Optimizing Three-Dimensional Bioprinting for Cell Culture Scaffolds

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作者

Emma Brudos¹ , Miranda Nelson² , David Estrada²

1. New Mexico Institute of Mining and Technology
2. Boise State University

会议/活动

Idaho Conference of Undergraduate Research, July 2022 (虚拟会议)

海报摘要

In order to increase the success rates of leukemia treatments, a greater understanding of cell interactions within the bone marrow is required. 3D bioprinting offers a solution for studying the cells in vitro by printing scaffolds that mimic the internal bone structure. Our research focused on evaluating a Cellink BioX printer in order to print custom scaffolds for 3D cell culture. These can be utilized in an environment that mimics the physiological conditions of trabecular bone to study treatments. The print conditions were optimized to produce scaffolds that had porosity and structure within the range of real human trabecular bone.

关键词

Bioprinting, Trabecular bone, Tissue engineering, Bone marrow, 3D cell culture

研究领域

Material Sciences, Bioengineering

参考文献

1. Barrett AJ, Battiwalla M. Relapse after allogeneic stem cell transplantation. Expert Rev Hematol. 2010 Aug;3(4):429-41. doi: 10.1586/ehm.10.32. PMID: 21083034; PMCID: PMC3426446.
2. National Cancer Institute. Definition of bone marrow. Retrieved from https://www.cancer.gov/publications/dictionaries/cancer-terms/def/bone-marrow
3. A cross section of a human long bone SVG file by Pbroks13, distributed under a Creative Commons Attribution 3.0 Unported license. Retrieved from https://commons.wikimedia.org/w/index.php?curid=5188772
4. O'Connell, C., et al. (2021). Characterizing Bioinks for Extrusion Bioprinting: Printability and Rheology. Methods in molecular biology. 2140:111-133 https://www.researchgate.net/publication/340110752_Characterizing_Bioinks_for_Extrusion_Bioprinting_Printability_and_Rheology
5. Lee, S., Porter, M., Wasko, S. et al. Potential Bone Replacement Materials Prepared by Two Methods. MRS Online Proceedings Library 1418, 177–188 (2012). https://doi.org/10.1557/opl.2012.671

基金

1. National Science Foundation (No. XC-1950305 and NSF-DMR 1710640)
2. National Science Foundation Career Award (No. 1848516)

补充材料

暂无数据

附加信息

利益冲突

None declared.

数据可用性声明

Data sharing not applicable to this poster as no datasets were generated or analyzed during the current study.

知识共享许可协议

Copyright © 2022 Brudos et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.