期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 124, 期 -, 页码 530-536出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.11.234
关键词
Laccase; Chitosan; Nanoencapsulation
资金
- Natural Sciences and Engineering Research Council of Canada [355254]
- Ministere des Relations Internationales du Quebec, Canada [122523]
- INRS-ETE
Laccase is one of the widely used enzymes for biotechnological processes. Immobilization of enzymes is a universally accepted approach to increase their reusability and stability. In this study, laccase enzyme from Trametes versicolor was encapsulated for the first time in a chitosan-nanobiochar matrix. The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration. The reusability of the encapsulated laccase was demonstrated towards the oxidation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) for several consecutive cycles, exhibiting 30% of the initial activity after 5 cycles. The encapsulated laccase showed a moderate increase in enzyme stability against pH and temperature variation compared to the free enzyme. Moreover, the storage stability of laccase at both 4 degrees C and 25 degrees C was increased after immobilization. Only 2% of laccase was leaked during a 5-day period from biocatalyst Laccase in its free form showed no antibacterial activity against Gram positive and Gram-negative model microorganisms, while encapsulated laccase showed antibacterial activity towards Gram-positive ones. Thus, the encapsulation of the laccase is an efficient method to keep the enzyme active and stable for different applications. (C) 2018 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据