Journal
BIORESOURCE TECHNOLOGY
Volume 146, Issue -, Pages 807-811Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.08.032
Keywords
Cross-linked enzyme aggregates; Decolorization; Cloning laccase enzyme; Shewanella putrefaciens
Funding
- Fatih University Research Project Foundation [P50091005_2 (1400)]
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A novel type laccase from Shewanella putrefaciens was identified, expressed in Escherichia coli, characterized, prepared in cross-linked enzyme aggregates (CLEA) for industrial applications and investigated of decolorization activity on malchite green dye. Enzyme characterization was investigated by enzyme assay, SOS-PAGE and other biochemical reactions. Moreover, cross-linked enzyme aggregates were prepared and characterized. Saturated ammonium sulphate solution was used as the precipitating agent and cross linked with glutaraldehyde. These CLEA-laccase aggregates showed more catalytic efficiency and more stabilities compared to free laccase against harsh conditions of thermal and chemical agents as well as high reusability. Also it showed more decolorization ability. These results suggest that this CLEA is potentially usable in industrial applications. (C) 2013 Elsevier Ltd. All rights reserved.
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