期刊
HEARING RESEARCH
卷 240, 期 1-2, 页码 102-111出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.heares.2008.03.008
关键词
RT-PCR; immunocytochemistry; confocal microscopy; Belgian Waterslager canary; hearing; acoustic trauma; genetic hearing loss
资金
- NIDCD NIH HHS [R01 DC001372, R01 DC001372-11, P30 DC004664, P30-DC04664, P30 DC004664-04, DC01372] Funding Source: Medline
- PHS HHS [NIDCD 000436] Funding Source: Medline
WD40 repeat 1 protein (WDR1) was first reported in the acoustically injured chicken inner ear, and bio-informatics revealed that WDR1 has numerous WD40 repeats, important for protein-protein interactions. It has significant homology to actin interacting protein 1 (Aip1) in several lower species such as yeast, roundworm, fruitfly and frog. Several studies have shown that Aip1 binds cofilin/actin depolymerizing factor, and that these interactions are pivotal for actin disassembly via actin filament severing and actin monomer capping. However, the role of WDR1 in auditory function has yet to be determined. WDR1 is typically restricted to hair cells of the normal avian basilar papilla, but is redistributed towards supporting cells after acoustic overstimulation, suggesting that WDR1 may be involved in inner ear response to noise stress. One aim of the present study was to resolve the question as to whether stress factors, other than intense sound, could induce changes in WDR1 presence in the affected avian inner ear. Several techniques were used to assess WDR1 presence in the inner ears of songbird strains, including Belgian Waterslager (BW) canary, an avian strain with degenerative hearing loss thought to have a genetic basis. Reverse transcription, followed by polymerase chain reactions with WDR1-specific primers, confirmed WDR1 presence in the basilar papillae of adult BW, non-BW canaries, and zebra finches. Confocal microscopy examinations, following immunocytochemistry with anti-WDR1 antibody, localized WDR1 to the hair cell cytoplasm along the avian sensory epithelium. In addition, little, if any, staining by anti-WDR1 antibody was observed among supporting cells in the chicken or songbird ear. The present observations confirm and extend the early findings of WDR1 localization in hair cells, but not in supporting cells, in the normal avian basilar papilla. However, unlike supporting cells in the acoustically damaged chicken basilar papilla, the inner ear of the BW canary showed little, if any, WDR1 upregulation in supporting cells. This may be due to the fact that the BW canary already has established hearing loss and/or to the possibility that the mechanism(s) involved in BW hearing loss may not be related to WDR1. (C) 2008 Elsevier B.V. All rights reserved.
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