the mechanical properties of the resultant lipid bilayers of the microfluidic GUVs. Using fluctuation analysis we were able to calculate the values for bending rigidity of giant vesicles assembled on chip and demonstrate that these correlate strongly with those of traditional low throughput strategies such as electroformation.
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