Article
Biochemical Research Methods
Junyu Ma, Yihui Wu, Yongshun Liu, Yuan Ji, Mei Yang, Hongquan Zhu
Summary: The centrifugal microfluidic chip transforms non-steady flow into locally steady flow with a flow rectifier, which improves separation purity and recovery ratio of target particles. It can be used to separate particles of differing sizes, including rare tumor cells. Experimental results show that the chip has achieved good separation purity and recovery ratio.
Article
Chemistry, Analytical
Shih-Jui Huang, Chia-Ming Chang, Yen-Ta Lu, Cheng-Hsien Liu
Summary: This study developed an integrated microfluidic biochip to separate target tumor cells and clusters from whole blood using a two-stage sorting mechanism. The chip achieved high recovery rates and purity in separating tumor cell clusters, making it suitable for cancer research and clinical applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Mechanics
Md Ehtashamul Haque, Amirali Matin, Xu Wang, Maiwenn Kersaudy-Kerhoas
Summary: The study reveals that syringe pump fluctuations can have a negative impact on microfluidic separation, especially in devices based on the cell-free layer concept. It was found that pressure fluctuations tend to stabilize at higher flow rates. This work provides insights for the design and improvement of microfluidic systems in resisting pulsating or fluctuating flow conditions.
Review
Chemistry, Analytical
Sheng Yan, Dan Yuan
Summary: The development of microfluidic technologies, particularly microflow cytometry with 3D focusing capabilities, has shown great promise in single-cell analysis, offering a wide range of applications in biology and medicine.
Article
Biophysics
Jie Wang, Xianmeng Meng, Min Yu, Xin Li, Zhezhou Chen, Rui Wang, Jin Fang
Summary: This study designed an integrated microfluidic system to differentiate the invasive potential of circulating tumor cells (CTCs) for more accurate metastasis prediction. The system successfully detected functional CTCs derived from different types of cancer patients using whole blood without any sample pretreatment process. The functional CTCs exhibited stronger invasiveness and were associated with metastases.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemical Research Methods
Moritz Leuthner, Oliver Hayden
Summary: Lubrication of the syringe pump guide rods after cleaning significantly reduces flow rate fluctuations, especially at low pressure drops.
Article
Biophysics
Gaolin Li, Yuan Ji, Yihui Wu, Yongshun Liu, Huan Li, Yimeng Wang, Mingbo Chi, Hongyan Sun, Hongquan Zhu
Summary: In this study, a multistage microfluidic CTCs sorting method was presented, which involved size-based sorting using a two-array DLD chip, purification of CTCs mixed with leukocytes using a stiffness-based cone channel chip, and cell type identification using Raman techniques. The entire sorting process was label free, highly pure, high-throughput and efficient. The system developed with four parallelized DMC two-array DLD chips achieved a sample processing rate of 2.5 mL per minute with a recovery efficiency of 96.30 ± 2.10% and a purity of 98.25 ± 2.48%. A cone channel chip based on solid and hydrodynamic coupled analysis was developed to improve the purity of CTCs mixed with leukocytes by 1.8-fold.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Sunghak Choi, Bongsu Kang, Shogo Taguchi, Hiroshi Umakoshi, Keesung Kim, Moon Kyu Kwak, Ho-Sup Jung
Summary: A novel method for DMPC/DHPC bicelle synthesis based on a microfluidic device without heating and freezing processes is proposed, enabling the continuous manufacturing of bicelles. Experimental and analytical studies reveal critical lipid concentration and mixing time in the microfluidic system, as well as a linear relation between the actual composition of bicelle and initial lipid ratio. This method allows for controlled size of bicelles.
Article
Chemistry, Analytical
Yu Wang, Dong-Fei Wang, Hui-Feng Wang, Jian-Wei Wang, Jian-Zhang Pan, Xiao-Gang Guo, Qun Fang
Summary: This study developed a microfluidic robot platform for sorting rare target cells from complex clinical blood samples, achieving high efficiency and purity through a novel multi-step sorting strategy. The platform automates the identification, capture, and droplet generation of rare cells, demonstrating the feasibility and potential application in the sorting and analysis of specific rare cells like circulating endothelial progenitor cells (CEPCs) in human blood.
Article
Biotechnology & Applied Microbiology
Jenny Kirsch, Kerstin Heinrich, Daniel Kage, Johannes Glaab, Benjamin Grothe, Konrad V. Volkmann, Toralf Kaiser
Summary: Aseptic cell sorting is challenging, especially without a sterile environment. Chemical cleaning procedures for cell sorters may not be effective. This study presents a method using UV-C light to decontaminate the sheath fluid and achieve a significant reduction in bacteria.
ENGINEERING IN LIFE SCIENCES
(2022)
Article
Polymer Science
Xiaozhi Ouyang, Cheng Huang, Sha Cheng, Pengchao Zhang, Wen Chen
Summary: In this study, a microfluidic-based strategy for the continuous fabrication of ultrathin hydrogel films was proposed. This strategy allows for precise control over the thickness of the hydrogel films and can be scaled up. The method shows advantages in producing high uniformity and structural integrity of the hydrogel films without the need for supporting substrates and sophisticated equipment. Therefore, this strategy provides a facile route to produce advanced thin polymer films and promotes their applications in biosensors and wearable electronics.
Review
Chemistry, Analytical
Yupan Wu, Yingqi Meng
Summary: This paper reviews emerging tools enabled by microfluidic technologies for cell manipulation, including sorting and single cell trapping, and even single cell characterization. The focus is on electric field, mechanical, and fluorescence-based methods for single cell analysis and characterization, as well as the integrated microfluidic devices. The authors further discuss future commercialization and research prospects, highlight current bottlenecks, and provide their perspective on the prospects for microfluidic cell manipulation.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zhihang Yu, Jing Jin, Siyuan Chen, Lingling Shui, Huaying Chen, Liuyong Shi, Yonggang Zhu
Summary: This paper presents a smart droplet microfluidic system that allows for single-cell selective lysis and real-time sorting. By applying voltage and injection pressure to the oil-water interface, the efficiency of droplet injection is greatly improved and the cell lysis solution can be selectively injected into target single-cell droplets. The system function is explored by introducing complex cell samples, achieving a high success rate in treating target cells.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Yishen Tian, Rong Hu, Guangshi Du, Na Xu
Summary: Adoptive cell therapy (ACT) is a personalized therapy that has shown great success in treating hematologic malignancies and potential applications for solid tumors. Microfluidic chips have advantages of high throughput, low cell damage, and fast amplification rates, and can simplify ACT preparation steps and reduce costs.
Article
Chemistry, Analytical
Keondo Lee, Seong-Eun Kim, Seokho Nam, Junsang Doh, Wan Kyun Chung
Summary: Image-based cell sorting is important in biological research. This study presents an upgraded sorting system with added features to facilitate real-time sorting of cells or particles.
