期刊
PROTEIN SCIENCE
卷 20, 期 1, 页码 150-159出版社
WILEY-BLACKWELL
DOI: 10.1002/pro.547
关键词
intrinsically disordered proteins; site-directed spin labeling; SDSL; EPR; TFE; IA(3)
资金
- NSF [MCB-0746533 CAREER]
- Direct For Biological Sciences [0746533] Funding Source: National Science Foundation
Electron paramagnetic resonance (EPR) spectroscopy coupled with site-directed spin labeling (SDSL) is a valuable tool for characterizing the mobility and conformational changes of proteins but has seldom been applied to intrinsically disordered proteins (IDPs). Here, IA(3) is used as a model system demonstrating SDSL-EPR characterization of conformational changes in small IDP systems. IA(3) has 68 amino acids, is unstructured in solution, and becomes alpha-helical upon addition of the secondary structural stabilizer 2,2,2-trifluoroethanol (TFE). Two single cysteine substitutions, one in the N-terminus (S14C) and one in the C-terminus (N58C), were generated and labeled with three different nitroxide spin labels. The resultant EPR line shapes of each of the labels were compared and each reported changes in mobility upon addition of TFE. Specifically, the spectral line shape parameters h((+1))/h((0)), the local tumbling volume (V-L), and the percent change of the h((-1)) intensity were utilized to quantitatively monitor TFE-induced conformational changes. The values of h((+1))/h((0)) as a function of TFE titration varied in a sigmoidal manner and were fit to a two-state Boltzmann model that provided values for the midpoint of the transition, thus, reporting on the global conformational change of IA(3). The other parameters provide site-specific information and show that S14C-SL undergoes a conformational change resulting in more restricted motion than N58C-SL, which is consistent with previously published results obtained by studies using NMR and circular dichroism spectroscopy indicating a higher degree of alpha-helical propensity of the N-terminal segment of IA(3). Overall, the results provide a framework for data analyzes that can be used to study induced unstructured-to-helical conformations in IDPs by SDSL.
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