Article
Biochemical Research Methods
Jake A. Melby, Willem J. de Lange, Jianhua Zhang, David S. Roberts, Stanford D. Mitchell, Trisha Tucholski, Gina Kim, Andreas Kyrvasilis, Sean J. McIlwain, Timothy J. Kamp, J. Carter Ralphe, Ying Ge
Summary: The study established an integrated method that allows sequential assessment of functional properties and top-down proteomics from the same hiPSC-ECT construct, revealing a strong correlation between contractile parameters and phosphorylation levels of alpha-tropomyosin. This integrated approach enables a holistic analysis of hiPSC-ECTs for applications in disease modeling, cardiotoxicity, and drug discovery.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Amit Gruber, Oded Edri, Shany Glatstein, Idit Goldfracht, Irit Huber, Gil Arbel, Amira Gepstein, Snizhanna Chorna, Lior Gepstein
Summary: This study aimed to develop high-resolution optogenetic approaches to modulate electrical activity in 2D and 3D cardiac tissue models derived from human induced pluripotent stem cell cardiomyocytes. The results demonstrated the ability to control the conduction and contractile properties of cardiac cells using optogenetics, and to use light-controllable techniques for cardiac resynchronization therapy and arrhythmia termination. This approach has important applications in cardiac physiology, pathophysiology, and arrhythmia treatment.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Cell & Tissue Engineering
Somayeh Pouyanfard, Nairika Meshgin, Luisjesus S. Cruz, Karin Diggle, Hamidreza Hashemi, Timothy Pham, Manuel Fierro, Pablo Tamayo, Andrea Fanjul, Tatiana Kisseleva, Dan S. Kaufman
Summary: The study shows that human iPSC-derived macrophages can effectively reduce gene expression and pathological markers of liver fibrosis, offering a promising cell therapy approach to ameliorate fibrosis.
Review
Cell Biology
Benjamin Theisen, Austin Holtz, Viswanathan Rajagopalan
Summary: Brugada Syndrome, a cardiac channelopathy, is associated with gene mutations and noncoding RNAs, leading to life-threatening arrhythmias. Studies have shown that the majority of loci associated with electrophysiological traits are located in noncoding regulatory regions, affecting gene expression dosage and cardiac ion channel function.
Article
Biochemistry & Molecular Biology
Martha E. Floy, Sophie E. Givens, Oriane B. Matthys, Taylor D. Mateyka, Charles M. Kerr, Alexandra B. Steinberg, Ana C. Silva, Jianhua Zhang, Ying Mei, Brenda M. Ogle, Todd C. McDevitt, Timothy J. Kamp, Sean P. Palecek
Summary: The study explores the developmental lineage and functional properties of cardiac fibroblasts derived from human pluripotent stem cells, highlighting their potential in modeling fibrotic diseases and regenerative therapies in the context of heart function. The differences in characteristics between epicardial and second heart field cardiac fibroblasts provide insights for in vitro model selection and therapeutic applications.
Article
Medicine, Research & Experimental
Filomain Nguemo, Erastus Nembu Nembo, Michelle Vanessa Kamga Kapchoup, Franz Enzmann, Juergen Hescheler
Summary: The study found that QuinoMit Q10-Fluid inhibits the proliferation of pluripotent stem cells and has less effect on cardiomyogenesis. However, clusters containing CMs generated under QuinoMit Q10-Fluid (1:100) showed a significantly increased beating rate. Additionally, CMs pretreated with QuinoMit exhibited a protective effect against H2O2-induced arrhythmia compared to untreated CMs.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Yin-Yu Lam, Chun-Ho Chan, Lin Geng, Nicodemus Wong, Wendy Keung, Yiu-Fai Cheung
Summary: Cardiomyocytes can be derived from hiPSC lines, but the efficacy varies. By performing single cell transcriptomics, researchers identified cardiac and extra-cardiac lineages during differentiation. They found APLNR as a surface marker for in vitro cardiac progenitors, and using this marker, they isolated and differentiated cardiac progenitors from multiple hiPSC lines. This study has potential applications in studying cardiac development, disease modeling, and cardiac regeneration.
Article
Multidisciplinary Sciences
Martta Hakli, Joose Kreutzer, Antti-Juhana Maki, Hannu Valimaki, Henna Lappi, Heini Huhtala, Pasi Kallio, Katriina Aalto-Setala, Mari Pekkanen-Mattila
Summary: Ischemic heart disease is a major cause of death worldwide, and reperfusion therapy can cause further damage initially. A human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) platform was developed to model ischemia-reperfusion, showing changes in beating frequency and adaptations to oxygen conditions. The study also observed disorganization of sarcomere structures and increased expression of glucose transporter 1 gene, indicating the potential of hiPSC-CMs in modeling ischemia-reperfusion injury despite their immature phenotype.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Alina Constantin, Ioana Karla Comarita, Nicoleta Alexandru, Alexandru Filippi, Florina Bojin, Mihaela Gherghiceanu, Alexandra Vilcu, Miruna Nemecz, Loredan Stefan Niculescu, Virgil Paunescu, Adriana Georgescu
Summary: This study investigated the effects of stem cell-derived extracellular vesicles (EVs) on cardiac hypertrophy. The results showed that these EVs could reduce the expression of hypertrophic markers and molecules associated with inflammation in hypertrophic cardiomyocytes. The findings suggest that EVs have the potential to act as therapeutic mediators to reduce cardiac hypertrophy and subsequent cardiovascular events.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Devansh Agarwal, Rian Kuhns, Christos N. N. Dimitriou, Emmalyn Barlow, Karl J. J. Wahlin, Ray A. A. Enke
Summary: Researchers utilized human pluripotent stem cell-derived 3D retinal organoids and Next Generation Sequencing to explore the gene regulation and cell fate determination in human retinal development, providing valuable reference for vision research.
Article
Cell Biology
Bimal Gurung, Gary Tse, Wendy Keung, Ronald A. Li, Wing Tak Wong
Summary: The study shows that hypokalaemia has pro-arrhythmic effects on hvCAS, leading to changes in conduction velocity, repolarization, refractoriness, and calcium handling. This bioengineered platform provides a useful tool for investigating electrophysiological substrates and conducting arrhythmia screening.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Engineering, Biomedical
Xinyue Chu, Mingyu Wang, Xiaoyan Qiu, Yun Huang, Tong Li, Edward Otieno, Na Li, Li Luo, Xiong Xiao
Summary: Although 3D cardiac micro-tissue technology is promising, there are challenges to overcome such as incomplete tissue microarchitecture and poor reproducibility. However, optimizing construction and improving cell functions can lead to potential applications in drug screening and regeneration therapy for heart diseases.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Chemistry, Multidisciplinary
He Xu, Ge Liu, Jixing Gong, Ying Zhang, Shanshan Gu, Zhongjun Wan, Pengcheng Yang, Yage Nie, Yinghan Wang, Zhan-peng Huang, Guanzheng Luo, Zhongyan Chen, Donghui Zhang, Nan Cao
Summary: This study demonstrates the cardiotoxicity of certain antiviral drugs in clinically relevant concentrations using human pluripotent stem cell-derived cardiomyocytes and human engineered heart tissue models. It shows that these drugs can induce cell death, sarcomere disarray, and dysregulation of calcium handling and contraction. Furthermore, it identifies potential drug candidates that can ameliorate the cardiotoxicity of one of these drugs.
Review
Biochemistry & Molecular Biology
Mandeep Kumar, Nhung Thi Phuong Nguyen, Marco Milanese, Giambattista Bonanno
Summary: Astrocytes play a crucial role in neurodegenerative disorders, and the differentiation of human iPSCs into astrocytes has opened up new possibilities for disease modeling and drug screening. This advancement in research offers a promising avenue for exploring treatment strategies for neurodegenerative diseases.
Article
Gastroenterology & Hepatology
Tadahiro Shinozawa, Masaki Kimura, Yuqi Cai, Norikazu Saiki, Yosuke Yoneyama, Rie Ouchi, Hiroyuki Koike, Mari Maezawa, Ran-Ran Zhang, Andrew Dunn, Autumn Ferguson, Shodai Togo, Kyle Lewis, Wendy L. Thompson, Akihiro Asai, Takanori Takebe
Summary: This study established a screening model based on human liver organoids (HLO) for analyzing drug-induced liver injury (DILI) pathology, providing unique advantages at the organoid resolution. Through this model, a multi-readout organoid analysis was successfully developed to measure viability, cholestatic and/or mitochondrial toxicity, showing high predictive values for marketed drugs.
Article
Engineering, Biomedical
Hui Yang, Lai Wei, Chen Liu, Weiyi Zhong, Bin Li, Yuncan Chen, Rui Han, Jiexian Zhuang, Jianxun Qu, Hongyue Tao, Haiyan Chen, Chen Xu, Qianqian Liang, Chao Lu, Ruizhe Qian, Sifeng Chen, Wenshuo Wang, Ning Sun
ACTA BIOMATERIALIA
(2019)
Article
Physiology
Baiping Cui, Yufan Zheng, Xinyan Zhou, Jiaqi Zhu, Jiexian Zhuang, Qianqian Liang, Chen Xu, Wei Sheng, Guoying Huang, Lina Luan, Ning Sun
FRONTIERS IN PHYSIOLOGY
(2019)
Article
Engineering, Biomedical
Xiao Lin, Yue Liu, Aobing Bai, Huanhuan Cai, Yanjie Bai, Wei Jiang, Huilin Yang, Xinhong Wang, Lei Yang, Ning Sun, Huajian Gao
NATURE BIOMEDICAL ENGINEERING
(2019)
Review
Chemistry, Multidisciplinary
Dantong (Danielle) Huang, Sarah B. Gibeley, Cong Xu, Yang Xiao, Ozgenur Celik, Henry N. Ginsberg, Kam W. Leong
ADVANCED FUNCTIONAL MATERIALS
(2020)
Article
Cell Biology
Ermin Li, Xiuya Li, Jie Huang, Chen Xu, Qianqian Liang, Kehan Ren, Aobing Bai, Chao Lu, Ruizhe Qian, Ning Sun
Article
Integrative & Complementary Medicine
Sining Wang, Huihua Chen, Yufan Zheng, Zhenyu Li, Baiping Cui, Pei Zhao, Jiali Zheng, Rong Lu, Ning Sun
Article
Materials Science, Biomaterials
Cong Xu, Min Zhang, Wenwen Chen, Lei Jiang, Chunying Chen, Jianhua Qin
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2020)
Article
Multidisciplinary Sciences
Xinyun Chen, Ying Liu, Chen Xu, Lina Ba, Zhuo Liu, Xiuya Li, Jie Huang, Ed Simpson, Hongyu Gao, Dayan Cao, Wei Sheng, Hanping Qi, Hongrui Ji, Maria Sanderson, Chen-Leng Cai, Xiaohui Li, Lei Yang, Jie Na, Kenichi Yamamura, Yunlong Liu, Guoying Huang, Weinian Shou, Ning Sun
Summary: The RNA-binding protein Quaking (QKI) plays a crucial role in cardiac development and contractile physiology through its regulation of alternative splicing in genes involved in Z-disc formation and contractile physiology. Deficiency of QKI results in the failure of cardiomyocytes to mature into functional cells, highlighting its importance in cardiac pathophysiology.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Jie Huang, Yang Liu, Jia-xin Chen, Xin-ya Lu, Wen-jia Zhu, Le Qin, Zi-xuan Xun, Qiu-yi Zheng, Er-min Li, Ning Sun, Chen Xu, Hai-yan Chen
Summary: Harmine exhibits protective effects on cardiac hypertrophy, reducing cell enlargement, restoring calcium handling and contractility, and downregulating hypertrophy-related genes. It also inhibits NF-kappa B phosphorylation and inflammatory cytokines, suggesting it as a promising therapeutic agent for cardiac hypertrophy independent of blood pressure modulation.
ACTA PHARMACOLOGICA SINICA
(2022)
Letter
Cell Biology
Bin Li, Yongkun Zhan, Qianqian Liang, Chen Xu, Xinyan Zhou, Huanhuan Cai, Yufan Zheng, Yifan Guo, Lei Wang, Wenqing Qiu, Baiping Cui, Chao Lu, Ruizhe Qian, Ping Zhou, Haiyan Chen, Yun Liu, Sifeng Chen, Xiaobo Li, Ning Sun
Article
Medicine, General & Internal
Xiuya Li, Fei Gao, Xiaochen Wang, Qianqian Liang, Aobing Bai, Zhuo Liu, Xinyun Chen, Ermin Li, Sifeng Chen, Chao Lu, Ruizhe Qian, Ning Sun, Ping Liang, Chen Xu
Summary: Knockout of the transcription factor E2A increased the proportion of nodal-like cells in hESC-derived CMs, indicating a potential role of E2A in enhancing the efficiency of generating sinoatrial node-like cardiomyocytes from human pluripotent stem cells in the future.
Article
Cell Biology
Bin Li, Yifan Guo, Yongkun Zhan, Xinyan Zhou, Yongbo Li, Chao Zhao, Ning Sun, Chen Xu, Qianqian Liang
Summary: The deficiency of XIN may play a critical role in the development of DCM induced by TNNT2 mutation, and overexpression of XINB can partially reverse cardiac dysfunction and fibrosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yanchang Li, Qiuyan Lan, Yuan Gao, Cong Xu, Zhongwei Xu, Yihao Wang, Lei Chang, Junzhu Wu, Zixin Deng, Fuchu He, Daniel Finley, Ping Xu
Article
Biochemistry & Molecular Biology
Yufan Zheng, Baiping Cui, Wenrui Sun, Sining Wang, Xu Huang, Han Gao, Fei Gao, Qian Cheng, Limin Lu, Yanpeng An, Xiaobo Li, Ning Sun
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2020)
Article
Medicine, Research & Experimental
Meng Xiang, Meng Lu, Jing Quan, Ming Xu, Dan Meng, Anfeng Cui, Ning Li, Yingying Liu, Peng Lu, Xueling Kang, Xiaokai Wang, Ning Sun, Meng Zhao, Qiujuan Liang, Lili Le, Xinhong Wang, Jianyi Zhang, Sifeng Chen