Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 7, Pages 2549-2551Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02919-10
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Funding
- Cancer Research UK [C23949/A10945]
- BBSRC [BB/F003099/1, BB/D525056/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F003099/1, BB/D525056/1] Funding Source: researchfish
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We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors in Methanococcus maripaludis. Strain S0001, containing ORF1 from pURB500 integrated into the M. maripaludis genome, supports a significantly smaller shuttle vector, pAW42, and a 7,000-fold increase in transformation efficiency for pURB500-based vectors.
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