期刊
STEM CELLS
卷 34, 期 3, 页码 640-652出版社
WILEY
DOI: 10.1002/stem.2278
关键词
Human salivary gland stem cells; Radiation induced xerostomia; Adult stem cells; Differentiation; Organoids
资金
- Netherlands Organisation for Health Research and Development (ZonMW) [11.600.1023]
- Netherlands Institute for Regenerative Medicine (NIRM) [FES0908]
- Dutch Cancer Society [RUG2008-4022]
Adult stem cells are often touted as therapeutic agents in the regenerative medicine field, however data detailing both the engraftment and functional capabilities of solid tissue derived human adult epithelial stem cells is scarce. Here we show the isolation of adult human salivary gland (SG) stem/progenitor cells and demonstrate at the single cell level in vitro self-renewal and differentiation into multilineage organoids. We also show in vivo functionality, long-term engraftment, and functional restoration in a xenotransplantation model. Indeed, transplanted human salisphere-derived cells restored saliva production and greatly improved the regenerative potential of irradiated SGs. Further selection for c-Kit expression enriched for cells with enhanced regenerative potencies. Interestingly, interaction of transplanted cells with the recipient SG may also be involved in functional recovery. Thus, we show for the first time that salispheres cultured from human SGs contain stem/progenitor cells capable of self-renewal and differentiation and rescue of saliva production. Our study underpins the therapeutic promise of salisphere cell therapy for the treatment of xerostomia.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据