期刊
MICROCHIMICA ACTA
卷 186, 期 6, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3465-y
关键词
Cyclic voltammetry; Differential pulse voltammetry; Potentiostatic-insertion method; 17-Estradiol detection; Ratiometric electrochemical aptasensor
资金
- National Natural Science Foundation of China [21471052, 21675049]
Gold nanoparticles (AuNPs) with a size of similar to 3nm were placed on a thionine-multiwalled carbon nanotube (Thi-CNTs) conjugate to form a novel AuNP-Thi-CNTs nanocomposite. Its morphology and composition were characterized by transmission electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The nanocomposite was placed on an electrode and used as a redox-active signaling interface to fabricate a ratiometric electrochemical aptasensor for 17-estradiol (E2). The potentiostatic insertion method was applied to insert an aptamer against E2 into a thin alkane monolayer to warrant an adequate distance between aptamers. The aptamer against E2 acts as both a collector and separator to specifically bind E2. The electrode displays two peak signals (at +0.50V vs. SCE for E2; and at -0.32V for Thi) which increase and decrease, respectively, in the 12 pM to 60nM E2 concentration range. Therefore, the current ratio can be used to reliably, reproducibly, and sensitively quantify the concentration of E2.
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