3D trapping and manipulation of micro-particles using holographic optical tweezers with optimized computer-generated holograms

A multi-plane adaptive-additive algorithm is developed for optimizing computer-generated holograms for the reconstruction of traps in three-dimensional (3D) spaces. This algorithm overcomes the converging stagnation problem of the traditional multi-plane Gerchberg-Saxton algorithm and improves the diffract; ion efficiency of the holograms effectively. The optimized holograms are applied in a holographic optical tweezers (HOT) platform. Additionally, a. computer program is developed and integrated into the HOT platform for the purpose of achieving the interactive control of traps. Experiments demonstrate that the manipulation of micro-particles into the 3D structure with optimized holograms can be carried out effectively on the HOT platform.