Ubiquitination of bacterial surface proteins act as novel innate pathogen sensing strategy

作者

Shruti Apte¹ , Smita Bhutda¹ , Sourav Ghosh¹ , Anirban Banerjee¹

1. Indian Institute of Technology Bombay

会议/活动

Innate Immunity in Host-pathogen Interaction, July 2022 (虚拟会议)

海报摘要

Ubiquitination plays a pivotal role in the surveillance of intracellular milieu and elimination of a variety of pathogens. Here, we identify bacterial ubiquitination substrates, surface-exposed proteins present on phylogenetically diverse bacteria including Streptococcus pneumoniae (SPN) and Salmonella enterica serovar Typhimurium (STm). By biochemical and intracellular survival study, we reveal a strategy utilized by the host to recognize such targets based on sensing of degron-like motifs. Such consensus degron-like motifs resemble intra-cytosolic PAMPs (Pathogen associated molecular patterns) to aid in the recognition of diverse microbes. Remarkably, by applying this approach, we could remodel and stimulate non-ubiquitin targets to be identified by host ligases. We also showcase a novel anti-microbial role of SCFFBXW7 complex supported by a regulatory host kinase, GSK3β in pathogenic elimination. In conclusion, our study suggests that the host exploits a universal set of rules for recognizing various pathogens to conserve cellular resources and boost anti-microbial immunity.

关键词

Bacterial pathogenesis, Cellular Immunity, Host-Pathogen interaction, Ubiquitination

研究领域

Biological Sciences, Microbiology

参考文献

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基金

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补充材料

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附加信息

利益冲突

No competing interests were disclosed.

数据可用性声明

The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.

知识共享许可协议

Copyright © 2023 Apte et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.