Reliability Analysis of THz Characterization of Modified and Unmodified Vector Sequences

As part of a broad study to survey and parse the fundamental spectroscopic features of double stranded DNA in the THz domain, we have performed a comparative study of a 2900 bp basic sequence, 3200 bp basic sequence with inserts, and segments of the basic sequence of 1000 and 500 bp over the range 10-25 cm(-1). Samples were generated by amplifying regions of the firefly luciferase gene modified and unmodified pGEM vector sequences using the polymerase chain reaction. Rich spectra have been obtained from each type of sample at varying concentrations. Major features appear to be relatively unaffected by concentration, over the range of concentrations studied. The effect of inserts, filtering, and length of DNA was shown to be distinguishable. One of the spectra of each length was deconvoluted into about 25 separated bands.