Angiopoietin-like 4 dictates the day-night rhythm of metabolic brown adipose tissue activity

作者

Robin Van Eenige¹ , Wietse In Het Panhuis¹ , Milena Schönke¹ , Céline Jouffe² , Thomas Devilee¹ , Ricky Siebeler¹ , Trea Streefland¹ , Hetty Sips¹ , Amanda Pronk¹ , Ruben Vorderman³ , Hailiang Mei³ , Jan Bert Van Klinken⁴ , Michel Van Weeghel⁴ , Nina Uhlenhaut² , Sander Kersten⁵ , Patrick Rensen¹ , Sander Kooijman¹

1. Dept. Medicine, Div. Endocrinology Einthoven Lab. for Exp. Vasc. Med., LUMC, Leiden, The Netherlands
2. Inst. for Diab. and Endo., Helmholtz Diab. Center and German Center for Diab. Res., Munich, Germany
3. Dept. Biomedical Data Sciences, LUMC, Leiden, The Netherlands
4. Laboratory Genetic Metabolic Diseases, Amsterdam UMC, Amsterdam, The Netherlands
5. Nutr., Metab. and Genom. Group, Div. Human Nutr., Wageningen University, Wageningen, The Netherlands

会议/活动

Dutch Translational Metabolism Conference, June 2022 (Wageningen, Netherlands)

海报摘要

Brown adipose tissue (BAT) burns fatty acids (FAs) to produce heat, and interestingly shows a strong day-night rhythm in glucose and triglyceride (TG)-derived FA-uptake (Van den Berg, Cell Rep 2018), peaking around wakening. Here we aimed to gain insight in the diurnal regulation of metabolic BAT activity. BAT was collected from chow-fed male C57BL/6J mice at 3-hour intervals throughout the day, and RNA-sequencing, chromatin immunoprecipitation (ChIP)-sequencing, and lipidomics analyses were performed. Knockout and overexpression models were used to study causal relationships in diurnal lipid handling by BAT. Our findings highlight involvement of PPARγ and a crucial role of angiopoietin-like 4 (ANGPTL4) in mediating the day-night rhythm of TG-derived FA-uptake by BAT, and implicate that time of day should be considered when studying the metabolic benefits of targeting brown fat.

关键词

Angiopoietin-like 4, Brown adipose tissue, Circadian/diurnal rhythms, Lipoprotein lipase, Peroxisome proliferator-activated receptor gamma, Transcriptomics

研究领域

Molecular and Cellular Biosciences, Medicine

参考文献

1. Khedoe, J Lipid Res 2015
2. Becher, Nat Med 2021
3. Herz, Diabetes 2021
4. Van den Berg, Cell Rep 2018
5. Van Eenige, Mol Metab 2022

基金

暂无数据

补充材料

暂无数据

附加信息

利益冲突

No competing interests were disclosed.

数据可用性声明

The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.

知识共享许可协议

Copyright © 2023 Van Eenige et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.