期刊
THIN SOLID FILMS
卷 517, 期 15, 页码 4299-4302出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.tsf.2008.11.141
关键词
Laser deposition; Enzyme; Urease
类别
资金
- Hungarian-Romanian Governmental Bilateral Agreement Biosensors design using laser radiation
- Hungarian Scientific Research Foundation OTKA [K 67818]
- National Office for Research and Technology-Hungary NKTH [OMFB 00345/2006]
- Romanian Ministry for Education and Research [CEEX 150/2006]
Urease thin films were produced by Matrix Assisted Pulsed Laser Evaporation (MAPLE) and Pulsed Laser Deposition from two types of targets: frozen water solutions of urease with different concentrations (1-10% m/v) and pure urease pellets. The fluence of the ablating KrF excimer laser was varied between 300 and 2200 mJ/cm(2). Fourier transform infrared spectra of the deposited films showed no difference as compared to the original urease. Morphologic studies proved that the films consist of a smooth base layer with embedded micrometer-sized droplets. Absorption-coefficient measurements contradicted the traditional absorptive matrix model for MAPLE deposition. The laser energy was absorbed by urease clusters leading to a local heating-up and evaporation of the frozen matrix from the uppermost layer accompanied by the release of dissolved urease molecules. Significant enzymatic activity of urease was preserved only during matrix assisted transfer. (C) 2008 Elsevier B.V. All rights reserved.
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