期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 141, 期 1, 页码 293-300出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2009.06.006
关键词
Dielectrophoresis; Single-bead-based assay; Consecutive assay; Biochemical assay
资金
- National Science Council of Taiwan, ROC [96-2120-M-194004]
In this paper, we describe a method for performing single-bead-based, consecutive biochemical assays on a dielectrophoretic microfluidic chip using the interaction of FITC-labeled streptavidin with biotin-coated 2-mu m polystyrene (PS) beads as a model system. A microfluidic device with four sets of electrodes on a glass slide was used to trap a single bead at the center of each set of electrodes through negative dielectrophoresis. Each set of electrodes was composed of four electrodes arranged in a cross-like manner. The electrodes were prepared using a simple process consisting of UV lithography and aluminum etching. A laser-induced fluorescence system detected the fluorescence emission from the FITC-labeled streptavidin conjugated with the biotin-coated PS beads. We investigated the effects of(i) the magnification/numerical aperture of the objective used for collecting fluorescence signals and (ii) the cut-off frequency of the low-pass digital filter, which filtered out high-frequency noise, on the signal-to-noise ratio. The average measured fluorescence Maximum, determined from 10 consecutive fluorescence signals, increased with increasing concentrations of streptavidin (up to 0.3 mu g/mL): it leveled off when the concentration of streptavidin was greater than 0.3 mu g/mL. The limit of detection (signal-to-noise ratio = 3.0) was 8.3 ng/mL. (C) 2009 Elsevier B.V. All rights reserved.
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