期刊
NANO LETTERS
卷 10, 期 3, 页码 788-792出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl9039718
关键词
Single molecule sequencing; phosphate-labeled nucleotide; DNA nanoball; SYBR; fluorescent
类别
资金
- NIH
- National Human Genome Research Institute [1-P01 HG003015]
Highly labeled DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates (dNTPs) were developed as a source of dNTPs for DNA polymerase The particles were prepared by strand-displacement polymerization from a self-complementary circular template. Imaged by atomic force microscopy, these functionalized particles appear as condensed fuzzy balls with diameters between 50 and 150 nm. They emit a bright fluorescent signal, detected in 2 MS exposures with a signal-to-noise ratio of 25 when imaged using a TIR fluorescence microscope.
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