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Multi-regional comparative N-glycomics reveals both spatial and phylogenetic gradients in mammalian brain N-glycome complexity
发表日期 April 11, 2023 (DOI: https://doi.org/10.54985/peeref.2304p9434445)
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作者
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Thomas Klarić1 , Ivan Gudelj1 , Gabriel Santpere2 , André Sousa3 , Mislav Novokmet4 , Frano Vučković4 , Shaojie Ma1 , Ivona Bečeheli4 , Chet Sherwood5 , John Ely6 , Patrick Hof7 , Djuro Josić8 , Gordan Lauc4 , Nenad Sestan1
- Yale School of Medicine
- Universitat Pompeu Fabra
- University of Wisconsin Madison
- Genos, Zagreb, Croatia
- The George Washington University
- MAEBIOS, Alamogordo, USA
- Icahn School of Medicine at Mount Sinai
- Brown University
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会议/活动
- Federation of European Neuroscience Societies (FENS) regional meeting 2023, May 2023 (Algarve, Portugal)
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海报摘要
- Comparative “omics” studies have revealed unique aspects of human neurobiology, yet an evolutionary perspective of the brain N-glycome is lacking. Here, we performed multi-regional characterization of rat, macaque, chimpanzee, and human brain N-glycomes using chromatography and mass spectrometry, then integrated these data with complementary glycotranscriptomic data. We found that in primates the brain N-glycome has evolved more rapidly than the underlying transcriptomic framework, providing a means for generating additional inter-species diversity. We show that brain N-glycome evolution in hominids has been characterized by a global increase in complexity and a shift towards increased usage of α(2-6)-linked N-acetylneuraminic acid. As a result of overlapping anatomical and phylogenetic gradients in complex N-glycan abundance, peak N-glycome complexity was found in the hominid cortical regions. We hypothesize that this increased diversity and complexity of sugar modifications on neural N-glycoproteins contributed to the emergence of novel cognitive functions, including those unique to the human neocortex.
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关键词
- Brain, N-glycome, Evolution, N-glycomics, N-glycosylation, N-glycans
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研究领域
- Anatomy and Physiology, Biological Sciences, Neuroscience, Molecular and Cellular Biosciences, Evolutionary Biology
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参考文献
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基金
- 暂无数据
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补充材料
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- bioRxiv Manuscript Download
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附加信息
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- 利益冲突
- The authors declare the following competing interests: GL is the founder and owner of Genos Ltd, a private research organization that specializes in high-throughput glycomic analysis and has several patents in this field. TK, IG, MN, FV, and IB are currently or were employees of Genos Ltd while participating in this research project.
- 数据可用性声明
- The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.
- 知识共享许可协议
- Copyright © 2023 Klarić et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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引用
Klarić, T., Gudelj, I., Santpere, G., Sousa, A., Novokmet, M., Vučković, F., Ma, S., Bečeheli, I., Sherwood, C., Ely, J., Hof, P., Josić, D., Lauc, G., Sestan, N. Multi-regional comparative N-glycomics reveals both spatial and phylogenetic gradients in mammalian brain N-glycome complexity [not peer reviewed]. Peeref 2023 (poster).
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